PHCA Antibody - #DF3761
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3761, RRID:AB_2836125.
折りたたみ／展開
ACER3; Alkaline CDase 3; Alkaline ceramidase 3; Alkaline dihydroceramidase SB89; Alkaline phytoceramidase; AlkCDase 3; aPHC; AV015045; FLJ11238; PHCA; phytoceramidase, alkaline;
免疫原
Ubiquitously expressed. Highly expressed in placenta (PubMed:11356846). Expressed in erythrocytes (PubMed:20207939).
- Q9NUN7 ACER3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAPAADREGYWGPTTSTLDWCEENYSVTWYIAEFWNTVSNLIMIIPPMFGAVQSVRDGLEKRYIASYLALTVVGMGSWCFHMTLKYEMQLLDELPMIYSCCIFVYCMFECFKIKNSVNYHLLFTLVLFSLIVTTVYLKVKEPIFHQVMYGMLVFTLVLRSIYIVTWVYPWLRGLGYTSLGIFLLGFLFWNIDNIFCESLRNFRKKVPPIIGITTQFHAWWHILTGLGSYLHILFSLYTRTLYLRYRPKVKFLFGIWPVILFEPLRKH
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NUN7 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T83 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| Y105 | Phosphorylation | Uniprot | |
| T124 | Phosphorylation | Uniprot | |
| T133 | Phosphorylation | Uniprot |
研究背景
Endoplasmic reticulum and Golgi ceramidase that catalyzes the hydrolysis of unsaturated long-chain C18:1-, C20:1- and C20:4-ceramides, dihydroceramides and phytoceramides into sphingoid bases like sphingosine and free fatty acids at alkaline pH. Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation. Controls the generation of sphingosine in erythrocytes, and thereby sphingosine-1-phosphate in plasma. Through the regulation of ceramides and sphingosine-1-phosphate homeostasis in the brain may play a role in neurons survival and function (By similarity). By regulating the levels of proinflammatory ceramides in immune cells and tissues, may modulate the inflammatory response (By similarity).
Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein.
Ubiquitously expressed. Highly expressed in placenta. Expressed in erythrocytes.
Belongs to the alkaline ceramidase family.
研究領域
· Metabolism > Lipid metabolism > Sphingolipid metabolism.
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