製品: ATP5I Antibody
カタログ: DF3803
タンパク質の説明: Rabbit polyclonal antibody to ATP5I
アプリケーション: WB IF/ICC
反応性: Human
分子量: 8 KD; 8kD(Calculated).
ユニプロット: P56385
RRID: AB_2836160

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Polyclonal
特異性:
ATP5I Antibody detects endogenous levels of total ATP5I.
RRID:
AB_2836160
引用形式: Affinity Biosciences Cat# DF3803, RRID:AB_2836160.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

ATP 5I; ATP 5K; ATP synthase e chain mitochondrial; ATP synthase H+ transporting mitochondrial F0 complex subunit E; ATP synthase subunit e; ATP synthase subunit e mitochondrial; ATP5I; ATP5I_HUMAN; ATP5K; ATPase subunit e; F1F0 ATP synthase murine e subunit; MGC12532; mitochondrial;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MVPPVQVSPLIKLGRYSALFLGVAYGATRYNYLKPRAEEERRIAAEEKKKQDELKRIARELAEDDSILK

PTMs - P56385 基板として

Site PTM Type Enzyme
S8 Phosphorylation
Y25 Phosphorylation
T28 Phosphorylation
Y30 Phosphorylation
Y32 Phosphorylation
K34 Acetylation
K34 Ubiquitination
K49 Ubiquitination
K50 Ubiquitination
S66 Phosphorylation
K69 Acetylation
K69 Ubiquitination

研究背景

機能:

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.

細胞の位置付け:

Mitochondrion. Mitochondrion inner membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(0) seems to have nine subunits: a, b, c, d, e, f, g, F6 and 8 (or A6L). Component of an ATP synthase complex composed of ATP5PB, ATP5MC1, ATP5F1E, ATP5PD, ATP5ME, ATP5PF, ATP5MF, MT-ATP6, MT-ATP8, ATP5F1A, ATP5F1B, ATP5F1D, ATP5F1C, ATP5PO, ATP5MG, ATP5MD and ATP5MPL (By similarity).

タンパク質ファミリー:

Belongs to the ATPase e subunit family.

研究領域

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

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