DDX19B Antibody - #DF3817
| 製品: | DDX19B Antibody |
| カタログ: | DF3817 |
| タンパク質の説明: | Rabbit polyclonal antibody to DDX19B |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Chicken |
| 分子量: | 50 KD; 54kD(Calculated). |
| ユニプロット: | Q9UMR2 |
| RRID: | AB_2836174 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3817, RRID:AB_2836174.
折りたたみ／展開
ATP-dependent RNA helicase DDX19B; DBP5; Dbp5, yeast, homolog of; DD19B_HUMAN; DDX19; DDX19B; DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B; DEAD box protein 19B; DEAD box RNA helicase DEAD5; DEAD-box protein 5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5, yeast, homolog); DEAD/H BOX 19; helicase DEAD5; RNAh; TDBP; yeast Dbp5 homolog;
免疫原
- Q9UMR2 DD19B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATDSWALAVDEQEAAAESLSNLHLKEEKIKPDTNGAVVKTNANAEKTDEEEKEDRAAQSLLNKLIRSNLVDNTNQVEVLQRDPNSPLYSVKSFEELRLKPQLLQGVYAMGFNRPSKIQENALPLMLAEPPQNLIAQSQSGTGKTAAFVLAMLSQVEPANKYPQCLCLSPTYELALQTGKVIEQMGKFYPELKLAYAVRGNKLERGQKISEQIVIGTPGTVLDWCSKLKFIDPKKIKVFVLDEADVMIATQGHQDQSIRIQRMLPRNCQMLLFSATFEDSVWKFAQKVVPDPNVIKLKREEETLDTIKQYYVLCSSRDEKFQALCNLYGAITIAQAMIFCHTRKTASWLAAELSKEGHQVALLSGEMMVEQRAAVIERFREGKEKVLVTTNVCARGIDVEQVSVVINFDLPVDKDGNPDNETYLHRIGRTGRFGKRGLAVNMVDSKHSMNILNRIQEHFNKKIERLDTDDLDEIEKIAN
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UMR2 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| T48 | Phosphorylation | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| Y89 | Phosphorylation | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| S93 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S154 | Phosphorylation | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| R199 | Methylation | Uniprot | |
| K202 | Acetylation | Uniprot | |
| K229 | Acetylation | Uniprot | |
| K287 | Acetylation | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| K308 | Ubiquitination | Uniprot | |
| T422 | Phosphorylation | Uniprot | |
| Y423 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| T468 | Phosphorylation | Uniprot | |
| K476 | Ubiquitination | Uniprot |
研究背景
ATP-dependent RNA helicase involved in mRNA export from the nucleus. Rather than unwinding RNA duplexes, DDX19B functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins.
Cytoplasm. Nucleus>Nuclear pore complex. Nucleus membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Nuclear pore complex cytoplasmic fibrils.
Interacts with NUP214 or RNA in a mutually exclusive manner.
The N-terminal extension helix acts as an autoinhibitory domain, preventing ATP hydrolysis, unless the N-terminus of the protein is displaced by RNA binding, allowing cleft closure to bring key side chains into position for catalysis.
Belongs to the DEAD box helicase family. DDX19/DBP5 subfamily.
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