製品: B3GALT1 Antibody
カタログ: DF3833
タンパク質の説明: Rabbit polyclonal antibody to B3GALT1
アプリケーション: WB IF/ICC
反応性: Human, Mouse
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 36 KD; 38kD(Calculated).
ユニプロット: Q9Y5Z6
RRID: AB_2836190

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%)
クローナリティ:
Polyclonal
特異性:
B3GALT1 Antibody detects endogenous levels of total B3GALT1.
RRID:
AB_2836190
引用形式: Affinity Biosciences Cat# DF3833, RRID:AB_2836190.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

3-galactosyltransferase 1; 3-GalTase 1; B3GALT1; B3GT1_HUMAN; Beta 1,3 galactosyltransferase 1; Beta 1,3 GalTase 1; Beta 3 galt1; Beta-1; beta3Gal T1; Beta3Gal-T1; Beta3GalT1; UDP Gal:betaGlcNAc beta 1,3 galactosyltransferase, polypeptide 1; UDP galactose:beta N acetyl glucosamine beta 1,3 galactosyltransferase 1; UDP-galactose:beta-N-acetyl-glucosamine-beta-1;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q9Y5Z6 B3GT1_HUMAN:

Detected in brain and colon mucosa and to a lesser extent in colon adenocarcinoma cells.

タンパク質配列:
MASKVSCLYVLTVVCWASALWYLSITRPTSSYTGSKPFSHLTVARKNFTFGNIRTRPINPHSFEFLINEPNKCEKNIPFLVILISTTHKEFDARQAIRETWGDENNFKGIKIATLFLLGKNADPVLNQMVEQESQIFHDIIVEDFIDSYHNLTLKTLMGMRWVATFCSKAKYVMKTDSDIFVNMDNLIYKLLKPSTKPRRRYFTGYVINGGPIRDVRSKWYMPRDLYPDSNYPPFCSGTGYIFSADVAELIYKTSLHTRLLHLEDVYVGLCLRKLGIHPFQNSGFNHWKMAYSLCRYRRVITVHQISPEEMHRIWNDMSSKKHLRC

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
92
Chicken
80
Xenopus
70
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y5Z6 基板として

Site PTM Type Enzyme
T114 Phosphorylation
K190 Methylation

研究背景

機能:

Beta-1,3-galactosyltransferase that transfers galactose from UDP-alpha-D-galactose to substrates with a terminal beta-N-acetylglucosamine (beta-GlcNAc) residue. Involved in the biosynthesis of the carbohydrate moieties of glycolipids and glycoproteins. Inactive towards substrates with terminal alpha-N-acetylglucosamine (alpha-GlcNAc) or alpha-N-acetylgalactosamine (alpha-GalNAc) residues.

細胞の位置付け:

Golgi apparatus membrane>Single-pass type II membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in brain and colon mucosa and to a lesser extent in colon adenocarcinoma cells.

タンパク質ファミリー:

Belongs to the glycosyltransferase 31 family.

研究領域

· Metabolism > Glycan biosynthesis and metabolism > Glycosphingolipid biosynthesis - lacto and neolacto series.

· Metabolism > Global and overview maps > Metabolic pathways.

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