製品: B4GALT5 Antibody
カタログ: DF3841
タンパク質の説明: Rabbit polyclonal antibody to B4GALT5
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 40 KD; 45kD(Calculated).
ユニプロット: O43286
RRID: AB_2836198

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(82%), Bovine(82%), Horse(82%), Sheep(82%), Rabbit(100%), Dog(100%), Chicken(91%)
クローナリティ:
Polyclonal
特異性:
B4GALT5 Antibody detects endogenous levels of total B4GALT5.
RRID:
AB_2836198
引用形式: Affinity Biosciences Cat# DF3841, RRID:AB_2836198.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

4-galactosyltransferase 5; 4-GalT II; 4-GalTase 5; B4Gal T5; b4Gal-T5; B4galt5; B4GT5_HUMAN; Beta 1 4 galactosyltransferase 5; Beta 1 4 galactosyltransferase V; Beta 1 4 GalT II; Beta 1 4 GalT IV; Beta 1 4 GalTase 5; Beta-1; Beta-1,4-galactosyltransferase 5; Beta-1,4-GalT II; Beta-1,4-GalTase 5; Beta4 GALT IV; Beta4Gal T5; Beta4Gal-T5; Beta4GalT V; gt V; gtV; MGC138470; UDP Gal:beta GlcNAc beta 1 4 galactosyltransferase 5; UDP Gal:betaGlcNAc beta 1 4 galactosyltransferase 5; UDP Gal:betaGlcNAc beta 1 4 galactosyltransferase polypeptide 5; UDP galactose:beta N acetylglucosamine beta 1 4 galactosyltransferase 5; UDP-Gal:beta-GlcNAc beta-1; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5; UDP-galactose:beta-N-acetylglucosamine beta-1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
O43286 B4GT5_HUMAN:

Ubiquitously expressed.

タンパク質配列:
MRARRGLLRLPRRSLLAALFFFSLSSSLLYFVYVAPGIVNTYLFMMQAQGILIRDNVRTIGAQVYEQVLRSAYAKRNSSVNDSDYPLDLNHSETFLQTTTFLPEDFTYFANHTCPERLPSMKGPIDINMSEIGMDYIHELFSKDPTIKLGGHWKPSDCMPRWKVAILIPFRNRHEHLPVLFRHLLPMLQRQRLQFAFYVVEQVGTQPFNRAMLFNVGFQEAMKDLDWDCLIFHDVDHIPESDRNYYGCGQMPRHFATKLDKYMYLLPYTEFFGGVSGLTVEQFRKINGFPNAFWGWGGEDDDLWNRVQNAGYSVSRPEGDTGKYKSIPHHHRGEVQFLGRYALLRKSKERQGLDGLNNLNYFANITYDALYKNITVNLTPELAQVNEY

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Rabbit
100
Chicken
91
Horse
82
Bovine
82
Sheep
82
Zebrafish
82
Xenopus
73
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43286 基板として

Site PTM Type Enzyme
T59 O-Glycosylation
T59 Phosphorylation
S71 Phosphorylation
Y73 Phosphorylation
K323 Ubiquitination
T375 Phosphorylation
Y388 Phosphorylation

研究背景

機能:

Catalyzes the synthesis of lactosylceramide (LacCer) via the transfer of galactose from UDP-galactose to glucosylceramide (GlcCer). LacCer is the starting point in the biosynthesis of all gangliosides (membrane-bound glycosphingolipids) which play pivotal roles in the CNS including neuronal maturation and axonal and myelin formation (By similarity). Plays a role in the glycosylation of BMPR1A and regulation of its protein stability (By similarity). Essential for extraembryonic development during early embryogenesis (By similarity).

細胞の位置付け:

Golgi apparatus>Golgi stack membrane>Single-pass type II membrane protein. Golgi apparatus.
Note: Trans cisternae of Golgi stack.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitously expressed.

タンパク質ファミリー:

Belongs to the glycosyltransferase 7 family.

研究領域

· Metabolism > Glycan biosynthesis and metabolism > Mucin type O-glycan biosynthesis.

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