ARFGEF2 Antibody - #DF3857
| 製品: | ARFGEF2 Antibody |
| カタログ: | DF3857 |
| タンパク質の説明: | Rabbit polyclonal antibody to ARFGEF2 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Horse, Rabbit, Dog |
| 分子量: | 210 KD; 202kD(Calculated). |
| ユニプロット: | Q9Y6D5 |
| RRID: | AB_2836214 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3857, RRID:AB_2836214.
折りたたみ／展開
ADP ribosylation factor guanine nucleotide exchange factor 2 (brefeldin A inhibited); ADP ribosylation factor guanine nucleotide exchange factor 2; ARFGEF 2; ARFGEF2; ARFGEP2; BIG 2; BIG2; Brefeldin A inhibited 2; Brefeldin A inhibited GEP 2; Brefeldin A inhibited guanine nucleotide exchange protein 2; dJ1164I10.1; FLJ23723;
免疫原
- Q9Y6D5 BIG2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAEIEKQRLGTAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETICSCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEKPIQSKPQSPVIQAAAVSPKFVRLKHSQAQSKPTTPEKTDLTNGEHARSDSGKVSTENGDAPRERGSSLSGTDDGAQEVVKDILEDVVTSAIKEAAEKHGLTEPERVLGELECQECAIPPGVDENSQTNGIADDRQSLSSADNLESDAQGHQVAARFSHVLQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRTHEMFINAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQTSLGQERLTDQEIGDGKGLDMARRCSVTSMESTVSSGTQTTVQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGTSVEDIAQFLHQEERLDSTQVGDFLGDSARFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYEEIEGKKIAMKETKELTIATKSTKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAIRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHTLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMAIRCIAQMVNSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTCHIVTTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFEKHWWQDLFRIVFRIFDNMKLPEQLSEKSEWMTTTCNHALYAICDVFTQFYEALNEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPEVWDETCNCMLDIFKTTIPHVLLTWRPVGMEEDSSEKHLDVDLDRQSLSSIDKNPSERGQSQLSNPTDDSWKGRPYANQKLFASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDADIHIETEDQGMYKYMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDENRRDSWEEIQQRLLTVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKINDEKFKAHASMYYPYLCEIMQFDLIPELRAVLRKFFLRIGVVYKIWIPEEPSQVPAALSPVW
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y6D5 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K17 | Acetylation | Uniprot | |
| K22 | Acetylation | Uniprot | |
| K22 | Ubiquitination | Uniprot | |
| K25 | Acetylation | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| T101 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| S218 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| T243 | Phosphorylation | Uniprot | |
| T244 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| S279 | Phosphorylation | Uniprot | |
| T281 | Phosphorylation | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| S346 | Phosphorylation | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| K383 | Acetylation | Uniprot | |
| T424 | Phosphorylation | Uniprot | |
| S569 | Phosphorylation | Uniprot | |
| T589 | Phosphorylation | Uniprot | |
| S590 | Phosphorylation | Uniprot | |
| S614 | Phosphorylation | Uniprot | |
| T616 | Phosphorylation | Uniprot | |
| S617 | Phosphorylation | Uniprot | |
| S620 | Phosphorylation | Uniprot | |
| T621 | Phosphorylation | Uniprot | |
| S690 | Phosphorylation | Uniprot | |
| T691 | Phosphorylation | Uniprot | |
| S700 | Phosphorylation | Uniprot | |
| K741 | Ubiquitination | Uniprot | |
| K794 | Acetylation | Uniprot | |
| Y797 | Phosphorylation | Uniprot | |
| K808 | Ubiquitination | Uniprot | |
| K840 | Ubiquitination | Uniprot | |
| T866 | Phosphorylation | Uniprot | |
| S875 | Phosphorylation | Uniprot | |
| T882 | Phosphorylation | Uniprot | |
| S883 | Phosphorylation | Uniprot | |
| T885 | Phosphorylation | Uniprot | |
| T1007 | Phosphorylation | Uniprot | |
| K1010 | Ubiquitination | Uniprot | |
| S1015 | Phosphorylation | Uniprot | |
| S1024 | Phosphorylation | Uniprot | |
| T1029 | Phosphorylation | Uniprot | |
| S1064 | Phosphorylation | Uniprot | |
| S1067 | Phosphorylation | Uniprot | |
| S1079 | Phosphorylation | Uniprot | |
| K1167 | Ubiquitination | Uniprot | |
| K1171 | Ubiquitination | Uniprot | |
| K1181 | Ubiquitination | Uniprot | |
| S1498 | Phosphorylation | Uniprot | |
| S1499 | Phosphorylation | Uniprot | |
| S1511 | Phosphorylation | Uniprot | |
| S1513 | Phosphorylation | Uniprot | |
| S1514 | Phosphorylation | Uniprot | |
| K1517 | Ubiquitination | Uniprot | |
| S1520 | Phosphorylation | Uniprot | |
| S1525 | Phosphorylation | Uniprot | |
| S1528 | Phosphorylation | Uniprot | |
| T1531 | Phosphorylation | Uniprot | |
| S1534 | Phosphorylation | Uniprot | |
| K1536 | Ubiquitination | Uniprot | |
| Y1632 | Phosphorylation | Uniprot | |
| K1727 | Ubiquitination | Uniprot | |
| S1782 | Phosphorylation | Uniprot |
研究背景
Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extent on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles; the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways.
In vitro phosphorylated by PKA reducing its GEF activity and dephosphorylated by phosphatase PP1.
Cytoplasm. Membrane. Golgi apparatus. Cytoplasm>Perinuclear region. Golgi apparatus>trans-Golgi network. Endosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cell projection>Dendrite. Cytoplasmic vesicle. Cell junction>Synapse. Cytoplasm>Cytoskeleton.
Note: Translocates from cytoplasm to membranes upon cAMP treatment. Localized in recycling endosomes.
Expressed in placenta, lung, heart, brain, kidney and pancreas.
Homodimer (Probable). Interacts with ARFGEF1/BIG1; both proteins are probably part of the same or very similar macromolecular complexes. Interacts with PRKAR1A, PRKAR2A, PRKAR1B, PRKAR2B, PPP1CC, PDE3A, TNFRSF1A, MYCBP and EXOC7. Interacts with GABRB1, GABRB2 and GABRB3 (By similarity).
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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