BAZ2A Antibody - #DF3858
| 製品: | BAZ2A Antibody |
| カタログ: | DF3858 |
| タンパク質の説明: | Rabbit polyclonal antibody to BAZ2A |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 210 KD; 211kD(Calculated). |
| ユニプロット: | Q9UIF9 |
| RRID: | AB_2836215 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3858, RRID:AB_2836215.
免疫原
Expressed at moderate levels in most tissues analyzed, including heart, brain, placenta, lung, skeletal muscle, kidney and pancreas.
- Q9UIF9 BAZ2A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEMEANDHFNFTGLPPAPAASGLKPSPSSGEGLYTNGSPMNFPQQGKSLNGDVNVNGLSTVSHTTTSGILNSAPHSSSTSHLHHPSVAYDCLWNYSQYPSANPGSNLKDPPLLSQFSGGQYPLNGILGGSRQPSSPSHNTNLRAGSQEFWANGTQSPMGLNFDSQELYDSFPDQNFEVMPNGPPSFFTSPQTSPMLGSSIQTFAPSQEVGSGIHPDEAAEKEMTSVVAENGTGLVGSLELEEEQPELKMCGYNGSVPSVESLHQEVSVLVPDPTVSCLDDPSHLPDQLEDTPILSEDSLEPFNSLAPEPVSGGLYGIDDTELMGAEDKLPLEDSPVISALDCPSLNNATAFSLLADDSQTSTSIFASPTSPPVLGESVLQDNSFDLNNGSDAEQEEMETQSSDFPPSLTQPAPDQSSTIQLHPATSPAVSPTTSPAVSLVVSPAASPEISPEVCPAASTVVSPAVFSVVSPASSAVLPAVSLEVPLTASVTSPKASPVTSPAAAFPTASPANKDVSSFLETTADVEEITGEGLTASGSGDVMRRRIATPEEVRLPLQHGWRREVRIKKGSHRWQGETWYYGPCGKRMKQFPEVIKYLSRNVVHSVRREHFSFSPRMPVGDFFEERDTPEGLQWVQLSAEEIPSRIQAITGKRGRPRNTEKAKTKEVPKVKRGRGRPPKVKITELLNKTDNRPLKKLEAQETLNEEDKAKIAKSKKKMRQKVQRGECQTTIQGQARNKRKQETKSLKQKEAKKKSKAEKEKGKTKQEKLKEKVKREKKEKVKMKEKEEVTKAKPACKADKTLATQRRLEERQRQQMILEEMKKPTEDMCLTDHQPLPDFSRVPGLTLPSGAFSDCLTIVEFLHSFGKVLGFDPAKDVPSLGVLQEGLLCQGDSLGEVQDLLVRLLKAALHDPGFPSYCQSLKILGEKVSEIPLTRDNVSEILRCFLMAYGVEPALCDRLRTQPFQAQPPQQKAAVLAFLVHELNGSTLIINEIDKTLESMSSYRKNKWIVEGRLRRLKTVLAKRTGRSEVEMEGPEECLGRRRSSRIMEETSGMEEEEEEESIAAVPGRRGRRDGEVDATASSIPELERQIEKLSKRQLFFRKKLLHSSQMLRAVSLGQDRYRRRYWVLPYLAGIFVEGTEGNLVPEEVIKKETDSLKVAAHASLNPALFSMKMELAGSNTTASSPARARGRPRKTKPGSMQPRHLKSPVRGQDSEQPQAQLQPEAQLHAPAQPQPQLQLQLQSHKGFLEQEGSPLSLGQSQHDLSQSAFLSWLSQTQSHSSLLSSSVLTPDSSPGKLDPAPSQPPEEPEPDEAESSPDPQALWFNISAQMPCNAAPTPPPAVSEDQPTPSPQQLASSKPMNRPSAANPCSPVQFSSTPLAGLAPKRRAGDPGEMPQSPTGLGQPKRRGRPPSKFFKQMEQRYLTQLTAQPVPPEMCSGWWWIRDPEMLDAMLKALHPRGIREKALHKHLNKHRDFLQEVCLRPSADPIFEPRQLPAFQEGIMSWSPKEKTYETDLAVLQWVEELEQRVIMSDLQIRGWTCPSPDSTREDLAYCEHLSDSQEDITWRGRGREGLAPQRKTTNPLDLAVMRLAALEQNVERRYLREPLWPTHEVVLEKALLSTPNGAPEGTTTEISYEITPRIRVWRQTLERCRSAAQVCLCLGQLERSIAWEKSVNKVTCLVCRKGDNDEFLLLCDGCDRGCHIYCHRPKMEAVPEGDWFCTVCLAQQVEGEFTQKPGFPKRGQKRKSGYSLNFSEGDGRRRRVLLRGRESPAAGPRYSEEGLSPSKRRRLSMRNHHSDLTFCEIILMEMESHDAAWPFLEPVNPRLVSGYRRIIKNPMDFSTMRERLLRGGYTSSEEFAADALLVFDNCQTFNEDDSEVGKAGHIMRRFFESRWEEFYQGKQANL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UIF9 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S38 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| S496 | Phosphorylation | Uniprot | |
| S500 | Phosphorylation | Uniprot | |
| T507 | Phosphorylation | Uniprot | |
| S509 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T548 | Phosphorylation | Uniprot | |
| K585 | Acetylation | Uniprot | |
| K585 | Methylation | Uniprot | |
| K595 | Ubiquitination | Uniprot | |
| S604 | Phosphorylation | Uniprot | |
| S613 | Phosphorylation | Uniprot | |
| T649 | Phosphorylation | Uniprot | |
| K680 | Acetylation | Uniprot | |
| K695 | Sumoylation | Uniprot | |
| T701 | Phosphorylation | Uniprot | |
| K737 | Acetylation | Uniprot | |
| K739 | Acetylation | Uniprot | |
| K767 | Acetylation | Uniprot | |
| S928 | Phosphorylation | Uniprot | |
| T995 | Phosphorylation | Uniprot | |
| K1006 | Acetylation | Uniprot | |
| T1024 | Phosphorylation | Uniprot | |
| S1027 | Phosphorylation | Uniprot | |
| S1043 | Phosphorylation | Uniprot | |
| S1044 | Phosphorylation | Uniprot | |
| T1050 | Phosphorylation | Uniprot | |
| S1051 | Phosphorylation | Uniprot | |
| K1150 | Sumoylation | Uniprot | |
| K1172 | Sumoylation | Uniprot | |
| S1183 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S1184 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S1207 | Phosphorylation | Uniprot | |
| R1210 | Methylation | Uniprot | |
| S1343 | Phosphorylation | Uniprot | |
| S1370 | Phosphorylation | Uniprot | |
| S1376 | Phosphorylation | Uniprot | |
| T1377 | Phosphorylation | Uniprot | |
| S1397 | Phosphorylation | Uniprot | |
| T1399 | Phosphorylation | Uniprot | |
| K1467 | Ubiquitination | Uniprot | |
| S1542 | Phosphorylation | Uniprot | |
| S1559 | Phosphorylation | Uniprot | |
| T1621 | Phosphorylation | Uniprot | |
| K1672 | Ubiquitination | Uniprot | |
| K1676 | Ubiquitination | Uniprot | |
| K1746 | Acetylation | Uniprot | |
| S1747 | Phosphorylation | Uniprot | |
| S1750 | Phosphorylation | Uniprot | |
| S1754 | Phosphorylation | Uniprot | |
| R1768 | Methylation | Uniprot | |
| S1770 | Phosphorylation | Uniprot | |
| R1776 | Methylation | Uniprot | |
| Y1777 | Phosphorylation | Uniprot | |
| S1778 | Phosphorylation | Uniprot | |
| S1783 | Phosphorylation | Uniprot | |
| S1785 | Phosphorylation | Uniprot | |
| K1786 | Ubiquitination | Uniprot | |
| R1787 | Methylation | Uniprot | |
| K1901 | Ubiquitination | Uniprot |
研究背景
Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. In the complex, it plays a central role by being recruited to rDNA and by targeting chromatin modifying enzymes such as HDAC1, leading to repress RNA polymerase I transcription. Recruited to rDNA via its interaction with TTF1 and its ability to recognize and bind histone H4 acetylated on 'Lys-16' (H4K16ac), leading to deacetylation of H4K5ac, H4K8ac, H4K12ac but not H4K16ac. Specifically binds pRNAs, 150-250 nucleotide RNAs that are complementary in sequence to the rDNA promoter; pRNA-binding is required for heterochromatin formation and rDNA silencing (By similarity).
Acetylation at Lys-680 by KAT8/MOF promotes its dissociation from pRNA, affecting heterochromatin formation, nucleosome positioning and rDNA silencing. Deacetylation by SIRT1 in late S phase enhances pRNA-binding, allowing de novo DNA methylation and heterochromatin formation. Acetylation is high during S phase and declines to background levels in late S phase when the silent copies of rRNA genes are replicated (By similarity).
Ubiquitinated. Deubiquitinated by USP21 leading to its stabilization.
Nucleus>Nucleolus.
Note: Colocalizes with the basal RNA polymerase I transcription factor UBF in the nucleolus.
Expressed at moderate levels in most tissues analyzed, including heart, brain, placenta, lung, skeletal muscle, kidney and pancreas.
Component of the NoRC complex, at least composed of SMARCA5/SNF2H and BAZ2A/TIP5. Interacts with TTF1; required for recruitment of the NoRC complex to rDNA. Interacts with DNMT1, DNM3B, HDAC1 and SIN3A (By similarity). Interacts with BEND3 and USP21.
The bromo domain and the PHD-type zinc finger recognize and bind histone H4 acetylated on 'Lys-16' (H4K16ac). These 2 domains play a central role in the recruitment of chromatin silencing proteins such as DNMT1, DNMT3B and HDAC1.
The MBD (methyl-CpG-binding) domain, also named TAM domain, specifically recognizes and binds a conserved stem-loop structure the association within pRNA. Binding to pRNA induces a conformational change of BAZ2A/TIP5 and is essential for targeting the NoRC complex to the nucleolus (By similarity).
Belongs to the WAL family.
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