ABCC2 Antibody - #DF3873

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製品: ABCC2 Antibody
カタログ: DF3873
タンパク質の説明: Rabbit polyclonal antibody to ABCC2
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 174 KD; 174kD(Calculated).
ユニプロット: Q92887
RRID: AB_2836226

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(80%), Bovine(80%), Horse(90%), Sheep(80%), Rabbit(80%), Dog(90%), Xenopus(90%)
クローナリティ:
Polyclonal
特異性:
ABCC2 Antibody detects endogenous levels of total ABCC2.
RRID:
AB_2836226
引用形式: Affinity Biosciences Cat# DF3873, RRID:AB_2836226.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ABC30; abcC2; ATP binding cassette sub family C (CFTR/MRP) member 2; ATP binding cassette subfamily C member 2; ATP-binding cassette sub-family C member 2; Canalicular multidrug resistance protein; Canalicular multispecific organic anion transporter 1; CMOAT; CMOAT1; cMRP; DJS; KIAA1010; MRP 2; MRP2_HUMAN; Multidrug resistance associated protein 2; Multidrug resistance-associated protein 2;

免疫原

免疫原:
Uniprot:

Q92887(MRP2_HUMAN) >>Visit HPA database.

遺伝子(ID):
ABCC2(1244)
発現特異性:
Q92887 MRP2_HUMAN:

Expressed by polarized cells in liver, kidney and intestine. The highest expression is found in liver.

タンパク質配列:
MLEKFCNSTFWNSSFLDSPEADLPLCFEQTVLVWIPLGYLWLLAPWQLLHVYKSRTKRSSTTKLYLAKQVFVGFLLILAAIELALVLTEDSGQATVPAVRYTNPSLYLGTWLLVLLIQYSRQWCVQKNSWFLSLFWILSILCGTFQFQTLIRTLLQGDNSNLAYSCLFFISYGFQILILIFSAFSENNESSNNPSSIASFLSSITYSWYDSIILKGYKRPLTLEDVWEVDEEMKTKTLVSKFETHMKRELQKARRALQRRQEKSSQQNSGARLPGLNKNQSQSQDALVLEDVEKKKKKSGTKKDVPKSWLMKALFKTFYMVLLKSFLLKLVNDIFTFVSPQLLKLLISFASDRDTYLWIGYLCAILLFTAALIQSFCLQCYFQLCFKLGVKVRTAIMASVYKKALTLSNLARKEYTVGETVNLMSVDAQKLMDVTNFMHMLWSSVLQIVLSIFFLWRELGPSVLAGVGVMVLVIPINAILSTKSKTIQVKNMKNKDKRLKIMNEILSGIKILKYFAWEPSFRDQVQNLRKKELKNLLAFSQLQCVVIFVFQLTPVLVSVVTFSVYVLVDSNNILDAQKAFTSITLFNILRFPLSMLPMMISSMLQASVSTERLEKYLGGDDLDTSAIRHDCNFDKAMQFSEASFTWEHDSEATVRDVNLDIMAGQLVAVIGPVGSGKSSLISAMLGEMENVHGHITIKGTTAYVPQQSWIQNGTIKDNILFGTEFNEKRYQQVLEACALLPDLEMLPGGDLAEIGEKGINLSGGQKQRISLARATYQNLDIYLLDDPLSAVDAHVGKHIFNKVLGPNGLLKGKTRLLVTHSMHFLPQVDEIVVLGNGTIVEKGSYSALLAKKGEFAKNLKTFLRHTGPEEEATVHDGSEEEDDDYGLISSVEEIPEDAASITMRRENSFRRTLSRSSRSNGRHLKSLRNSLKTRNVNSLKEDEELVKGQKLIKKEFIETGKVKFSIYLEYLQAIGLFSIFFIILAFVMNSVAFIGSNLWLSAWTSDSKIFNSTDYPASQRDMRVGVYGALGLAQGIFVFIAHFWSAFGFVHASNILHKQLLNNILRAPMRFFDTTPTGRIVNRFAGDISTVDDTLPQSLRSWITCFLGIISTLVMICMATPVFTIIVIPLGIIYVSVQMFYVSTSRQLRRLDSVTRSPIYSHFSETVSGLPVIRAFEHQQRFLKHNEVRIDTNQKCVFSWITSNRWLAIRLELVGNLTVFFSALMMVIYRDTLSGDTVGFVLSNALNITQTLNWLVRMTSEIETNIVAVERITEYTKVENEAPWVTDKRPPPDWPSKGKIQFNNYQVRYRPELDLVLRGITCDIGSMEKIGVVGRTGAGKSSLTNCLFRILEAAGGQIIIDGVDIASIGLHDLREKLTIIPQDPILFSGSLRMNLDPFNNYSDEEIWKALELAHLKSFVASLQLGLSHEVTEAGGNLSIGQRQLLCLGRALLRKSKILVLDEATAAVDLETDNLIQTTIQNEFAHCTVITIAHRLHTIMDSDKVMVLDNGKIIECGSPEELLQIPGPFYFMAKEAGIENVNSTKF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
90
Dog
90
Xenopus
90
Pig
80
Bovine
80
Sheep
80
Rabbit
80
Chicken
60
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92887 基板として

Site PTM Type Enzyme
S60 Phosphorylation
K63 Ubiquitination
Y65 Phosphorylation
K236 Acetylation
K236 Ubiquitination
T237 Phosphorylation
K241 Ubiquitination
K247 Ubiquitination
K278 Ubiquitination
S281 Phosphorylation
S283 Phosphorylation
K294 Ubiquitination
K298 Acetylation
K302 Acetylation
K307 Acetylation
K403 Ubiquitination
K413 Ubiquitination
K485 Ubiquitination
K490 Ubiquitination
K500 Ubiquitination
K510 Ubiquitination
K513 Ubiquitination
S602 Phosphorylation
S609 Phosphorylation
K615 Ubiquitination
Y616 Phosphorylation
K716 Ubiquitination
T723 Phosphorylation
K728 Ubiquitination
K811 Ubiquitination
K851 Ubiquitination
K852 Acetylation
K857 Acetylation
K857 Ubiquitination
K860 Ubiquitination
T873 Phosphorylation
S878 Phosphorylation
Y885 Phosphorylation
S900 Phosphorylation
S919 Phosphorylation
S926 Phosphorylation
S930 Phosphorylation
S938 Phosphorylation
K940 Ubiquitination
K947 Ubiquitination
K954 Ubiquitination
S1153 Phosphorylation
K1184 Ubiquitination
K1277 Ubiquitination
K1288 Ubiquitination
S1296 Phosphorylation
S1326 Phosphorylation
T1378 Phosphorylation
S1438 Phosphorylation
S1501 Phosphorylation
S1542 Phosphorylation
K1544 Ubiquitination

研究背景

機能:

Mediates hepatobiliary excretion of numerous organic anions and conjugated organic anions such as methotrexate, 17beta-estradiol 17-glucosiduronic acid and leukotriene C4. Also transports sulfated bile salt such as taurolithocholate sulfate. May function as a cellular cisplatin transporter.

細胞の位置付け:

Apical cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed by polarized cells in liver, kidney and intestine. The highest expression is found in liver.

タンパク質ファミリー:

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.

研究領域

· Environmental Information Processing > Membrane transport > ABC transporters.

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

参考文献

1). Gut microbiota-mediated C-sulfonate metabolism impairs the bioavailability and anti-cholestatic efficacy of andrographolide. Gut Microbes, 2024 [IF=12.2]
2). 4′-OH as the Action Site of Lipids and MRP1 for Enhanced Transdermal Delivery of Flavonoids. ACS Applied Materials & Interfaces, 2023 (PubMed: 36913526) [IF=9.5]
3). Dolomiaea souliei ethyl acetate extract protected against α-naphthylisothiocyanate-induced acute intrahepatic cholestasis through regulation of farnesoid x receptor-mediated bile acid metabolism. Phytomedicine, 2021 (PubMed: 34091148) [IF=7.9]

Application: WB    Species: Rat    Sample: liver tissue

Fig. 5. Effects of DSE on expressions of bile acid metabolism and inflammation related proteins in ANIT-induced cholestasis rats. + p < 0.01, vs. control; *p < 0.05, vs. model; **p < 0.01, vs. model.
4). Advanced oxidation protein products upregulate efflux transporter expression and activity through activation of the Nrf-2-mediated signaling pathway in vitro and in vivo. European Journal of Pharmaceutical Sciences, 2020 (PubMed: 32315774) [IF=4.6]

Application: WB    Species: rat    Sample: liver

Fig. 2. |Effects of AOPPs on the expression of efflux transporter in vivo. (E) The liver tissues of sham, 5/6 nx, control and adenine groups were collected and detected by Western blot. Data are represented as the means ± SD; ***p < 0.001, **p < 0.01 and *p < 0.05 compared with the PBS group. All data were normalized with GAPDH.
5). RPF2 mediates the CARM1‑MYCN axis to promote chemotherapy resistance in colorectal cancer cells. Oncology reports, 2024 (PubMed: 37997821) [IF=4.2]
6). QiShenYiQi pills, a Chinese patent medicine, increase bioavailability of atorvastatin by inhibiting Mrp2 expression in rats. PHARMACEUTICAL BIOLOGY, 2022 (PubMed: 35001796) [IF=3.8]

Application: WB    Species: Rat    Sample: ileum

Figure 6. Protein expression of P-glycoprotein in jejunum and Mrp2 in ileum after oral or intravenous administration of ATV without or with QSYQ. (A) and (B) Protein expression of P-glycoprotein in jejunum; (C) and (D) Protein expression of Mrp2 in ileum. Data are shown as mean ± SD (n = 3). *p < 0.05 compared to without QSYQ.

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