CLIP1 Antibody - #DF3876
| 製品: | CLIP1 Antibody |
| カタログ: | DF3876 |
| タンパク質の説明: | Rabbit polyclonal antibody to CLIP1 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 161 KD; 162kD(Calculated). |
| ユニプロット: | P30622 |
| RRID: | AB_2836229 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3876, RRID:AB_2836229.
折りたたみ／展開
CAP GLY domain containing linker protein 1; CAP-Gly domain-containing linker protein 1; CLIP 170; CLIP; CLIP-170; CLIP1; CLIP1_HUMAN; CYLN1; cytoplasmic linker 1; Cytoplasmic linker protein 1; Cytoplasmic linker protein 170 alpha 2; Cytoplasmic linker protein 170 alpha-2; Cytoplasmic linker protein CLIP 170; Reed Steinberg cell expressed intermediate filament associated; Reed Sternberg intermediate filament associated protein 3; Reed-Sternberg intermediate filament-associated protein; Restin; RSN;
免疫原
Detected in dendritic cells (at protein level). Highly expressed in the Reed-Sternberg cells of Hodgkin disease.
- P30622 CLIP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSMLKPSGLKAPTKILKPGSTALKTPTAVVAPVEKTISSEKASSTPSSETQEEFVDDFRVGERVWVNGNKPGFIQFLGETQFAPGQWAGIVLDEPIGKNDGSVAGVRYFQCEPLKGIFTRPSKLTRKVQAEDEANGLQTTPASRATSPLCTSTASMVSSSPSTPSNIPQKPSQPAAKEPSATPPISNLTKTASESISNLSEAGSIKKGERELKIGDRVLVGGTKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQPKYGLFAPVHKVTKIGFPSTTPAKAKANAVRRVMATTSASLKRSPSASSLSSMSSVASSVSSRPSRTGLLTETSSRYARKISGTTALQEALKEKQQHIEQLLAERDLERAEVAKATSHVGEIEQELALARDGHDQHVLELEAKMDQLRTMVEAADREKVELLNQLEEEKRKVEDLQFRVEEESITKGDLEQKSQISEDPENTQTKLEHARIKELEQSLLFEKTKADKLQRELEDTRVATVSEKSRIMELEKDLALRVQEVAELRRRLESNKPAGDVDMSLSLLQEISSLQEKLEVTRTDHQREITSLKEHFGAREETHQKEIKALYTATEKLSKENESLKSKLEHANKENSDVIALWKSKLETAIASHQQAMEELKVSFSKGLGTETAEFAELKTQIEKMRLDYQHEIENLQNQQDSERAAHAKEMEALRAKLMKVIKEKENSLEAIRSKLDKAEDQHLVEMEDTLNKLQEAEIKVKELEVLQAKCNEQTKVIDNFTSQLKATEEKLLDLDALRKASSEGKSEMKKLRQQLEAAEKQIKHLEIEKNAESSKASSITRELQGRELKLTNLQENLSEVSQVKETLEKELQILKEKFAEASEEAVSVQRSMQETVNKLHQKEEQFNMLSSDLEKLRENLADMEAKFREKDEREEQLIKAKEKLENDIAEIMKMSGDNSSQLTKMNDELRLKERDVEELQLKLTKANENASFLQKSIEDMTVKAEQSQQEAAKKHEEEKKELERKLSDLEKKMETSHNQCQELKARYERATSETKTKHEEILQNLQKTLLDTEDKLKGAREENSGLLQELEELRKQADKAKAAQTAEDAMQIMEQMTKEKTETLASLEDTKQTNAKLQNELDTLKENNLKNVEELNKSKELLTVENQKMEEFRKEIETLKQAAAQKSQQLSALQEENVKLAEELGRSRDEVTSHQKLEEERSVLNNQLLEMKKRESKFIKDADEEKASLQKSISITSALLTEKDAELEKLRNEVTVLRGENASAKSLHSVVQTLESDKVKLELKVKNLELQLKENKRQLSSSSGNTDTQADEDERAQESQIDFLNSVIVDLQRKNQDLKMKVEMMSEAALNGNGDDLNNYDSDDQEKQSKKKPRLFCDICDCFDLHDTEDCPTQAQMSEDPPHSTHHGSRGEERPYCEICEMFGHWATNCNDDETF
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P30622 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| T25 | Phosphorylation | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| T45 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| T50 | Phosphorylation | Uniprot | |
| T139 | Phosphorylation | Uniprot | |
| T140 | Phosphorylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| T146 | Phosphorylation | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| T163 | Phosphorylation | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| T189 | Phosphorylation | Uniprot | |
| T191 | Phosphorylation | Uniprot | |
| S193 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S197 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| T260 | Phosphorylation | Uniprot | |
| Y262 | Phosphorylation | Uniprot | |
| Y269 | Phosphorylation | Uniprot | |
| T279 | Phosphorylation | Uniprot | |
| S285 | Phosphorylation | Uniprot | |
| T286 | Phosphorylation | Uniprot | |
| T287 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Q6P5Z2 (PKN3) , P17252 (PRKCA) , Q16512 (PKN1) , P53350 (PLK1) | Uniprot |
| S314 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| S328 | Phosphorylation | Uniprot | |
| T333 | Phosphorylation | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| T350 | Phosphorylation | Uniprot | |
| T351 | Phosphorylation | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| K409 | Ubiquitination | Uniprot | |
| T501 | Phosphorylation | Uniprot | |
| S510 | Phosphorylation | Uniprot | |
| K574 | Ubiquitination | Uniprot | |
| K589 | Ubiquitination | Uniprot | |
| S607 | Phosphorylation | Uniprot | |
| K647 | Ubiquitination | Uniprot | |
| K751 | Ubiquitination | Uniprot | |
| K767 | Ubiquitination | Uniprot | |
| S783 | Phosphorylation | Uniprot | |
| S784 | Phosphorylation | Uniprot | |
| S788 | Phosphorylation | Uniprot | |
| S815 | Phosphorylation | Uniprot | |
| T833 | Phosphorylation | Uniprot | |
| S840 | Phosphorylation | Uniprot | |
| S843 | Phosphorylation | Uniprot | |
| T848 | Phosphorylation | Uniprot | |
| K967 | Ubiquitination | Uniprot | |
| S973 | Phosphorylation | Uniprot | |
| K1007 | Acetylation | Uniprot | |
| S1009 | Phosphorylation | Uniprot | |
| K1013 | Acetylation | Uniprot | |
| S1066 | Phosphorylation | Uniprot | |
| T1087 | Phosphorylation | Uniprot | |
| S1140 | Phosphorylation | Uniprot | |
| S1169 | Phosphorylation | Uniprot | |
| S1230 | Phosphorylation | Uniprot | |
| S1234 | Phosphorylation | Uniprot | |
| S1236 | Phosphorylation | Uniprot | |
| S1239 | Phosphorylation | Uniprot | |
| K1245 | Ubiquitination | Uniprot | |
| S1302 | Phosphorylation | Uniprot | |
| S1303 | Phosphorylation | Uniprot | |
| S1304 | Phosphorylation | Uniprot | |
| S1305 | Phosphorylation | Uniprot | |
| T1308 | Phosphorylation | Uniprot | |
| T1310 | Phosphorylation | Uniprot | |
| S1364 | Phosphorylation | P53350 (PLK1) , P68400 (CSNK2A1) | Uniprot |
研究背景
Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking.
Phosphorylated. Phosphorylation induces conformational changes by increasing the affinity of the N-terminus for C-terminus, resulting in inhibition of its function thus decreasing its binding to microtubules and DCTN1. Exhibits a folded, autoinhibited conformation when phosphorylated and an open conformation when dephosphorylated with increased binding affinity to microtubules and DCTN1. Phosphorylation regulates its recruitment to tyrosinated microtubules and the recruitment of vesicular cargo to microtubules in neurons (By similarity). Phosphorylation by MTOR may positively regulate CLIP1 association with microtubules.
Cytoplasm. Cytoplasm>Cytoskeleton. Cytoplasmic vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Ruffle.
Note: Localizes to microtubule plus ends (PubMed:21646404, PubMed:17889670). Localizes preferentially to the ends of tyrosinated microtubules (By similarity). Accumulates in plasma membrane regions with ruffling and protrusions. Associates with the membranes of intermediate macropinocytic vesicles (PubMed:12433698).
Detected in dendritic cells (at protein level). Highly expressed in the Reed-Sternberg cells of Hodgkin disease.
Interacts with MTOR; phosphorylates and regulates CLIP1. Interacts (via CAP-Gly domains) with tubulin. Interacts with SLAIN2. Interacts (via zinc finger) with DCTN1. Interacts with TUBA1B, MAPRE1 and MAPRE3. Binds preferentially to tyrosinated microtubules, and only marginally to detyrosinated microtubules (By similarity).
Intramolecular interaction between the zinc finger domain and the CAP-Gly domains may inhibit interaction with tubulin.
研究領域
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
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