CHST2 Antibody - #DF3883
| 製品: | CHST2 Antibody |
| カタログ: | DF3883 |
| タンパク質の説明: | Rabbit polyclonal antibody to CHST2 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human |
| 予測: | Bovine, Dog |
| 分子量: | 58 KD; 58kD(Calculated). |
| ユニプロット: | Q9Y4C5 |
| RRID: | AB_2836236 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3883, RRID:AB_2836236.
折りたたみ／展開
AI428561; AW121776; C130041E03Rik; C6ST; Carbohydrate (chondroitin 6/keratan) sulfotransferase 2; Carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2; Carbohydrate sulfotransferase 2; CHST2; CHST2_HUMAN; Chts2; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2; GlcNAc6ST; GlcNAc6ST-1; Gn6st; Gn6st-1; GST 2; GST-2; GST2; N-acetylglucosamine 6-O-sulfotransferase 1; N-acetylglucosamine 6-O-sulfotransferase;
免疫原
Widely expressed. Highly expressed in bone marrow, peripheral blood leukocytes, spleen, brain, spinal cord, ovary and placenta. Expressed by high endothelial cells (HEVs) and leukocytes.
- Q9Y4C5 CHST2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRSPQRALPPGALPRLLQAAPAAAPRALLPQWPRRPGRRWPASPLGMKVFRRKALVLCAGYALLLVLTMLNLLDYKWHKEPLQQCNPDGPLGAAAGAAGGSWGRPGPPPAGPPRAHARLDLRTPYRPPAAAVGAAPAAAAGMAGVAAPPGNGTRGTGGVGDKRQLVYVFTTWRSGSSFFGELFNQNPEVFFLYEPVWHVWQKLYPGDAVSLQGAARDMLSALYRCDLSVFQLYSPAGSGGRNLTTLGIFGAATNKVVCSSPLCPAYRKEVVGLVDDRVCKKCPPQRLARFEEECRKYRTLVIKGVRVFDVAVLAPLLRDPALDLKVIHLVRDPRAVASSRIRSRHGLIRESLQVVRSRDPRAHRMPFLEAAGHKLGAKKEGVGGPADYHALGAMEVICNSMAKTLQTALQPPDWLQGHYLVVRYEDLVGDPVKTLRRVYDFVGLLVSPEMEQFALNMTSGSGSSSKPFVVSARNATQAANAWRTALTFQQIKQVEEFCYQPMAVLGYERVNSPEEVKDLSKTLLRKPRL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y4C5 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N243 | N-Glycosylation | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| Y425 | Phosphorylation | Uniprot | |
| N457 | N-Glycosylation | Uniprot | |
| N475 | N-Glycosylation | Uniprot | |
| K518 | Ubiquitination | Uniprot |
研究背景
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains.
Glycosylation at Asn-475 is required for catalytic activity.
Golgi apparatus>trans-Golgi network membrane>Single-pass type II membrane protein.
Widely expressed. Highly expressed in bone marrow, peripheral blood leukocytes, spleen, brain, spinal cord, ovary and placenta. Expressed by high endothelial cells (HEVs) and leukocytes.
Homodimer; disulfide-linked. Homodimerization is not essential for enzyme activity.
Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
研究領域
· Metabolism > Glycan biosynthesis and metabolism > Glycosaminoglycan biosynthesis - keratan sulfate.
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