CLN5 Antibody - #DF3927
| 製品: | CLN5 Antibody |
| カタログ: | DF3927 |
| タンパク質の説明: | Rabbit polyclonal antibody to CLN5 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 46 KD; 41kD(Calculated). |
| ユニプロット: | O75503 |
| RRID: | AB_2836280 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3927, RRID:AB_2836280.
折りたたみ／展開
Ceroid lipofuscinosis neuronal 5; Ceroid-lipofuscinosis neuronal protein 5; CLN5; CLN5_HUMAN; NCL; Protein CLN5;
免疫原
- O75503 CLN5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQEVDTAQGAEMRRGAGAARGRASWCWALALLWLAVVPGWSRVSGIPSRRHWPVPYKRFDFRPKPDPYCQAKYTFCPTGSPIPVMEGDDDIEVFRLQAPVWEFKYGDLLGHLKIMHDAIGFRSTLTGKNYTMEWYELFQLGNCTFPHLRPEMDAPFWCNQGAACFFEGIDDVHWKENGTLVQVATISGNMFNQMAKWVKQDNETGIYYETWNVKASPEKGAETWFDSYDCSKFVLRTFNKLAEFGAEFKNIETNYTRIFLYSGEPTYLGNETSVFGPTGNKTLGLAIKRFYYPFKPHLPTKEFLLSLLQIFDAVIVHKQFYLFYNFEYWFLPMKFPFIKITYEEIPLPIRNKTLSGL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O75503 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K65 | Ubiquitination | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| N130 | N-Glycosylation | Uniprot | |
| N143 | N-Glycosylation | Uniprot | |
| N178 | N-Glycosylation | Uniprot | |
| N203 | N-Glycosylation | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| N255 | N-Glycosylation | Uniprot | |
| N271 | N-Glycosylation | Uniprot | |
| N281 | N-Glycosylation | Uniprot | |
| T283 | Phosphorylation | Uniprot | |
| N352 | N-Glycosylation | Uniprot |
研究背景
Plays a role in influencing the retrograde trafficking of lysosomal sorting receptors SORT1 and IGF2R from the endosomes to the trans-Golgi network by controlling the recruitment of retromer complex to the endosomal membrane. Regulates the localization and activation of RAB7A which is required to recruit the retromer complex to the endosomal membrane.
N-glycosylated with both high mannose and complex type sugars. Glycosylation is important for proper folding and trafficking to the lysosomes.
The type II membrane signal anchor is proteolytically cleaved to produce a mature form that is transported to the lysosomes (Ceroid-lipofuscinosis neuronal protein 5, secreted form).
Can undergo proteolytic cleavage at the C-terminus, probably by a cysteine protease and may involve the removal of approximately 10-15 residues from the C-terminal end.
Lysosome.
Membrane>Single-pass type II membrane protein.
Note: An amphipathic anchor region facilitates its association with the membrane.
Ubiquitous.
Interacts with SORT1, RAB5A and RAB7A. Interacts with PPT1, TPP1, CLN3, CLN6, CLN8, ATP5F1A and ATP5F1B (By similarity).
Belongs to the CLN5 family.
研究領域
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
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