C9 Antibody - #DF3964
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3964, RRID:AB_2836317.
折りたたみ／展開
C9; C9 deficiency; C9 deficiency with dermatomyositis; CO9_HUMAN; Complement component 9; Complement component 9 deficiency; Complement component C9; Complement component C9b;
免疫原
- P02748 CO9_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSACRSFAVAICILEISILTAQYTTSYDPELTESSGSASHIDCRMSPWSEWSQCDPCLRQMFRSRSIEVFGQFNGKRCTDAVGDRRQCVPTEPCEDAEDDCGNDFQCSTGRCIKMRLRCNGDNDCGDFSDEDDCESEPRPPCRDRVVEESELARTAGYGINILGMDPLSTPFDNEFYNGLCNRDRDGNTLTYYRRPWNVASLIYETKGEKNFRTEHYEEQIEAFKSIIQEKTSNFNAAISLKFTPTETNKAEQCCEETASSISLHGKGSFRFSYSKNETYQLFLSYSSKKEKMFLHVKGEIHLGRFVMRNRDVVLTTTFVDDIKALPTTYEKGEYFAFLETYGTHYSSSGSLGGLYELIYVLDKASMKRKGVELKDIKRCLGYHLDVSLAFSEISVGAEFNKDDCVKRGEGRAVNITSENLIDDVVSLIRGGTRKYAFELKEKLLRGTVIDVTDFVNWASSINDAPVLISQKLSPIYNLVPVKMKNAHLKKQNLERAIEDYINEFSVRKCHTCQNGGTVILMDGKCLCACPFKFEGIACEISKQKISEGLPALEFPNEK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P02748 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| W48 | C-Glycosylation | Uniprot | |
| W51 | C-Glycosylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| K225 | Ubiquitination | Uniprot | |
| T244 | Phosphorylation | Uniprot | |
| T248 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| N277 | N-Glycosylation | Uniprot | |
| T316 | Phosphorylation | Uniprot | |
| Y383 | Phosphorylation | Uniprot | |
| S392 | Phosphorylation | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| N415 | N-Glycosylation | Uniprot | |
| K435 | Ubiquitination | Uniprot |
研究背景
Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.
Thrombin cleaves factor C9 to produce C9a and C9b.
Phosphorylation sites are present in the extracellular medium.
Initially, positions and connectivity of disulfide bonds were based on peptide sequencing done for the human protein. The crystal structures for the human and mouse proteins corrected the positions and connectivities of the disulfide bonds. The distance between Cys-57 and Cys-94 in the monomeric mouse protein precludes formation of a disulfide bond, contrary to what is seen in the structure of the human polymeric form of the protein (Probable).
Secreted. Target cell membrane>Multi-pass membrane protein.
Note: Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.
Plasma (at protein level).
Component of the membrane attack complex (MAC). MAC assembly is initiated by proteolytic cleavage of C5 into C5a and C5b. C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9. About 20 C9 chains oligomerize to give rise to a huge beta-barrel that forms a 100 Angstrom diameter pore in target membranes.
Belongs to the complement C6/C7/C8/C9 family.
研究領域
· Human Diseases > Neurodegenerative diseases > Prion diseases.
· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.
· Human Diseases > Immune diseases > Systemic lupus erythematosus.
· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.