Factor I/CFI Antibody - #DF3966
| 製品: | Factor I/CFI Antibody |
| カタログ: | DF3966 |
| タンパク質の説明: | Rabbit polyclonal antibody to Factor I/CFI |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human |
| 予測: | Pig, Zebrafish, Bovine, Dog |
| 分子量: | 66 KD; 66kD(Calculated). |
| ユニプロット: | P05156 |
| RRID: | AB_2836319 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3966, RRID:AB_2836319.
折りたたみ／展開
AHUS3; ARMD13; C3b INA; C3b inactivator; C3B/C4B inactivator; C3BINA; CFAI_HUMAN; Cfi; Complement component I; Complement control protein factor I; Complement factor I; Complement factor I heavy chain; Complement factor I light chain; F1; factor I; FactorI; FI; I factor; IF; KAF; Konglutinogen activating factor; Light chain of factor I; OTTHUMP00000219728; OTTHUMP00000221928;
免疫原
Expressed in the liver by hepatocytes (PubMed:6327681). Also present in other cells such as monocytes, fibroblasts or keratinocytes (PubMed:6444659, PubMed:17320177).
- P05156 CFAI_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKLLHVFLLFLCFHLRFCKVTYTSQEDLVEKKCLAKKYTHLSCDKVFCQPWQRCIEGTCVCKLPYQCPKNGTAVCATNRRSFPTYCQQKSLECLHPGTKFLNNGTCTAEGKFSVSLKHGNTDSEGIVEVKLVDQDKTMFICKSSWSMREANVACLDLGFQQGADTQRRFKLSDLSINSTECLHVHCRGLETSLAECTFTKRRTMGYQDFADVVCYTQKADSPMDDFFQCVNGKYISQMKACDGINDCGDQSDELCCKACQGKGFHCKSGVCIPSQYQCNGEVDCITGEDEVGCAGFASVTQEETEILTADMDAERRRIKSLLPKLSCGVKNRMHIRRKRIVGGKRAQLGDLPWQVAIKDASGITCGGIYIGGCWILTAAHCLRASKTHRYQIWTTVVDWIHPDLKRIVIEYVDRIIFHENYNAGTYQNDIALIEMKKDGNKKDCELPRSIPACVPWSPYLFQPNDTCIVSGWGREKDNERVFSLQWGEVKLISNCSKFYGNRFYEKEMECAGTYDGSIDACKGDSGGPLVCMDANNVTYVWGVVSWGENCGKPEFPGVYTKVANYFDWISYHVGRPFISQYNV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P05156 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y22 | Phosphorylation | Uniprot | |
| N70 | N-Glycosylation | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| N103 | N-Glycosylation | Uniprot | |
| N177 | N-Glycosylation | Uniprot | |
| Y206 | Phosphorylation | Uniprot | |
| T425 | Phosphorylation | Uniprot | |
| N464 | N-Glycosylation | Uniprot | |
| S483 | Phosphorylation | Uniprot | |
| N494 | N-Glycosylation | Uniprot | |
| N536 | N-Glycosylation | Uniprot |
研究背景
Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways. Inhibits these pathways by cleaving three peptide bonds in the alpha-chain of C3b and two bonds in the alpha-chain of C4b thereby inactivating these proteins. Essential cofactors for these reactions include factor H and C4BP in the fluid phase and membrane cofactor protein/CD46 and CR1 on cell surfaces. The presence of these cofactors on healthy cells allows degradation of deposited C3b by CFI in order to prevent undesired complement activation, while in apoptotic cells or microbes, the absence of such cofactors leads to C3b-mediated complement activation and subsequent opsonization.
Secreted>Extracellular space. Secreted.
Expressed in the liver by hepatocytes. Also present in other cells such as monocytes, fibroblasts or keratinocytes.
Heterodimer of a light and heavy chains; disulfide-linked. The fully processed and mature protein circulates as a zymogen, and is allosterically activated by substrate-induced remodeling of the active site. Interacts with C3b. Interacts with complement factor H.
(Microbial infection) Interacts with Staphylococcus aureus clumping factor A/ClfA; this interaction enhances cleavage of C3b into iC3b by CFI.
Belongs to the peptidase S1 family.
研究領域
· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.
· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)
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