ELOVL4 Antibody - #DF4037

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製品: ELOVL4 Antibody
カタログ: DF4037
タンパク質の説明: Rabbit polyclonal antibody to ELOVL4
アプリケーション: WB IF/ICC
反応性: Human, Mouse
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 37 KD; 37kD(Calculated).
ユニプロット: Q9GZR5
RRID: AB_2836330

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MS Report
HPLC Report
MSDS
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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%)
クローナリティ:
Polyclonal
特異性:
ELOVL4 Antibody detects endogenous levels of total ELOVL4.
RRID:
AB_2836330
引用形式: Affinity Biosciences Cat# DF4037, RRID:AB_2836330.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

3-keto acyl-CoA synthase ELOVL4; ADMD; Cancer/testis antigen 118; CT118; Elongation of very long chain fatty acids (FEN1/Elo2 SUR4/Elo3 yeast) like 4; Elongation of very long chain fatty acids like 4; Elongation of very long chain fatty acids protein 4; ELOV L4; ELOV4_HUMAN; ELOVL 4; ELOVL4; FLJ17667; FLJ92876; Stargardt disease 3; Stargardt disease 3 autosomal dominant; STGD 2; STGD 3; STGD2; STGD3;

免疫原

免疫原:
Uniprot:

Q9GZR5(ELOV4_HUMAN) >>Visit HPA database.

遺伝子(ID):
ELOVL4(6785)
発現特異性:
Q9GZR5 ELOV4_HUMAN:

Expressed in the retina and at much lower level in the brain. Ubiquitous, highest expression in thymus, followed by testis, small intestine, ovary, and prostate. Little or no expression in heart, lung, liver, or leukocates.

タンパク質配列:
MGLLDSEPGSVLNVVSTALNDTVEFYRWTWSIADKRVENWPLMQSPWPTLSISTLYLLFVWLGPKWMKDREPFQMRLVLIIYNFGMVLLNLFIFRELFMGSYNAGYSYICQSVDYSNNVHEVRIAAALWWYFVSKGVEYLDTVFFILRKKNNQVSFLHVYHHCTMFTLWWIGIKWVAGGQAFFGAQLNSFIHVIMYSYYGLTAFGPWIQKYLWWKRYLTMLQLIQFHVTIGHTALSLYTDCPFPKWMHWALIAYAISFIFLFLNFYIRTYKEPKKPKAGKTAMNGISANGVSKSEKQLMIENGKKQKNGKAKGD

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
80
Xenopus
60
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9GZR5 基板として

Site PTM Type Enzyme
N20 N-Glycosylation
K35 Ubiquitination
K280 Ubiquitination
S292 Phosphorylation
S294 Phosphorylation
K296 Ubiquitination

研究背景

機能:

Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that catalyzes the synthesis of very long chain saturated (VLC-SFA) and polyunsaturated (PUFA) fatty acids that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May play a critical role in early brain and skin development.

PTMs:

N-glycosylated.

細胞の位置付け:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in the retina and at much lower level in the brain. Ubiquitous, highest expression in thymus, followed by testis, small intestine, ovary, and prostate. Little or no expression in heart, lung, liver, or leukocates.

サブユニット構造:

Oligomer.

タンパク質ファミリー:

The C-terminal di-lysine motif confers endoplasmic reticulum localization.

Belongs to the ELO family. ELOVL4 subfamily.

研究領域

· Metabolism > Lipid metabolism > Fatty acid elongation.

参考文献

1). Horse-Derived Ceramide Accentuates Glucosylceramide Synthase and Ceramide Synthase 3 by Activating PPARβ/δ and/or PPARγ to Stimulate Ceramide Synthesis. Biomedicines, 2023 (PubMed: 36831084) [IF=4.7]

Application: WB    Species: Human    Sample: RHEEs

Figure 4. Effect of HC on expression levels of ceramide synthesis- and keratinization-related proteins in RHEEs. (a) ELOVL4, (b) CERS3, (c) GCS, (d) GBA, (e) SMS1, (f) ASM, (g) INV, (h) TGM1, (i) TGM3. RHEEs were treated with HC (at 0, 10 and 20 mg/mL) for 4 days and total proteins were extracted. Protein expression levels were determined by Western blotting as described in the Section 4 Materials and Methods and are expressed as each protein level/β-actin. Bars represent means ± SD, n = 3, Dunnett’s multiple comparison test.

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