製品: GNPAT Antibody
カタログ: DF3993
タンパク質の説明: Rabbit polyclonal antibody to GNPAT
アプリケーション: WB IF/ICC
反応性: Human
予測: Rat, Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 77 KD; 77kD(Calculated).
ユニプロット: O15228
RRID: AB_2836353

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Rat(%), Pig(100%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
GNPAT Antibody detects endogenous levels of total GNPAT.
RRID:
AB_2836353
引用形式: Affinity Biosciences Cat# DF3993, RRID:AB_2836353.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Acyl CoA dihydroxyacetonephosphateacyltransferase; Acyl-CoA:dihydroxyacetonephosphateacyltransferase; DAP AT; DAP-AT; DAPAT; DHAP AT; DHAP-AT; DHAPAT; Dihydroxyacetone phosphate acyltransferase; EC 2.3.1.42; Glycerone-phosphate O-acyltransferase; Glyceronephosphate O acyltransferase; GNPAT; GNPAT_HUMAN; OTTHUMP00000036147;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MESSSSSNSYFSVGPTSPSAVVLLYSKELKKWDEFEDILEERRHVSDLKFAMKCYTPLVYKGITPCKPIDIKCSVLNSEEIHYVIKQLSKESLQSVDVLREEVSEILDEMSHKLRLGAIRFCAFTLSKVFKQIFSKVCVNEEGIQKLQRAIQEHPVVLLPSHRSYIDFLMLSFLLYNYDLPVPVIAAGMDFLGMKMVGELLRMSGAFFMRRTFGGNKLYWAVFSEYVKTMLRNGYAPVEFFLEGTRSRSAKTLTPKFGLLNIVMEPFFKREVFDTYLVPISISYDKILEETLYVYELLGVPKPKESTTGLLKARKILSENFGSIHVYFGDPVSLRSLAAGRMSRSSYNLVPRYIPQKQSEDMHAFVTEVAYKMELLQIENMVLSPWTLIVAVLLQNRPSMDFDALVEKTLWLKGLTQAFGGFLIWPDNKPAEEVVPASILLHSNIASLVKDQVILKVDSGDSEVVDGLMLQHITLLMCSAYRNQLLNIFVRPSLVAVALQMTPGFRKEDVYSCFRFLRDVFADEFIFLPGNTLKDFEEGCYLLCKSEAIQVTTKDILVTEKGNTVLEFLVGLFKPFVESYQIICKYLLSEEEDHFSEEQYLAAVRKFTSQLLDQGTSQCYDVLSSDVQKNALAACVRLGVVEKKKINNNCIFNVNEPATTKLEEMLGCKTPIGKPATAKL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Bovine
91
Sheep
91
Zebrafish
64
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15228 基板として

Site PTM Type Enzyme
S17 Phosphorylation
K31 Ubiquitination
K49 Ubiquitination
K67 Ubiquitination
K90 Ubiquitination
S111 Phosphorylation
K113 Ubiquitination
K128 Acetylation
K136 Ubiquitination
K146 Ubiquitination
K251 Ubiquitination
T291 Phosphorylation
K304 Ubiquitination
K312 Ubiquitination
S345 Phosphorylation
S346 Phosphorylation
S589 Phosphorylation
K606 Ubiquitination
S625 Phosphorylation
K643 Acetylation
K644 Acetylation
K674 Ubiquitination

研究背景

細胞の位置付け:

Peroxisome membrane>Peripheral membrane protein>Matrix side.
Note: Exclusively localized to the lumenal side of the peroxisomal membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

May be part of a heterotrimeric complex composed of DAP-AT, ADAP-S and a modified form of DAP-AT.

タンパク質ファミリー:

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.

Belongs to the GPAT/DAPAT family.

研究領域

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

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