RPA2 Antibody - #DF4001
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4001, RRID:AB_2836361.
折りたたみ／展開
A127; DNA directed RNA polymerase I 135kDa polypeptide; DNA directed RNA polymerase I; DNA directed RNA polymerase I subunit RPA2; DNA-directed RNA polymerase I 135 kDa polypeptide; DNA-directed RNA polymerase I subunit rpa2; FLJ10816; FLJ21921; MGC131780; OTTHUMP00000161989; OTTHUMP00000203643; OTTHUMP00000203645; POLR 1B; polr1b; Polymerase (RNA) I polypeptide B 128kDa; Polymerase (RNA) I polypeptide B; RNA polymerase I polypeptide B; RNA polymerase I subunit 2; RPA 135; RPA116; RPA135; RPA2; RPA2_HUMAN; Rpo1 2;
免疫原
- Q9H9Y6 RPA2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDPGSRWRNLPSGPSLKHLTDPSYGIPREQQKAALQELTRAHVESFNYAVHEGLGLAVQAIPPFEFAFKDERISFTILDAVISPPTVPKGTICKEANVYPAECRGRRSTYRGKLTADINWAVNGISKGIIKQFLGYVPIMVKSKLCNLRNLPPQALIEHHEEAEEMGGYFIINGIEKVIRMLIMPRRNFPIAMIRPKWKTRGPGYTQYGVSMHCVREEHSAVNMNLHYLENGTVMLNFIYRKELFFLPLGFALKALVSFSDYQIFQELIKGKEDDSFLRNSVSQMLRIVMEEGCSTQKQVLNYLGECFRVKLNVPDWYPNEQAAEFLFNQCICIHLKSNTEKFYMLCLMTRKLFALAKGECMEDNPDSLVNQEVLTPGQLFLMFLKEKLEGWLVSIKIAFDKKAQKTSVSMNTDNLMRIFTMGIDLTKPFEYLFATGNLRSKTGLGLLQDSGLCVVADKLNFIRYLSHFRCVHRGADFAKMRTTTVRRLLPESWGFLCPVHTPDGEPCGLMNHLTAVCEVVTQFVYTASIPALLCNLGVTPIDGAPHRSYSECYPVLLDGVMVGWVDKDLAPGIADSLRHFKVLREKRIPPWMEVVLIPMTGKPSLYPGLFLFTTPCRLVRPVQNLALGKEELIGTMEQIFMNVAIFEDEVFAGVTTHQELFPHSLLSVIANFIPFSDHNQSPRNMYQCQMGKQTMGFPLLTYQDRSDNKLYRLQTPQSPLVRPSMYDYYDMDNYPIGTNAIVAVISYTGYDMEDAMIVNKASWERGFAHGSVYKSEFIDLSEKIKQGDSSLVFGIKPGDPRVLQKLDDDGLPFIGAKLQYGDPYYSYLNLNTGESFVMYYKSKENCVVDNIKVCSNDTGSGKFKCVCITMRVPRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMVPDILFNPHGFPSRMTIGMLIESMAGKSAALHGLCHDATPFIFSEENSALEYFGEMLKAAGYNFYGTERLYSGISGLELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDRVTNQPIGGRNVQGGIRFGEMERDALLAHGTSFLLHDRLFNCSDRSVAHVCVKCGSLLSPLLEKPPPSWSAMRNRKYNCTLCSRSDTIDTVSVPYVFRYFVAELAAMNIKVKLDVV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H9Y6 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K17 | Methylation | Uniprot | |
| K17 | Ubiquitination | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| Y136 | Phosphorylation | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| K298 | Ubiquitination | Uniprot | |
| K406 | Ubiquitination | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| K582 | Ubiquitination | Uniprot | |
| K693 | Methylation | Uniprot | |
| K693 | Ubiquitination | Uniprot | |
| K784 | Ubiquitination | Uniprot | |
| K786 | Ubiquitination | Uniprot | |
| K797 | Ubiquitination | Uniprot | |
| K806 | Ubiquitination | Uniprot | |
| K844 | Ubiquitination | Uniprot | |
| K853 | Ubiquitination | Uniprot | |
| K863 | Methylation | Uniprot | |
| K863 | Ubiquitination | Uniprot | |
| K882 | Ubiquitination | Uniprot | |
| Y971 | Phosphorylation | Uniprot | |
| Y998 | Phosphorylation | Uniprot | |
| Y999 | Phosphorylation | Uniprot | |
| K1009 | Ubiquitination | Uniprot | |
| S1051 | Phosphorylation | Uniprot | |
| K1083 | Ubiquitination | Uniprot | |
| K1095 | Ubiquitination | Uniprot | |
| Y1118 | Phosphorylation | Uniprot |
研究背景
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).
Nucleus>Nucleolus.
Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits.
Belongs to the RNA polymerase beta chain family.
研究領域
· Genetic Information Processing > Transcription > RNA polymerase.
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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