CD3EAP Antibody - #DF4002
| 製品: | CD3EAP Antibody |
| カタログ: | DF4002 |
| タンパク質の説明: | Rabbit polyclonal antibody to CD3EAP |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human |
| 分子量: | 55 KD; 55kD(Calculated). |
| ユニプロット: | O15446 |
| RRID: | AB_2836362 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4002, RRID:AB_2836362.
折りたたみ／展開
A34.5; Anti sense to ERCC 1 protein; Antisense to ERCC-1 protein; ASE 1; ASE-1; ASE1; CAST; CD3 epsilon associated protein; CD3-epsilon-associated protein; CD3E antigen, epsilon polypeptide associated protein; CD3E associated protein; CD3e molecule, epsilon associated protein; CD3E-associated protein; CD3EAP; DNA directed RNA polymerase I subunit RPA34; DNA-directed RNA polymerase I subunit RPA34; MGC118851; PAF 49; RNA polymerase I associated factor PAF49; RNA polymerase I-associated factor PAF49; RPA34_HUMAN;
免疫原
- O15446 RPA34_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEEPQAGDAARFSCPPNFTAKPPASESPRFSLEALTGPDTELWLIQAPADFAPECFNGRHVPLSGSQIVKGKLAGKRHRYRVLSSCPQAGEATLLAPSTEAGGGLTCASAPQGTLRILEGPQQSLSGSPLQPIPASPPPQIPPGLRPRFCAFGGNPPVTGPRSALAPNLLTSGKKKKEMQVTEAPVTQEAVNGHGALEVDMALGSPEMDVRKKKKKKNQQLKEPEAAGPVGTEPTVETLEPLGVLFPSTTKKRKKPKGKETFEPEDKTVKQEQINTEPLEDTVLSPTKKRKRQKGTEGMEPEEGVTVESQPQVKVEPLEEAIPLPPTKKRKKEKGQMAMMEPGTEAMEPVEPEMKPLESPGGTMAPQQPEGAKPQAQAALAAPKKKTKKEKQQDATVEPETEVVGPELPDDLEPQAAPTSTKKKKKKKERGHTVTEPIQPLEPELPGEGQPEARATPGSTKKRKKQSQESRMPETVPQEEMPGPPLNSESGEEAPTGRDKKRKQQQQQPV
PTMs - O15446 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| Y80 | Phosphorylation | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| S126 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| C150 | S-Nitrosylation | Uniprot | |
| T159 | Phosphorylation | Uniprot | |
| R162 | Methylation | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| T171 | Phosphorylation | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| K174 | Acetylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| T249 | Phosphorylation | Uniprot | |
| K270 | Sumoylation | Uniprot | |
| T276 | Phosphorylation | Uniprot | |
| T282 | Phosphorylation | Uniprot | |
| S285 | Phosphorylation | P06493 (CDK1) | Uniprot |
| T287 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| K314 | Sumoylation | Uniprot | |
| S359 | Phosphorylation | Uniprot | |
| T363 | Phosphorylation | Uniprot | |
| T419 | Phosphorylation | Uniprot | |
| T421 | Phosphorylation | Uniprot | |
| T456 | Phosphorylation | Uniprot | |
| S459 | Phosphorylation | Uniprot | |
| T475 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| S490 | Phosphorylation | Uniprot |
研究背景
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.
Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.
Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other groups.
Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes.
Nucleus>Nucleolus. Chromosome.
Note: Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes.
Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1A thereby associates with the SL1 complex. Interacts with UBTF. Interacts with POLR1E/PRAF1 through its N-terminal region (By similarity). Isoform 2 interacts with CD3E.
Belongs to the eukaryotic RPA34 RNA polymerase subunit family.
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