RPC4 Antibody - #DF4006
| 製品: | RPC4 Antibody |
| カタログ: | DF4006 |
| タンパク質の説明: | Rabbit polyclonal antibody to RPC4 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Sheep, Rabbit, Dog |
| 分子量: | 38 KD; 44kD(Calculated). |
| ユニプロット: | P05423 |
| RRID: | AB_2836366 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4006, RRID:AB_2836366.
折りたたみ／展開
AI326118; AW489084; BN51 (BHK21) temperature sensitivity complementing; BN51; BN51T; DNA directed RNA polymerase III 47 kDa polypeptide; DNA directed RNA polymerase III subunit D; POLR3D; Polymerase (RNA) III (DNA directed) polypeptide D; Polymerase (RNA) III (DNA directed) polypeptide D, 44kDa; Protein BN51; RNA polymerase III subunit C4; RPC4; RPC53; Temperature sensitive complementation cell cycle specific tsBN51; TSBN51;
免疫原
- P05423 RPC4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSEGNAAGEPSTPGGPRPLLTGARGLIGRRPAPPLTPGRLPSIRSRDLTLGGVKKKTFTPNIISRKIKEEPKEEVTVKKEKRERDRDRQREGHGRGRGRPEVIQSHSIFEQGPAEMMKKKGNWDKTVDVSDMGPSHIINIKKEKRETDEETKQILRMLEKDDFLDDPGLRNDTRNMPVQLPLAHSGWLFKEENDEPDVKPWLAGPKEEDMEVDIPAVKVKEEPRDEEEEAKMKAPPKAARKTPGLPKDVSVAELLRELSLTKEEELLFLQLPDTLPGQPPTQDIKPIKTEVQGEDGQVVLIKQEKDREAKLAENACTLADLTEGQVGKLLIRKSGRVQLLLGKVTLDVTMGTACSFLQELVSVGLGDSRTGEMTVLGHVKHKLVCSPDFESLLDHKHR
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P05423 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| R17 | Methylation | Uniprot | |
| T21 | Phosphorylation | Uniprot | |
| R24 | Methylation | Uniprot | |
| R29 | Methylation | Uniprot | |
| R30 | Methylation | Uniprot | |
| T36 | Phosphorylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| T59 | Phosphorylation | Uniprot | |
| K78 | Sumoylation | Uniprot | |
| R90 | Methylation | Uniprot | |
| R95 | Methylation | Uniprot | |
| R97 | Methylation | Uniprot | |
| R99 | Methylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| K141 | Sumoylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| K190 | Sumoylation | Uniprot | |
| K199 | Sumoylation | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| K206 | Sumoylation | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| K220 | Sumoylation | Uniprot | |
| K247 | Ubiquitination | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| K285 | Sumoylation | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| K288 | Sumoylation | Uniprot | |
| K302 | Sumoylation | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| K310 | Ubiquitination | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| K380 | Ubiquitination | Uniprot | |
| K382 | Ubiquitination | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| K396 | Ubiquitination | Uniprot |
研究背景
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).
Nucleus.
Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). Interacts with POLR3E/RPC5.
Belongs to the eukaryotic RPC4/POLR3D RNA polymerase subunit family.
研究領域
· Genetic Information Processing > Transcription > RNA polymerase.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway. (View pathway)
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