DNAJB11 Antibody - #DF4010
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4010, RRID:AB_2836370.
折りたたみ／展開
ABBP-2; ABBP2; APOBEC1 binding protein 2; APOBEC1-binding protein 2; DJ9; DJB11_HUMAN; DnaJ (Hsp40) homolog subfamily B member 11; DnaJ homolog subfamily B member 11; DnaJ protein homolog 9; DNAJB11; EDJ; ER associated DNAJ; ER associated dnaJ protein 3; ER associated Hsp40 co chaperone; ER-associated DNAJ; ER-associated dnaJ protein 3; ER-associated Hsp40 co-chaperone; ERdj3; ERJ3; ERj3p; hDj-9; hDj9; HEDJ; Human DnaJ protein 9; PWP1 interacting protein 4; PWP1-interacting protein 4;
免疫原
- Q9UBS4 DJB11_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAPQNLSTFCLLLLYLIGAVIAGRDFYKILGVPRSASIKDIKKAYRKLALQLHPDRNPDDPQAQEKFQDLGAAYEVLSDSEKRKQYDTYGEEGLKDGHQSSHGDIFSHFFGDFGFMFGGTPRQQDRNIPRGSDIIVDLEVTLEEVYAGNFVEVVRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEVEIEPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHPIFERRGDDLYTNVTISLVESLVGFEMDITHLDGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQLTEEAREGIKQLLKQGSVQKVYNGLQGY
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UBS4 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K28 | Ubiquitination | Uniprot | |
| K43 | Acetylation | Uniprot | |
| K47 | Acetylation | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| R56 | Methylation | Uniprot | |
| K66 | Acetylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| T88 | Phosphorylation | Uniprot | |
| T188 | Phosphorylation | Uniprot | |
| T207 | Phosphorylation | Uniprot | |
| K247 | Ubiquitination | Uniprot | |
| N261 | N-Glycosylation | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| T332 | Phosphorylation | Uniprot | |
| K344 | Acetylation | Uniprot | |
| K344 | Ubiquitination | Uniprot |
研究背景
As a co-chaperone for HSPA5 it is required for proper folding, trafficking or degradation of proteins. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity. It is necessary for maturation and correct trafficking of PKD1.
Contains high-mannose Endo H-sensitive carbohydrates.
Cys-169, Cys-171, Cys-193 and Cys-196 form intramolecular disulfide bonds. The preferential partner for each Cys is not known.
Thr-188 was reported to be phosphorylated upon DNA damage by ATM or ATR; however as this position has been shown to be in the ER lumen, the in vivo relevance is not proven.
Endoplasmic reticulum lumen.
Note: Associated with the ER membrane in a C-terminally epitope-tagged construct.
Widely expressed.
Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Binds to denatured substrates in an ATP-independent manner. Interacts via the J domain with HSPA5 in an ATP-dependent manner.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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