DNAJC5 Antibody - #DF4011
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4011, RRID:AB_2836371.
折りたたみ／展開
CLN 4; CLN4; CLN4B; CSP; cysteine string protein alpha; Cysteine string protein; DJC5; DnaJ (Hsp40) homolog subfamily C member 5; DnaJ homolog subfamily C member 5; DNAJC 5; Dnajc5; DNAJC5A; DNJC5_HUMAN; NCL;
免疫原
Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.
- Q9H3Z4 DNJC5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADQRQRSLSTSGESLYHVLGLDKNATSDDIKKSYRKLALKYHPDKNPDNPEAADKFKEINNAHAILTDATKRNIYDKYGSLGLYVAEQFGEENVNTYFVLSSWWAKALFVFCGLLTCCYCCCCLCCCFNCCCGKCKPKAPEGEETEFYVSPEDLEAQLQSDEREATDTPIVIQPASATETTQLTADSHPSYHTDGFN
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H3Z4 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| S10 | Phosphorylation | P31749 (AKT1) , P17612 (PRKACA) | Uniprot |
| T11 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| Y17 | Phosphorylation | Uniprot | |
| K24 | Ubiquitination | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K41 | Acetylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| Y42 | Phosphorylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| K56 | Acetylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| Y149 | Phosphorylation | Uniprot | |
| Y192 | Phosphorylation | Uniprot |
研究背景
Acts as a general chaperone in regulated exocytosis (By similarity). Acts as a co-chaperone for the SNARE protein SNAP-25 (By similarity). Involved in the calcium-mediated control of a late stage of exocytosis (By similarity). May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings (By similarity).
Fatty acylated (By similarity). Heavily palmitoylated in the cysteine string motif (By similarity).
Ser-10 phosphorylation induces an order-to-disorder transition triggering the interaction with Lys-58. This conformational switch modulates DNAJC5's cellular functions by reducing binding to syntaxin and synaptogamin without altering HSC70 interactions.
Membrane>Lipid-anchor. Melanosome. Cell membrane.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.
Oligomers. Homodimer (By similarity). Interacts with the chaperone complex consisting of HSC70 and SGTA (By similarity). Interacts with ZDHHC13 (via ANK repeats) (By similarity). Interacts with ZDHHC17 (via ANK repeats). Interacts with SYT1, SYT5 and SYT7, and with SYT9, forming a complex with SNAP25 (By similarity).
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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