RNF138 Antibody - #DF4025
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| 製品: | RNF138 Antibody |
| カタログ: | DF4025 |
| タンパク質の説明: | Rabbit polyclonal antibody to RNF138 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 28 KD; 28kD(Calculated). |
| ユニプロット: | Q8WVD3 |
| RRID: | AB_2836385 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4025, RRID:AB_2836385.
折りたたみ／展開
E3 ubiquitin protein ligase RNF138; E3 ubiquitin-protein ligase RNF138; hNARF; HSD 4; HSD4; MGC8758; NARF; Nemo like kinase associated RING finger protein; Nemo-like kinase-associated RING finger protein; NLK associated RING finger protein; NLK-associated RING finger protein; RING finger protein 138; ring finger protein 138, E3 ubiquitin protein ligase; RN138_HUMAN; Rnf138; STRIN;
免疫原
- Q8WVD3 RN138_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEDLSAATSYTEDDFYCPVCQEVLKTPVRTTACQHVFCRKCFLTAMRESGAHCPLCRGNVTRRERACPERALDLENIMRKFSGSCRCCAKQIKFYRMRHHYKSCKKYQDEYGVSSIIPNFQISQDSVGNSNRSETSTSDNTETYQENTSSSGHPTFKCPLCQESNFTRQRLLDHCNSNHLFQIVPVTCPICVSLPWGDPSQITRNFVSHLNQRHQFDYGEFVNLQLDEETQYQTAVEESFQVNI
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8WVD3 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| T27 | Phosphorylation | Uniprot | |
| K41 | Acetylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| R80 | Methylation | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| Y108 | Phosphorylation | Uniprot | |
| Y112 | Phosphorylation | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| T142 | Phosphorylation | Uniprot | |
| T144 | Phosphorylation | Uniprot | |
| Y145 | Phosphorylation | Uniprot | |
| T149 | Phosphorylation | Uniprot | |
| K158 | Ubiquitination | Uniprot |
研究背景
E3 ubiquitin-protein ligase involved in DNA damage response by promoting DNA resection and homologous recombination. Recruited to sites of double-strand breaks following DNA damage and specifically promotes double-strand break repair via homologous recombination. Two different, non-exclusive, mechanisms have been proposed. According to a report, regulates the choice of double-strand break repair by favoring homologous recombination over non-homologous end joining (NHEJ): acts by mediating ubiquitination of XRCC5/Ku80, leading to remove the Ku complex from DNA breaks, thereby promoting homologous recombination. According to another report, cooperates with UBE2Ds E2 ubiquitin ligases (UBE2D1, UBE2D2, UBE2D3 or UBE2D4) to promote homologous recombination by mediating ubiquitination of RBBP8/CtIP. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway.
Auto-ubiquitinated.
Chromosome.
Note: Recruited at DNA damage sites (PubMed:26502055). Localizes to sites of double-strand break: localization to double-strand break sites is mediated by the zinc fingers (PubMed:26502055, PubMed:26502057).
Interacts with NLK. Interacts with XRCC5/Ku80. Interacts with RBBP8/CtIP.
The zinc finger domains (C2H2-type and C2HC-type zinc fingers) bind DNA and mediate recruitment to double-strand break sites. They show strong preference for DNA with 5'- or 3'-single-stranded overhangs, while they do not bind blunt-ended double-stranded DNA or poly(ADP-ribose) (PAR) polymers.
参考文献
Application: WB Species: human Sample: human glioma cell lines U87 and U251
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