製品: NDUFB9 Antibody
カタログ: DF4208
タンパク質の説明: Rabbit polyclonal antibody to NDUFB9
アプリケーション: WB IF/ICC
反応性: Human
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 22 KD; 22kD(Calculated).
ユニプロット: Q9Y6M9
RRID: AB_2836416

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(91%), Chicken(91%), Xenopus(82%)
クローナリティ:
Polyclonal
特異性:
NDUFB9 Antibody detects endogenous levels of total NDUFB9.
RRID:
AB_2836416
引用形式: Affinity Biosciences Cat# DF4208, RRID:AB_2836416.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

B22; CI B22; CI-B22; complex I B22 subunit; Complex I-B22; DKFZp566O173; FLJ22885; I B22; LYR motif containing protein 3; LYR motif-containing protein 3; LYRM3; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; NADH ubiquinone oxidoreductase B22 subunit; NADH-ubiquinone oxidoreductase B22 subunit; NDUB9_HUMAN; Ndufb9; UQOR22;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MAFLASGPYLTHQQKVLRLYKRALRHLESWCVQRDKYRYFACLMRARFEEHKNEKDMAKATQLLKEAEEEFWYRQHPQPYIFPDSPGGTSYERYDCYKVPEWCLDDWHPSEKAMYPDYFAKREQWKKLRRESWEREVKQLQEETPPGGPLTEALPPARKEGDLPPLWWYIVTRPRERPM

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Pig
91
Dog
91
Chicken
91
Xenopus
82
Zebrafish
73
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6M9 基板として

Site PTM Type Enzyme
A2 Acetylation
S6 Phosphorylation
Y9 Phosphorylation
K15 Ubiquitination
K55 Acetylation
K59 Ubiquitination
K65 Ubiquitination
Y73 Phosphorylation
Y80 Phosphorylation
S85 Phosphorylation
T89 Phosphorylation
Y91 Phosphorylation
K98 Acetylation
K98 Ubiquitination
K112 Ubiquitination
Y115 Phosphorylation
Y118 Phosphorylation
K121 Acetylation
K121 Ubiquitination
S132 Phosphorylation
K138 Ubiquitination
Y169 Phosphorylation

研究背景

機能:

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

細胞の位置付け:

Mitochondrion inner membrane>Peripheral membrane protein>Matrix side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Mammalian complex I is composed of 45 different subunits.

タンパク質ファミリー:

Belongs to the complex I LYR family.

研究領域

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

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For Research Use Only.
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