製品: EIF2AK1 Antibody
カタログ: DF4051
タンパク質の説明: Rabbit polyclonal antibody to EIF2AK1
アプリケーション: WB IF/ICC
反応性: Human, Mouse
予測: Dog
分子量: 71 KD; 71kD(Calculated).
ユニプロット: Q9BQI3
RRID: AB_2836425

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Dog(86%)
クローナリティ:
Polyclonal
特異性:
EIF2AK1 Antibody detects endogenous levels of total EIF2AK1.
RRID:
AB_2836425
引用形式: Affinity Biosciences Cat# DF4051, RRID:AB_2836425.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

E2AK1_HUMAN; EC 2.7.11.1; Eif2ak1; Eukaryotic translation initiation factor 2 alpha kinase 1; Eukaryotic translation initiation factor 2-alpha kinase 1; HCR; Heme controlled repressor; Heme regulated eukaryotic initiation factor eIF 2 alpha kinase; Heme regulated inhibitor; Heme regulated initiation factor 2 alpha kinase; Heme regulated repressor; Heme sensitive initiation factor 2a kinase; Heme-controlled repressor; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; Hemin sensitive initiation factor 2 alpha kinase; Hemin-sensitive initiation factor 2-alpha kinase; HRI; KIAA1369; PRO1362;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q9BQI3 E2AK1_HUMAN:

Expressed predominantly in erythroid cells. At much lower levels, expressed in hepatocytes (at protein level).

タンパク質配列:
MQGGNSGVRKREEEGDGAGAVAAPPAIDFPAEGPDPEYDESDVPAEIQVLKEPLQQPTFPFAVANQLLLVSLLEHLSHVHEPNPLRSRQVFKLLCQTFIKMGLLSSFTCSDEFSSLRLHHNRAITHLMRSAKERVRQDPCEDISRIQKIRSREVALEAQTSRYLNEFEELAILGKGGYGRVYKVRNKLDGQYYAIKKILIKGATKTVCMKVLREVKVLAGLQHPNIVGYHTAWIEHVHVIQPRADRAAIELPSLEVLSDQEEDREQCGVKNDESSSSSIIFAEPTPEKEKRFGESDTENQNNKSVKYTTNLVIRESGELESTLELQENGLAGLSASSIVEQQLPLRRNSHLEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVDESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACTDILQKNTDWTNRNGKRTPTHTSRVGTCLYASPEQLEGSEYDAKSDMYSLGVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYIQHLTRRNSSQRPSAIQLLQSELFQNSGNVNLTLQMKIIEQEKEIAELKKQLNLLSQDKGVRDDGKDGGVG

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
86
Horse
79
Bovine
79
Sheep
79
Pig
71
Xenopus
43
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BQI3 基板として

Site PTM Type Enzyme
S6 Phosphorylation
S144 Phosphorylation
K175 Ubiquitination
K187 Ubiquitination
K196 Ubiquitination
K201 Ubiquitination
K210 Ubiquitination
S253 Phosphorylation
S258 Phosphorylation
S274 Phosphorylation
S275 Phosphorylation
S276 Phosphorylation
T285 Phosphorylation
K288 Ubiquitination
S295 Phosphorylation
T297 Phosphorylation
K303 Ubiquitination
K472 Ubiquitination
S498 Phosphorylation
K558 Ubiquitination
K602 Ubiquitination
K609 Ubiquitination
K618 Ubiquitination

PTMs - Q9BQI3 酵素として

Substrate Site Source
P05198 (EIF2S1) S49 Uniprot
P05198 (EIF2S1) S52 Uniprot

研究背景

機能:

Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions (By similarity).

PTMs:

Activated by autophosphorylation; phosphorylated predominantly on serine and threonine residues, but also on tyrosine residues. Autophosphorylation at Thr-488 is required for kinase activation. The active autophosphorylated form apparently is largely refractory to cellular heme fluctuations (By similarity).

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed predominantly in erythroid cells. At much lower levels, expressed in hepatocytes (at protein level).

サブユニット構造:

Synthesized in an inactive form that binds to the N-terminal domain of CDC37. Has to be associated with a multiprotein complex containing Hsp90, CDC37 and PPP5C for maturation and activation by autophosphorylation. The phosphatase PPP5C modulates this activation. Forms oligomers. Has been reported as a non-covalently bound homodimer, as well as a hexamer in the absence of hemin. Converted to an inactive disulfide linked homodimer in the presence of hemin (By similarity).

タンパク質ファミリー:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.

研究領域

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

Restrictive clause

 

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