EIF5B Antibody - #DF4055
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4055, RRID:AB_2836429.
折りたたみ／展開
DKFZp434I036; eIF 5B; eIF-5B; Eif5b; Eukaryotic translation initiation factor 5B; FLJ10524; IF2; IF2P_HUMAN; KIAA0741; Translation initiation factor IF 2; Translation initiation factor IF-2; Translation initiation factor IF2;
免疫原
- O60841 IF2P_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGKKQKNKSEDSTKDDIDLDALAAEIEGAGAAKEQEPQKSKGKKKKEKKKQDFDEDDILKELEELSLEAQGIKADRETVAVKPTENNEEEFTSKDKKKKGQKGKKQSFDDNDSEELEDKDSKSKKTAKPKVEMYSGSDDDDDFNKLPKKAKGKAQKSNKKWDGSEEDEDNSKKIKERSRINSSGESGDESDEFLQSRKGQKKNQKNKPGPNIESGNEDDDASFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELETGKKDQSKQKESQRKFEEETVKSKVTVDTGVIPASEEKAETPTAAEDDNEGDKKKKDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKRKKIPQQLESKEVSESMELCAAVEVMEQGVPEKEETPPPVEPEEEEDTEDAGLDDWEAMASDEETEKVEGNKVHIEVKENPEEEEEEEEEEEEDEESEEEEEEEGESEGSEGDEEDEKVSDEKDSGKTLDKKPSKEMSSDSEYDSDDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLRAPIICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEIPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKIKILPQYIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O60841 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S9 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| T13 | Phosphorylation | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| T78 | Phosphorylation | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| Y134 | Phosphorylation | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| T301 | Phosphorylation | Uniprot | |
| T303 | Phosphorylation | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| S411 | Phosphorylation | Uniprot | |
| K424 | Ubiquitination | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| S438 | Phosphorylation | Uniprot | |
| Y446 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| T486 | Phosphorylation | Uniprot | |
| T498 | Phosphorylation | Uniprot | |
| S511 | Phosphorylation | Uniprot | |
| T515 | Phosphorylation | Uniprot | |
| S547 | Phosphorylation | Uniprot | |
| S557 | Phosphorylation | Uniprot | |
| S560 | Phosphorylation | Uniprot | |
| S584 | Phosphorylation | Uniprot | |
| S588 | Phosphorylation | Uniprot | |
| S589 | Phosphorylation | Uniprot | |
| S591 | Phosphorylation | Uniprot | |
| Y593 | Phosphorylation | Uniprot | |
| S595 | Phosphorylation | Uniprot | |
| K745 | Ubiquitination | Uniprot | |
| Y762 | Phosphorylation | Uniprot | |
| K766 | Ubiquitination | Uniprot | |
| S767 | Phosphorylation | Uniprot | |
| S770 | Phosphorylation | Uniprot | |
| T775 | Phosphorylation | Uniprot | |
| K784 | Acetylation | Uniprot | |
| K883 | Ubiquitination | Uniprot | |
| K923 | Acetylation | Uniprot | |
| K923 | Ubiquitination | Uniprot | |
| K932 | Ubiquitination | Uniprot | |
| K936 | Ubiquitination | Uniprot | |
| K968 | Acetylation | Uniprot | |
| K968 | Ubiquitination | Uniprot | |
| T1000 | Phosphorylation | Uniprot | |
| S1001 | Phosphorylation | Uniprot | |
| C1092 | S-Nitrosylation | Uniprot | |
| K1093 | Ubiquitination | Uniprot | |
| K1095 | Ubiquitination | Uniprot | |
| Y1100 | Phosphorylation | Uniprot | |
| S1104 | Phosphorylation | Uniprot | |
| K1153 | Ubiquitination | Uniprot | |
| K1160 | Acetylation | Uniprot | |
| S1168 | Phosphorylation | Uniprot | |
| K1170 | Acetylation | Uniprot | |
| K1170 | Ubiquitination | Uniprot | |
| S1184 | Phosphorylation | Uniprot | |
| K1185 | Ubiquitination | Uniprot | |
| K1214 | Acetylation | Uniprot |
研究背景
Plays a role in translation initiation. Translational GTPase that catalyzes the joining of the 40S and 60S subunits to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. GTP binding and hydrolysis induces conformational changes in the enzyme that renders it active for productive interactions with the ribosome. The release of the enzyme after formation of the initiation complex is a prerequisite to form elongation-competent ribosomes.
(Microbial infection) Cleaved and inactivated by the protease 3C of poliovirus, Coxsackievirus B3 and Human rhinovirus 14, allowing the virus to shutoff the host cell translation.
Cytoplasm.
Interacts with ANXA5 in a calcium and phospholipid-dependent manner.
研究領域
· Genetic Information Processing > Translation > RNA transport.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.