製品: ALDOB Antibody
カタログ: DF4064
タンパク質の説明: Rabbit polyclonal antibody to ALDOB
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Rabbit, Dog
分子量: 39 KD; 39kD(Calculated).
ユニプロット: P05062
RRID: AB_2836438

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(83%), Horse(83%), Rabbit(100%), Dog(92%)
クローナリティ:
Polyclonal
特異性:
ALDOB Antibody detects endogenous levels of total ALDOB.
RRID:
AB_2836438
引用形式: Affinity Biosciences Cat# DF4064, RRID:AB_2836438.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

ALDB; ALDO B; ALDO2; ALDOB; ALDOB_HUMAN; Aldolase 2; Aldolase B; Aldolase B fructose bisphosphate; Aldolase2; AldolaseB; EC 4.1.2.13; Fructose bisphosphate aldolase B; Fructose-bisphosphate aldolase B; Liver type aldolase; Liver-type aldolase; MS1077;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MAHRFPALTQEQKKELSEIAQSIVANGKGILAADESVGTMGNRLQRIKVENTEENRRQFREILFSVDSSINQSIGGVILFHETLYQKDSQGKLFRNILKEKGIVVGIKLDQGGAPLAGTNKETTIQGLDGLSERCAQYKKDGVDFGKWRAVLRIADQCPSSLAIQENANALARYASICQQNGLVPIVEPEVIPDGDHDLEHCQYVTEKVLAAVYKALNDHHVYLEGTLLKPNMVTAGHACTKKYTPEQVAMATVTALHRTVPAAVPGICFLSGGMSEEDATLNLNAINLCPLPKPWKLSFSYGRALQASALAAWGGKAANKEATQEAFMKRAMANCQAAKGQYVHTGSSGAASTQSLFTACYTY

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
92
Horse
83
Bovine
83
Sheep
75
Xenopus
67
Pig
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05062 基板として

Site PTM Type Enzyme
K13 Acetylation
S36 Phosphorylation
T39 Phosphorylation
S89 Phosphorylation
T119 Phosphorylation
T123 Phosphorylation
T124 Phosphorylation
S132 Phosphorylation
K139 Acetylation
K147 Acetylation
S161 Phosphorylation
Y204 Phosphorylation
T206 Phosphorylation
K208 Acetylation
K208 Ubiquitination
Y214 Phosphorylation
K215 Methylation
Y223 Phosphorylation
T241 Phosphorylation
K242 Acetylation
K243 Acetylation
C269 S-Nitrosylation
S272 Phosphorylation
S276 Phosphorylation
Y302 Phosphorylation
S309 Phosphorylation
K330 Acetylation
Y362 Phosphorylation
T363 Phosphorylation

研究背景

細胞の位置付け:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriolar satellite.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homotetramer. Interacts with BBS1, BBS2, BBS4 and BBS7.

タンパク質ファミリー:

Belongs to the class I fructose-bisphosphate aldolase family.

研究領域

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Pentose phosphate pathway.

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

参考文献

1). SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis. FREE RADICAL BIOLOGY AND MEDICINE, 2019 (PubMed: 30703481) [IF=7.4]

Application: WB    Species: human    Sample: kidney

Fig. S2.| Verification of the differential succinylation of proteins found by MS/MS assay. These proteins were immunoprecipitated by corresponding antibodies and detected with pan anti-succinyllysine antibody. Adj, adjacent normal tissues. T, ccRCC tissues.

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