GLRB Antibody - #DF4088
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4088, RRID:AB_2836462.
折りたたみ／展開
Glycine receptor 58 kDa subunit; Glycine receptor beta; Glycine receptor subunit beta; Glycine receptor, beta subunit; OTTHUMP00000220896; OTTHUMP00000220897;
免疫原
- P48167 GLRB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKFLLTTAFLILISLWVEEAYSKEKSSKKGKGKKKQYLCPSQQSAEDLARVPANSTSNILNRLLVSYDPRIRPNFKGIPVDVVVNIFINSFGSIQETTMDYRVNIFLRQKWNDPRLKLPSDFRGSDALTVDPTMYKCLWKPDLFFANEKSANFHDVTQENILLFIFRDGDVLVSMRLSITLSCPLDLTLFPMDTQRCKMQLESFGYTTDDLRFIWQSGDPVQLEKIALPQFDIKKEDIEYGNCTKYYKGTGYYTCVEVIFTLRRQVGFYMMGVYAPTLLIVVLSWLSFWINPDASAARVPLGIFSVLSLASECTTLAAELPKVSYVKALDVWLIACLLFGFASLVEYAVVQVMLNNPKRVEAEKARIAKAEQADGKGGNVAKKNTVNGTGTPVHISTLQVGETRCKKVCTSKSDLRSNDFSIVGSLPRDFELSNYDCYGKPIEVNNGLGKSQAKNNKKPPPAKPVIPTAAKRIDLYARALFPFCFLFFNVIYWSIYL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P48167 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K28 | Acetylation | Uniprot | |
| K31 | Acetylation | Uniprot | |
| K34 | Acetylation | Uniprot | |
| K35 | Acetylation | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| T129 | Phosphorylation | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| Y206 | Phosphorylation | Uniprot | |
| Y274 | Phosphorylation | Uniprot | |
| T277 | Phosphorylation | Uniprot | |
| K369 | Ubiquitination | Uniprot | |
| S411 | Phosphorylation | Uniprot | |
| Y435 | Phosphorylation | P12931 (SRC) | Uniprot |
| K440 | Ubiquitination | Uniprot |
研究背景
Glycine receptors are ligand-gated chloride channels. GLRB does not form ligand-gated ion channels by itself, but is part of heteromeric ligand-gated chloride channels. Channel opening is triggered by extracellular glycine. Heteropentameric channels composed of GLRB and GLRA1 are activated by lower glycine levels than homopentameric GLRA1. Plays an important role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents.
Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Cell junction>Synapse. Cell projection>Dendrite. Cell membrane>Multi-pass membrane protein. Cytoplasm.
Note: Retained in the cytoplasm upon heterologous expression by itself. Coexpression with GPHN promotes expression at the cell membrane (PubMed:12684523). Coexpression with GLRA1, GLRA2 or GLRA3 promotes expression at the cell membrane.
Heteropentamer composed of GLRB and GLRA1. Heteropentamer composed of GLRB and GLRA2. Heteropentamer composed of GLRB and GLRA3. Heteropentamer composed of two GLRA1 and three GLRB subunits. Heteropentamer composed of three GLRA1 and two GLRB subunits. Interacts with GPHN.
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRB sub-subfamily.
研究領域
· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.
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