GAS1 Antibody - #DF4098

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製品: GAS1 Antibody
カタログ: DF4098
タンパク質の説明: Rabbit polyclonal antibody to GAS1
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
分子量: 36 KD; 36kD(Calculated).
ユニプロット: P54826
RRID: AB_2836472

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
GAS1 Antibody detects endogenous levels of total GAS1.
RRID:
AB_2836472
引用形式: Affinity Biosciences Cat# DF4098, RRID:AB_2836472.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

GAS-1; Gas1; GAS1_HUMAN; Growth arrest-specific protein 1; Growth-arrest-specific protein 1 precursor;

免疫原

免疫原:

A synthesized peptide derived from human GAS1, corresponding to a region within the internal amino acids.

Uniprot:

P54826(GAS1_HUMAN) >>Visit HPA database.

遺伝子(ID):
GAS1(2619)
タンパク質配列:
MVAALLGGGGEARGGTVPGAWLCLMALLQLLGSAPRGSGLAHGRRLICWQALLQCQGEPECSYAYNQYAEACAPVLAQHGGGDAPGAAAAAFPASAASFSSRWRCPSHCISALIQLNHTRRGPALEDCDCAQDENCKSTKRAIEPCLPRTSGGGAGGPGAGGVMGCTEARRRCDRDSRCNLALSRYLTYCGKVFNGLRCTDECRTVIEDMLAMPKAALLNDCVCDGLERPICESVKENMARLCFGAELGNGPGSSGSDGGLDDYYDEDYDDEQRTGGAGGEQPLDDDDGVPHPPRPGSGAAASGGRGDLPYGPGRRSSGGGGRLAPRGAWTPLASILLLLLGPLF

研究背景

機能:

Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells.

細胞の位置付け:

Cell membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

研究領域

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

参考文献

1). Multi-omics analysis unveiled fibroblast- mediated pathogenesis in male genital lichen sclerosus. Cell and Bioscience, 2025 [IF=6.2]

Application: WB    Species: human    Sample:

Fig. 9 Employing the multi-omics approach to identify additional susceptible genes in LS disease. A.The flowchart of the multi-omics analysis. B.The integration of the SMR data with the scRNA-seq data ultimately yielded two susceptible genes in LS disease. C.The expression profiles of the two filtered-out genes among all the cell types. D.The protein expression level of the two filtered-out genes among the three groups. E.The quantitative analysis of the protein expression level in Fig. 9D. The data were presented as the mean ± SEM (n = 3). F.The protein expression level of the GAS1, COL1A1, and COL6A1 genes in shRNA plasmid-transfected HFF-1. G.The expression profiles of the TGFB1 gene between GAS1+ and GAS1- fibroblasts. H.The protein expression level of the GAS1, COL1A1, and COL6A1 genes in urea-stimulated HFF-1 at different concentrations. cis-eQTL: cis-expression quantitative trait locus; GWAS: genome-wide association study; SMR: summary-based Mendelian randomization; SEM: standard error of the mean; shRNA: short-hairpin RNA; HFF-1: human foreskin fibroblasts
2). GAS1 affects proliferation, apoptosis, and steroid hormone levels by regulating mitochondrial functions and the PI3K/AKT pathway in bovine granulosa cells. THERIOGENOLOGY, 2025 [IF=2.5]

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