KCNN4 Antibody - #DF4132

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製品: KCNN4 Antibody
カタログ: DF4132
タンパク質の説明: Rabbit polyclonal antibody to KCNN4
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Horse, Sheep, Rabbit, Dog
分子量: 48 KD; 48kD(Calculated).
ユニプロット: O15554
RRID: AB_2836497

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Horse(91%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
KCNN4 Antibody detects endogenous levels of total KCNN4.
RRID:
AB_2836497
引用形式: Affinity Biosciences Cat# DF4132, RRID:AB_2836497.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

DHS2; hIKCa1; hKCa4; hSK4; IK; IK1; IKCa1; Intermediate conductance calcium activated potassium channel protein 4; Intermediate conductance calcium-activated potassium channel protein 4; KCa3.1; KCa4; KCNN 4; Kcnn4; KCNN4_HUMAN; Potassium calcium activated channel subfamily N member 4; Potassium intermediate/small conductance calcium activated channel, subfamily N, member 4; Putative Gardos channel; SK4; SKCa 4; SKCa4;

免疫原

免疫原:
Uniprot:

O15554(KCNN4_HUMAN) >>Visit HPA database.

遺伝子(ID):
KCNN4(3783)
発現特異性:
O15554 KCNN4_HUMAN:

Widely expressed in non-excitable tissues.

タンパク質配列:
MGGDLVLGLGALRRRKRLLEQEKSLAGWALVLAGTGIGLMVLHAEMLWFGGCSWALYLFLVKCTISISTFLLLCLIVAFHAKEVQLFMTDNGLRDWRVALTGRQAAQIVLELVVCGLHPAPVRGPPCVQDLGAPLTSPQPWPGFLGQGEALLSLAMLLRLYLVPRAVLLRSGVLLNASYRSIGALNQVRFRHWFVAKLYMNTHPGRLLLGLTLGLWLTTAWVLSVAERQAVNATGHLSDTLWLIPITFLTIGYGDVVPGTMWGKIVCLCTGVMGVCCTALLVAVVARKLEFNKAEKHVHNFMMDIQYTKEMKESAARVLQEAWMFYKHTRRKESHAARRHQRKLLAAINAFRQVRLKHRKLREQVNSMVDISKMHMILYDLQQNLSSSHRALEKQIDTLAGKLDALTELLSTALGPRQLPEPSQQSK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Rabbit
100
Horse
91
Zebrafish
67
Bovine
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15554 基板として

Site PTM Type Enzyme
S178 Phosphorylation
Y199 Phosphorylation
S314 Phosphorylation
T329 Phosphorylation
S334 Phosphorylation P17612 (PRKACA)
H358 Phosphorylation P22392 (NME2)
S367 Phosphorylation
K394 Ubiquitination
T398 Phosphorylation

研究背景

機能:

Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. Plays a role in the late stages of EGF-induced macropinocytosis.

PTMs:

Phosphorylation at His-358 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed in non-excitable tissues.

サブユニット構造:

Heterotetramer of potassium channel proteins (Probable). Interacts with MTMR6.

タンパク質ファミリー:

Belongs to the potassium channel KCNN family. KCa3.1/KCNN4 subfamily.

研究領域

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Protein digestion and absorption.

参考文献

1). A prospective diagnostic and prognostic biomarker for hepatocellular carcinoma that functions in glucose metabolism regulation: Solute carrier family 37 member 3. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2023 (PubMed: 36773462) [IF=6.2]
2). KCNN4 is a potential prognostic marker and critical factor affecting the immune status of the tumor microenvironment in kidney renal clear cell carcinoma. Translational Andrology and Urology, 2021 (PubMed: 34295732) [IF=2.0]

Application: WB    Species: Human    Sample: kidney renal clear cell

Figure 13 Expression of KCNN4 increased in KIRC samples. (A) Expression of KCNN4 in KIRC samples and normal adjacent non-tumor controls were assessed by qPCR (*P<0.05). (B,C) Representative blots and quantification results of western blot showed that expression of the KCNN4 protein in KIRC was significantly higher than controls (*P<0.05). KIRC, kidney renal clear cell carcinoma.

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