MED8 Antibody - #DF4154
| 製品: | MED8 Antibody |
| カタログ: | DF4154 |
| タンパク質の説明: | Rabbit polyclonal antibody to MED8 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 29 KD; 29kD(Calculated). |
| ユニプロット: | Q96G25 |
| RRID: | AB_2836519 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4154, RRID:AB_2836519.
折りたたみ／展開
Activator recruited cofactor 32 kDa component; Activator-recruited cofactor 32 kDa component; ARC32; med8; MED8_HUMAN; Mediator complex subunit 8; Mediator of RNA polymerase II transcription subunit 8; Mediator of RNA polymerase II transcription subunit 8 homolog;
免疫原
- Q96G25 MED8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQREEKQLEASLDALLSQVADLKNSLGSFICKLENEYGRLTWPSVLDSFALLSGQLNTLNKVLKHEKTPLFRNQVIIPLVLSPDRDEDLMRQTEGRVPVFSHEVVPDHLRTKPDPEVEEQEKQLTTDAARIGADAAQKQIQSLNKMCSNLLEKISKEERESESGGLRPNKQTFNPTDTNALVAAVAFGKGLSNWRPSGSSGPGQAGQPGAGTILAGTSGLQQVQMAGAPSQQQPMLSGVQMAQAGQPGKMPSGIKTNIKSASMHPYQR
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96G25 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K6 | Ubiquitination | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| K112 | Sumoylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K145 | Ubiquitination | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| K255 | Acetylation | Uniprot | |
| K259 | Acetylation | Uniprot | |
| K259 | Methylation | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| Y266 | Phosphorylation | Uniprot |
研究背景
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.
Nucleus.
Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. May be part of a multisubunit E3 ubiquitin-protein ligase complex with the elongin BC complex (ELOB and ELOC), CUL2 and RBX1.
The elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
Belongs to the Mediator complex subunit 8 family.
研究領域
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
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