MBTPS1 Antibody - #DF4159
(1)
(5)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4159, RRID:AB_2836524.
折りたたみ／展開
kexin isozyme 1; KIAA0091; MBTPS1; membrane bound transcription factor peptidase, site 1; MGC138711; MGC138712; PCSK8; S1P; S1P endopeptidase; Site 1 protease; SKI 1; SKI1; Subtilisin isozyme 1; Subtilisin/kexin isozyme 1;
免疫原
- Q14703 MBTP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKLVNIWLLLLVVLLCGKKHLGDRLEKKSFEKAPCPGCSHLTLKVEFSSTVVEYEYIVAFNGYFTAKARNSFISSALKSSEVDNWRIIPRNNPSSDYPSDFEVIQIKEKQKAGLLTLEDHPNIKRVTPQRKVFRSLKYAESDPTVPCNETRWSQKWQSSRPLRRASLSLGSGFWHATGRHSSRRLLRAIPRQVAQTLQADVLWQMGYTGANVRVAVFDTGLSEKHPHFKNVKERTNWTNERTLDDGLGHGTFVAGVIASMRECQGFAPDAELHIFRVFTNNQVSYTSWFLDAFNYAILKKIDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVSAIGNDGPLYGTLNNPADQMDVIGVGGIDFEDNIARFSSRGMTTWELPGGYGRMKPDIVTYGAGVRGSGVKGGCRALSGTSVASPVVAGAVTLLVSTVQKRELVNPASMKQALIASARRLPGVNMFEQGHGKLDLLRAYQILNSYKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTVVNVTILNGMGVTGRIVDKPDWQPYLPQNGDNIEVAFSYSSVLWPWSGYLAISISVTKKAASWEGIAQGHVMITVASPAETESKNGAEQTSTVKLPIKVKIIPTPPRSKRVLWDQYHNLRYPPGYFPRDNLRMKNDPLDWNGDHIHTNFRDMYQHLRSMGYFVEVLGAPFTCFDASQYGTLLMVDSEEEYFPEEIAKLRRDVDNGLSLVIFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANIPALNELLSVWNMGFSDGLYEGEFTLANHDMYYASGCSIAKFPEDGVVITQTFKDQGLEVLKQETAVVENVPILGLYQIPAEGGGRIVLYGDSNCLDDSHRQKDCFWLLDALLQYTSYGVTPPSLSHSGNRQRPPSGAGSVTPERMEGNHLHRYSKVLEAHLGDPKPRPLPACPRLSWAKPQPLNETAPSNLWKHQKLLSIDLDKVVLPNFRSNRPQVRPLSPGESGAWDIPGGIMPGRYNQEVGQTIPVFAFLGAMVVLAFFVVQINKAKSRPKRRKPRVKRPQLMQQVHPPKTPSV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14703 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K78 | Ubiquitination | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| S166 | O-Glycosylation | Uniprot | |
| S168 | O-Glycosylation | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| T177 | O-Glycosylation | Uniprot | |
| T177 | Phosphorylation | Uniprot | |
| R213 | Methylation | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| K229 | Ubiquitination | Uniprot | |
| T235 | Phosphorylation | Uniprot | |
| K388 | Ubiquitination | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| S429 | Phosphorylation | Uniprot | |
| K443 | Ubiquitination | Uniprot | |
| T616 | Phosphorylation | Uniprot | |
| K646 | Ubiquitination | Uniprot | |
| T896 | O-Glycosylation | Uniprot | |
| K934 | Ubiquitination | Uniprot | |
| K951 | Ubiquitination | Uniprot | |
| K1036 | Acetylation | Uniprot | |
| K1048 | Acetylation | Uniprot | |
| T1049 | Phosphorylation | Uniprot | |
| S1051 | Phosphorylation | Uniprot |
研究背景
Serine protease that catalyzes the first step in the proteolytic activation of the sterol regulatory element-binding proteins (SREBPs). Other known substrates are BDNF, GNPTAB and ATF6. Cleaves after hydrophobic or small residues, provided that Arg or Lys is in position P4. Cleaves known substrates after Arg-Ser-Val-Leu (SERBP-2), Arg-His-Leu-Leu (ATF6), Arg-Gly-Leu-Thr (BDNF) and its own propeptide after Arg-Arg-Leu-Leu. Mediates the protein cleavage of GNPTAB into subunit alpha and beta, thereby participating in biogenesis of lysosomes. Involved in the regulation of M6P-dependent Golgi-to-lysosome trafficking of lysosomal enzymes. It is required for the activation of CREB3L2/BBF2H7, a transcriptional activator of MIA3/TANGO and other genes controlling mega vesicle formation. Therefore, it plays a key role in the regulation of mega vesicle-mediated collagen trafficking.
The 148 kDa zymogen is processed progressively into two membrane-bound 120 and 106 kDa forms in the endoplasmic reticulum, and late into a secreted 98 kDa form. The propeptide is autocatalytically removed through an intramolecular cleavage after Leu-186. Further cleavage generates 14, 10, and 8 kDa intermediates.
Endoplasmic reticulum membrane>Single-pass type I membrane protein. Golgi apparatus membrane>Single-pass type I membrane protein.
Note: May sort to other organelles, including lysosomal and/or endosomal compartments.
Widely expressed.
Belongs to the peptidase S8 family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
参考文献
Application: WB Species: Rat Sample: liver
Application: WB Species: Rat Sample: liver
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