MT-ND1 Antibody - #DF4214

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製品: MT-ND1 Antibody
カタログ: DF4214
タンパク質の説明: Rabbit polyclonal antibody to MT-ND1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 36 KD; 36kD(Calculated).
ユニプロット: P03886
RRID: AB_2836565

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(90%), Zebrafish(90%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(90%), Dog(90%), Chicken(90%), Xenopus(90%)
クローナリティ:
Polyclonal
特異性:
MT-ND1 Antibody detects endogenous levels of total MT-ND1.
RRID:
AB_2836565
引用形式: Affinity Biosciences Cat# DF4214, RRID:AB_2836565.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Complex I, subunit ND1; Mitochondrially encoded NADH dehydrogenase 1; MT-ND1; MTND1; NAD1; NADH dehydrogenase subunit 1 (complex I); NADH dehydrogenase subunit 1; NADH-ubiquinone oxidoreductase chain 1; NADH-ubiquinone oxidoreductase, subunit ND1; NADH1; ND1; NU1M_HUMAN;

免疫原

免疫原:
Uniprot:

P03886(NU1M_HUMAN) >>Visit HPA database.

遺伝子(ID):
MT-ND1(4535)
タンパク質配列:
MPMANLLLLIVPILIAMAFLMLTERKILGYMQLRKGPNVVGPYGLLQPFADAMKLFTKEPLKPATSTITLYITAPTLALTIALLLWTPLPMPNPLVNLNLGLLFILATSSLAVYSILWSGWASNSNYALIGALRAVAQTISYEVTLAIILLSTLLMSGSFNLSTLITTQEHLWLLLPSWPLAMMWFISTLAETNRTPFDLAEGESELVSGFNIEYAAGPFALFFMAEYTNIIMMNTLTTTIFLGTTYDALSPELYTTYFVTKTLLLTSLFLWIRTAYPRFRYDQLMHLLWKNFLPLTLALLMWYVSMPITISSIPPQT

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Horse
90
Bovine
90
Sheep
90
Dog
90
Xenopus
90
Zebrafish
90
Chicken
90
Rabbit
90
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).

細胞の位置付け:

Mitochondrion inner membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the complex I subunit 1 family.

研究領域

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

参考文献

1). Increased Mitochondrial Fragmentation Mediated by Dynamin-Related Protein 1 Contributes to Hexavalent Chromium-Induced Mitochondrial Respiratory Chain Complex I-Dependent Cytotoxicity. Toxics, 2020 (PubMed: 32751088) [IF=4.6]

Application: WB    Species: human    Sample: L02 hepatocytes

Figure 3. |Cr(VI) targeted MRCC I to induce cytotoxicity. (A) L02 hepatocytes were treated with different concentrations of Cr(VI) (0, 8, 16 µM) for 24 h. The activities of MRCC I-V were determined spectrophotometrically using the commercial kits. (B) The protein expression of ND1 was determined using Western blotting.
2). Cr (VI)-induced overactive mitophagy contributes to mitochondrial loss and cytotoxicity in L02 hepatocytes. Biochemical Journal, 2020 (PubMed: 32597464) [IF=4.1]

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