製品: OAT Antibody
カタログ: DF4270
タンパク質の説明: Rabbit polyclonal antibody to OAT
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 48 KD; 49kD(Calculated).
ユニプロット: P04181
RRID: AB_2836621

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(82%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(80%), Chicken(91%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
OAT Antibody detects endogenous levels of total OAT.
RRID:
AB_2836621
引用形式: Affinity Biosciences Cat# DF4270, RRID:AB_2836621.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

GACR; Gyrate atrophy; HOGA; OAT; OATASE; OKT; Ornithine aminotransferase (gyrate atrophy); Ornithine aminotransferase mitochondrial; ornithine aminotransferase precursor; Ornithine aminotransferase, mitochondrial precursor; Ornithine delta aminotransferase; Ornithine oxo acid aminotransferase;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MFSKLAHLQRFAVLSRGVHSSVASATSVATKKTVQGPPTSDDIFEREYKYGAHNYHPLPVALERGKGIYLWDVEGRKYFDFLSSYSAVNQGHCHPKIVNALKSQVDKLTLTSRAFYNNVLGEYEEYITKLFNYHKVLPMNTGVEAGETACKLARKWGYTVKGIQKYKAKIVFAAGNFWGRTLSAISSSTDPTSYDGFGPFMPGFDIIPYNDLPALERALQDPNVAAFMVEPIQGEAGVVVPDPGYLMGVRELCTRHQVLFIADEIQTGLARTGRWLAVDYENVRPDIVLLGKALSGGLYPVSAVLCDDDIMLTIKPGEHGSTYGGNPLGCRVAIAALEVLEEENLAENADKLGIILRNELMKLPSDVVTAVRGKGLLNAIVIKETKDWDAWKVCLRLRDNGLLAKPTHGDIIRFAPPLVIKEDELRESIEIINKTILSF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Chicken
91
Pig
82
Zebrafish
82
Rabbit
80
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P04181 基板として

Site PTM Type Enzyme
T30 Phosphorylation
K32 Ubiquitination
T39 Phosphorylation
K49 Ubiquitination
Y55 Phosphorylation
K66 Ubiquitination
Y69 Phosphorylation
Y85 Phosphorylation
K96 Acetylation
K102 Ubiquitination
Y123 Phosphorylation
Y126 Phosphorylation
Y133 Phosphorylation
K151 Ubiquitination
T272 Phosphorylation
Y280 Phosphorylation
C306 S-Nitrosylation
C330 S-Nitrosylation
K362 Ubiquitination
S365 Phosphorylation
K374 Ubiquitination
K386 Ubiquitination
K392 Ubiquitination
K405 Ubiquitination
R426 Methylation

研究背景

細胞の位置付け:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homohexamer.

タンパク質ファミリー:

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.

研究領域

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

参考文献

1). Diterpenoid alkaloids isolated from Delphinium trichophorum alleviate pulmonary fibrosis via the TGF-β/Smad pathway in 3T6 and HFL-1 cells. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2022 (PubMed: 36068772) [IF=7.5]

2). Inactivation of PDH can Reduce Anaplastic Thyroid Cancer Cells' Sensitivity to Artemisinin. Anti-Cancer Agents in Medicinal Chemistry, 2022 (PubMed: 34515013) [IF=2.8]

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