PNN Antibody - #DF4296

(1)  
製品: PNN Antibody
カタログ: DF4296
タンパク質の説明: Rabbit polyclonal antibody to PNN
アプリケーション: WB IF/ICC
反応性: Human, Mouse
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 85 KD; 82kD(Calculated).
ユニプロット: Q9H307
RRID: AB_2836647

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
PNN Antibody detects endogenous levels of total PNN.
RRID:
AB_2836647
引用形式: Affinity Biosciences Cat# DF4296, RRID:AB_2836647.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

140 kDa nuclear and cell adhesion-related phosphoprotein; Desmosome-associated protein; Domain-rich serine protein; DRS; DRS protein; DRSP; Melanoma metastasis clone A protein; MEMA; Nuclear protein SDK3; Pinin; PININ_HUMAN; PNN; SR like protein; SR-like protein;

免疫原

免疫原:
Uniprot:

Q9H307(PININ_HUMAN) >>Visit HPA database.

遺伝子(ID):
PNN(5411)
発現特異性:
Q9H307 PININ_HUMAN:

Expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, epidermis, esophagus, brain and smooth and skeletal muscle. Expressed strongly in melanoma metastasis lesions and advanced primary tumors.

タンパク質配列:
MAVAVRTLQEQLEKAKESLKNVDENIRKLTGRDPNDVRPIQARLLALSGPGGGRGRGSLLLRRGFSDSGGGPPAKQRDLEGAVSRLGGERRTRRESRQESDPEDDDVKKPALQSSVVATSKERTRRDLIQDQNMDEKGKQRNRRIFGLLMGTLQKFKQESTVATERQKRRQEIEQKLEVQAEEERKQVENERRELFEERRAKQTELRLLEQKVELAQLQEEWNEHNAKIIKYIRTKTKPHLFYIPGRMCPATQKLIEESQRKMNALFEGRRIEFAEQINKMEARPRRQSMKEKEHQVVRNEEQKAEQEEGKVAQREEELEETGNQHNDVEIEEAGEEEEKEIAIVHSDAEKEQEEEEQKQEMEVKMEEETEVRESEKQQDSQPEEVMDVLEMVENVKHVIADQEVMETNRVESVEPSENEASKELEPEMEFEIEPDKECKTLSPGKENVSALDMEKESEEKEEKESEPQPEPVAQPQPQSQPQLQLQSQSQPVLQSQPPSQPEDLSLAVLQPTPQVTQEQGHLLPERKDFPVESVKLTEVPVEPVLTVHPESKSKTKTRSRSRGRARNKTSKSRSRSSSSSSSSSSSTSSSSGSSSSSGSSSSRSSSSSSSSTSGSSSRDSSSSTSSSSESRSRSRGRGHNRDRKHRRSVDRKRRDTSGLERSHKSSKGGSSRDTKGSKDKNSRSDRKRSISESSRSGKRSSRSERDRKSDRKDKRR

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
89
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H307 基板として

Site PTM Type Enzyme
A2 Acetylation
T30 Phosphorylation
S48 Phosphorylation
R54 Methylation
R56 Methylation
S58 Phosphorylation
R62 Methylation
R63 Methylation
S66 Phosphorylation
S68 Phosphorylation
S84 Phosphorylation
T92 Phosphorylation
S96 Phosphorylation
S100 Phosphorylation
K109 Sumoylation
K109 Ubiquitination
S114 Phosphorylation
S115 Phosphorylation
S120 Phosphorylation
K121 Ubiquitination
T124 Phosphorylation
K137 Ubiquitination
T152 Phosphorylation
K157 Methylation
K157 Sumoylation
K157 Ubiquitination
S160 Phosphorylation
T164 Phosphorylation
K212 Ubiquitination
K228 Ubiquitination
K238 Acetylation
K238 Ubiquitination
Y243 Phosphorylation
K262 Ubiquitination
K280 Ubiquitination
S289 Phosphorylation
K304 Sumoylation
S347 Phosphorylation
S375 Phosphorylation
S381 Phosphorylation
T408 Phosphorylation
S413 Phosphorylation
S417 Phosphorylation
S422 Phosphorylation
S443 Phosphorylation
S450 Phosphorylation
S458 Phosphorylation
K528 Ubiquitination
K536 Ubiquitination
T547 Phosphorylation
S552 Phosphorylation
K553 Sumoylation
T558 Phosphorylation
S560 Phosphorylation
S575 Phosphorylation
S578 Phosphorylation
S579 Phosphorylation
S580 Phosphorylation
S581 Phosphorylation
S582 Phosphorylation
S583 Phosphorylation
S587 Phosphorylation
S589 Phosphorylation
S590 Phosphorylation
S591 Phosphorylation
S594 Phosphorylation
S597 Phosphorylation
S600 Phosphorylation
S601 Phosphorylation
S602 Phosphorylation
S603 Phosphorylation
R604 Methylation
S605 Phosphorylation
S611 Phosphorylation
S618 Phosphorylation
S621 Phosphorylation
S623 Phosphorylation
S624 Phosphorylation
S629 Phosphorylation
S633 Phosphorylation
S649 Phosphorylation
T657 Phosphorylation
S658 Phosphorylation
S663 Phosphorylation
S666 Phosphorylation
S667 Phosphorylation
K668 Acetylation
S671 Phosphorylation
S672 Phosphorylation
S690 Phosphorylation
S692 Phosphorylation
S694 Phosphorylation
S695 Phosphorylation
S697 Phosphorylation
S702 Phosphorylation
S704 Phosphorylation

研究背景

機能:

Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Component of the PSAP complex which binds RNA in a sequence-independent manner and is proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma.

細胞の位置付け:

Nucleus speckle. Cell junction>Desmosome.
Note: Cell-cell contact area, predominantly desmosome of intercellular adherens junction. Not a nucleocytoplasmic shuttling protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, epidermis, esophagus, brain and smooth and skeletal muscle. Expressed strongly in melanoma metastasis lesions and advanced primary tumors.

サブユニット構造:

Found in a mRNA splicing-dependent exon junction complex (EJC). Found in a complex with SR proteins. Found in a mRNP complex with RNPS1. Component of the PSAP complex consisting of RNPS1, SAP18 and PNN. Interacts with PNISR, CTBP1, CTBP2, KRT8, KRT18, KRT19, PS1D/PNO40, PPIG, RNPS1, SFRS4 and SRRM2. Identified in the spliceosome C complex.

タンパク質ファミリー:

Belongs to the pinin family.

研究領域

· Genetic Information Processing > Translation > RNA transport.

· Genetic Information Processing > Translation > mRNA surveillance pathway.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.