PKP2 Antibody - #DF4297

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製品: PKP2 Antibody
カタログ: DF4297
タンパク質の説明: Rabbit polyclonal antibody to PKP2
アプリケーション: WB IF/ICC
反応性: Human, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 97 KD; 97kD(Calculated).
ユニプロット: Q99959
RRID: AB_2836648

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MS Report
HPLC Report
MSDS
説明書
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プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
予測:
Pig(92%), Bovine(92%), Horse(92%), Sheep(83%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
PKP2 Antibody detects endogenous levels of total PKP2.
RRID:
AB_2836648
引用形式: Affinity Biosciences Cat# DF4297, RRID:AB_2836648.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ARVD 9; ARVD9; PKP 2; PKP2; PKP2_HUMAN; Plakophilin-2; Plakophilin2;

免疫原

免疫原:
Uniprot:

Q99959(PKP2_HUMAN) >>Visit HPA database.

遺伝子(ID):
PKP2(5318)
発現特異性:
Q99959 PKP2_HUMAN:

Detected in heart right ventricle (at protein level). Widely expressed. Found at desmosomal plaques in simple and stratified epithelia and in non-epithelial tissues such as myocardium and lymph node follicles. In most stratified epithelia found in the desmosomes of the basal cell layer and seems to be absent from suprabasal strata.

タンパク質配列:
MAAPGAPAEYGYIRTVLGQQILGQLDSSSLALPSEAKLKLAGSSGRGGQTVKSLRIQEQVQQTLARKGRSSVGNGNLHRTSSVPEYVYNLHLVENDFVGGRSPVPKTYDMLKAGTTATYEGRWGRGTAQYSSQKSVEERSLRHPLRRLEISPDSSPERAHYTHSDYQYSQRSQAGHTLHHQESRRAALLVPPRYARSEIVGVSRAGTTSRQRHFDTYHRQYQHGSVSDTVFDSIPANPALLTYPRPGTSRSMGNLLEKENYLTAGLTVGQVRPLVPLQPVTQNRASRSSWHQSSFHSTRTLREAGPSVAVDSSGRRAHLTVGQAAAGGSGNLLTERSTFTDSQLGNADMEMTLERAVSMLEADHMLPSRISAAATFIQHECFQKSEARKRVNQLRGILKLLQLLKVQNEDVQRAVCGALRNLVFEDNDNKLEVAELNGVPRLLQVLKQTRDLETKKQITDHTVNLRSRNGWPGAVAHACNPSTLGGQGGRITRSGVRDQPDQHGLLWNLSSNDKLKNLMITEALLTLTENIIIPFSGWPEGDYPKANGLLDFDIFYNVTGCLRNMSSAGADGRKAMRRCDGLIDSLVHYVRGTIADYQPDDKATENCVCILHNLSYQLEAELPEKYSQNIYIQNRNIQTDNNKSIGCFGSRSRKVKEQYQDVPMPEEKSNPKGVEWLWHSIVIRMYLSLIAKSVRNYTQEASLGALQNLTAGSGPMPTSVAQTVVQKESGLQHTRKMLHVGDPSVKKTAISLLRNLSRNLSLQNEIAKETLPDLVSIIPDTVPSTDLLIETTASACYTLNNIIQNSYQNARDLLNTGGIQKIMAISAGDAYASNKASKAASVLLYSLWAHTELHHAYKKAQFKKTDFVNSRTAKAYHSLKD

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
100
Pig
92
Horse
92
Bovine
92
Sheep
83
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99959 基板として

Site PTM Type Enzyme
Y10 Phosphorylation
Y12 Phosphorylation
K37 Ubiquitination
K39 Methylation
K39 Ubiquitination
S43 Phosphorylation
S44 Phosphorylation
R46 Methylation
K52 Methylation
K52 Ubiquitination
S53 Phosphorylation
T63 Phosphorylation
S71 Phosphorylation
T80 Phosphorylation
S81 Phosphorylation
S82 Phosphorylation
Y86 Phosphorylation
Y88 Phosphorylation
S102 Phosphorylation
K106 Ubiquitination
T107 Phosphorylation
Y108 Phosphorylation
K112 Methylation
K112 Ubiquitination
T118 Phosphorylation
Y119 Phosphorylation
R125 Methylation
T127 Phosphorylation
Y130 Phosphorylation
S131 Phosphorylation
S132 Phosphorylation
K134 Ubiquitination
S135 Phosphorylation
S140 Phosphorylation
S151 Phosphorylation
S154 Phosphorylation
S155 Phosphorylation
Y161 Phosphorylation
T162 Phosphorylation
S164 Phosphorylation
Y166 Phosphorylation
Y168 Phosphorylation
S169 Phosphorylation
S172 Phosphorylation
T177 Phosphorylation
S183 Phosphorylation
Y194 Phosphorylation
S197 Phosphorylation
S203 Phosphorylation
R204 Methylation
T207 Phosphorylation
T208 Phosphorylation
T216 Phosphorylation
Y217 Phosphorylation
Y221 Phosphorylation
S225 Phosphorylation
S233 Phosphorylation
T242 Phosphorylation
Y243 Phosphorylation
T248 Phosphorylation
S251 Phosphorylation
K258 Ubiquitination
Y261 Phosphorylation
T263 Phosphorylation
T267 Phosphorylation
S288 Phosphorylation
S289 Phosphorylation
S293 Phosphorylation
S294 Phosphorylation
S297 Phosphorylation
R302 Methylation
S312 Phosphorylation
S329 Phosphorylation
S342 Phosphorylation
S358 Phosphorylation
R390 Methylation
R395 Methylation
K405 Ubiquitination
K430 Ubiquitination
K447 Ubiquitination
T459 Phosphorylation
T462 Phosphorylation
Y597 Phosphorylation
Y616 Phosphorylation
Y626 Phosphorylation
Y631 Phosphorylation
K643 Ubiquitination
S650 Phosphorylation
K656 Ubiquitination
Y659 Phosphorylation
Y686 Phosphorylation
S693 Phosphorylation
K736 Ubiquitination
S744 Phosphorylation
K747 Ubiquitination
S761 Phosphorylation
Y831 Phosphorylation
S833 Phosphorylation
K864 Ubiquitination
S870 Phosphorylation
K874 Ubiquitination
Y876 Phosphorylation
S878 Phosphorylation

研究背景

機能:

May play a role in junctional plaques.

細胞の位置付け:

Nucleus. Cell junction>Desmosome.
Note: Nuclear and associated with desmosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in heart right ventricle (at protein level). Widely expressed. Found at desmosomal plaques in simple and stratified epithelia and in non-epithelial tissues such as myocardium and lymph node follicles. In most stratified epithelia found in the desmosomes of the basal cell layer and seems to be absent from suprabasal strata.

サブユニット構造:

Interacts with DSC2. Interacts with JUP and desmoplakin/DSP.

タンパク質ファミリー:

Belongs to the beta-catenin family.

研究領域

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

参考文献

1). Identification of PKP 2/3 as potential biomarkers of ovarian cancer based on bioinformatics and experiments. Cancer Cell International, 2020 (PubMed: 33088217) [IF=5.8]

Application: IHC    Species: Human    Sample: ovarian tissue

Fig. 2 The expression and prognostic values of PKP1/2/3 protein in ovarian cancer (Human Protein Atlas and Kaplan–Meier plotter). A The expression of PKP1 (a), PKP2 (b) and PKP3 (c) in OC and normal tissues stained by immunohistochemistry. B (a–c) Relationship between the expression of PKP1 (a), PKP2(b), PKP3(c) and OS in patients with OC; (d–f) Relationship between the expression of PKP1 (d), PKP2 (e), PKP3( f) and PFS in patients with OC; (g–i) Relationship between the expression of PKP1 (g), PKP2 (h), PKP3 (i) and OS in OC patients with TP53 mutation; (j–l) Relationship between the expression of PKP1 (j), PKP2 (k), PKP3 (l) and PFS in OC patients with TP53 mutation. PKP plakophilin, OC ovarian cancer, OS Overall Survival, PFS progression-free survival

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