RAB11FIP2 Antibody - #DF4370
| 製品: | RAB11FIP2 Antibody |
| カタログ: | DF4370 |
| タンパク質の説明: | Rabbit polyclonal antibody to RAB11FIP2 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 58 KD; 58kD(Calculated). |
| ユニプロット: | Q7L804 |
| RRID: | AB_2836658 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4370, RRID:AB_2836658.
折りたたみ／展開
KIAA0941; NRip11; RAB11 family interacting protein 2 (class I); Rab11 family-interacting protein 2; RAB11 FIP2 long isoform; Rab11-FIP2; RAB11FIP2; RFIP2; RFIP2_HUMAN;
免疫原
- Q7L804 RFIP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMLSEQAQKWFPTHVQVTVLQAKDLKPKGKSGTNDTYTIIQLGKEKYSTSVAEKTLEPVWKEEASFELPGLLIQGSPEKYILFLIVMHRSLVGLDKFLGQVAINLNDIFEDKQRRKTEWFRLESKQGKRIKNRGEIKVNIQFMRNNMTASMFDLSMKDKTRSPFAKLKDKMKGRKNDGTFSDTSSAIIPSTHMPDANSEFSSGEIQMKSKPKKPFLLGPQRLSSAHSMSDLSGSHMSSEKLKAGTIGQTHLLGHQLDSFGTVPESGSLKSPHRRTLSFDTSKMNQPDSIVDEGELCFGRQNDPFTNVTASLPQKFATLPRKKNPFEESSETWDSSMNLFSKPIEIRKENKREKREKVSLFERVTGKKDSRRSDKLNNGGSDSPCDLKSPNAFSENRQDYFDYESTNPFTAKFRASNIMPSSSFHMSPTSNEDLRKIPDSNPFDATAGYRSLTYEEVLQELVKHKELLRRKDTHIRELEDYIDNLLVRVMEETPSILRVPYEPSRKAGKFSNS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q7L804 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T13 | Phosphorylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| T148 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Q7KZI7 (MARK2) | Uniprot |
| S229 | Phosphorylation | Uniprot | |
| T245 | Phosphorylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| T261 | Phosphorylation | Uniprot | |
| T275 | Phosphorylation | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| T280 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| T317 | Phosphorylation | Uniprot | |
| K322 | Ubiquitination | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| K350 | Acetylation | Uniprot | |
| S382 | Phosphorylation | Uniprot | |
| K387 | Methylation | Uniprot | |
| S388 | Phosphorylation | Uniprot | |
| Y399 | Phosphorylation | Uniprot | |
| S429 | Phosphorylation | Uniprot | |
| Y480 | Phosphorylation | Uniprot | |
| S494 | Phosphorylation | Uniprot | |
| Y500 | Phosphorylation | Uniprot |
研究背景
A Rab11 effector binding preferentially phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and phosphatidic acid (PA) and acting in the regulation of the transport of vesicles from the endosomal recycling compartment (ERC) to the plasma membrane. Involved in insulin granule exocytosis. Also involved in receptor-mediated endocytosis and membrane trafficking of recycling endosomes, probably originating from clathrin-coated vesicles. Required in a complex with MYO5B and RAB11 for the transport of NPC1L1 to the plasma membrane. Also acts as a regulator of cell polarity. Plays an essential role in phagocytosis through a mechanism involving TICAM2, RAC1 and CDC42 Rho GTPases for controlling actin-dynamics.
Phosphorylation at Ser-227 by MARK2 regulates epithelial cell polarity.
Cell projection>Phagocytic cup. Cell membrane>Peripheral membrane protein. Recycling endosome membrane>Peripheral membrane protein.
Note: Translocates with RAB11A from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane.
Homooligomerizes in a Rab11-independent manner. Forms a heterooligomeric complex with RAB11FIP4. Interacts with AP2A1, MYO5B, RAB25 and REPS1. Interacts with RAB11A and RAB11B (activated GTP-bound form). Interacts with NPC1L1. Interacts (via NPF motifs) with EHD1 and EHD3. Interacts with TICAM2; this interaction directs RAB11FIP2 to the phagosome.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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