SF3B14 Antibody - #DF4325
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4325, RRID:AB_2836693.
折りたたみ／展開
Ht006; P14; PM14_HUMAN; Pre mRNA branch site protein p14; Pre-mRNA branch site protein p14; SAP14; SF3B 14 kDa subunit; SF3B14; SF3B14a; Splicing factor 3B, 14 kDa subunit;
免疫原
- Q9Y3B4 SF3B6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAMQAAKRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYNANRAFQKMDTKKKEEQLKLLKEKYGINTDPPK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y3B4 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K7 | Sumoylation | Uniprot | |
| K7 | Ubiquitination | Uniprot | |
| K29 | Acetylation | Uniprot | |
| K29 | Ubiquitination | Uniprot | |
| Y36 | Phosphorylation | Uniprot | |
| K41 | Acetylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| Y61 | Phosphorylation | Uniprot | |
| Y64 | Phosphorylation | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| C74 | S-Nitrosylation | Uniprot | |
| C83 | S-Nitrosylation | Uniprot | |
| Y86 | Phosphorylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| K111 | Ubiquitination | Uniprot | |
| K116 | Acetylation | Uniprot | |
| K116 | Ubiquitination | Uniprot |
研究背景
Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.
Nucleus.
Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A and DDX42. Within the SF3B complex interacts directly with SF3B1 Ref.20). The SF3B complex composed of SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6 and PHF5A interacts with U2AF2. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome.
Belongs to the SF3B6 family.
研究領域
· Genetic Information Processing > Transcription > Spliceosome.
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