PPP1R3C Antibody - #DF4346
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4346, RRID:AB_2836714.
折りたたみ／展開
OTTHUMP00000020089; Phosphatase 1, regulatory inhibitor subunit 5; PP1 subunit R5; ppp1r3c; PPP1R5; PPR3C_HUMAN; Protein phosphatase 1 regulatory subunit 3C; Protein phosphatase 1 regulatory subunit 5; protein phosphatase 1, regulatory (inhibitor) subunit 3C; Protein targeting to glycogen; PTG; R5;
免疫原
- Q9UQK1 PPR3C_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSCTRMIQVLDPRPLTSSVMPVDVAMRLCLAHSPPVKSFLGPYDEFQRRHFVNKLKPLKSCLNIKHKAKSQNDWKCSHNQAKKRVVFADSKGLSLTAIHVFSDLPEEPAWDLQFDLLDLNDISSALKHHEEKNLILDFPQPSTDYLSFRSHFQKNFVCLENCSLQERTVTGTVKVKNVSFEKKVQIRITFDSWKNYTDVDCVYMKNVYGGTDSDTFSFAIDLPPVIPTEQKIEFCISYHANGQVFWDNNDGQNYRIVHVQWKPDGVQTQMAPQDCAFHQTSPKTELESTIFGSPRLASGLFPEWQSWGRMENLASYR
PTMs - Q9UQK1 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S33 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| K65 | Ubiquitination | Uniprot | |
| K69 | Acetylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| K82 | Acetylation | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| T289 | Phosphorylation | Uniprot | |
| S293 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
研究背景
Acts as a glycogen-targeting subunit for PP1 and regulates its activity. Activates glycogen synthase, reduces glycogen phosphorylase activity and limits glycogen breakdown. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in a variety of cell types.
Ubiquitinated by NHLRC1/malin in a EPM2A/laforin-dependent manner.
Interacts with PPP1CC catalytic subunit of PP1 and associates with glycogen. Forms complexes with glycogen phosphorylase, glycogen synthase and phosphorylase kinase which is necessary for its regulation of PP1 activity. Also interacts with EPM2A/laforin.
The N-terminal region is required for binding to PP1, the central region is required for binding to glycogen and the C-terminal region is required for binding to glycogen phosphorylase, glycogen synthase and phosphorylase kinase.
研究領域
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
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