PDHA1 Antibody - #DF4367
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4367, RRID:AB_2836735.
折りたたみ／展開
ODPA_HUMAN; PDH; PDHA; PDHA1; PDHCE1A; PDHE1 A type I; PDHE1-A type I; PHE1A; Pyruvate Dehydrogenase (lipoamide) alpha 1; Pyruvate dehydrogenase complex, E1 alpha polypeptide 1; Pyruvate Dehydrogenase E1 alpha; Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial;
免疫原
- P08559 ODPA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRKMLAAVSRVLSGASQKPASRVLVASRNFANDATFEIKKCDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P08559 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K18 | Acetylation | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| K39 | Acetylation | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| K63 | Acetylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| K77 | Acetylation | Uniprot | |
| K83 | Acetylation | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| T116 | Phosphorylation | Uniprot | |
| Y118 | Phosphorylation | Uniprot | |
| T139 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| Y227 | Phosphorylation | Uniprot | |
| T231 | Phosphorylation | Uniprot | |
| S232 | Phosphorylation | Q15118 (PDK1) , O15530 (PDPK1) , Q15119 (PDK2) | Uniprot |
| T240 | Phosphorylation | Uniprot | |
| Y242 | Phosphorylation | Uniprot | |
| Y243 | Phosphorylation | Uniprot | |
| K244 | Acetylation | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| R245 | Methylation | Uniprot | |
| Y272 | Phosphorylation | Uniprot | |
| K277 | Acetylation | Uniprot | |
| Y289 | Phosphorylation | Uniprot | |
| S293 | Phosphorylation | Q16654 (PDK4) , Q15119 (PDK2) , O15530 (PDPK1) , Q15120 (PDK3) , Q15118 (PDK1) | Uniprot |
| S295 | Phosphorylation | Uniprot | |
| S300 | Phosphorylation | Q15119 (PDK2) , Q15118 (PDK1) , Q16654 (PDK4) , Q15120 (PDK3) , O15530 (PDPK1) | Uniprot |
| Y301 | Phosphorylation | Uniprot | |
| T303 | Phosphorylation | Uniprot | |
| K313 | Acetylation | Uniprot | |
| K321 | Acetylation | Uniprot | |
| K321 | Ubiquitination | Uniprot | |
| K336 | Acetylation | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| Y366 | Phosphorylation | Uniprot | |
| Y369 | Phosphorylation | Uniprot | |
| K385 | Methylation | Uniprot |
研究背景
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme; for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation.
Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3 (By similarity).
Mitochondrion matrix.
Ubiquitous.
Heterotetramer of two PDHA1 and two PDHB subunits. The heterotetramer interacts with DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules.
研究領域
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).
· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Organismal Systems > Endocrine system > Glucagon signaling pathway.
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