RAPGEF3 Antibody - #DF4381

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製品: RAPGEF3 Antibody
カタログ: DF4381
タンパク質の説明: Rabbit polyclonal antibody to RAPGEF3
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 110/100 KD; 104kD(Calculated).
ユニプロット: O95398
RRID: AB_2836736

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(86%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
RAPGEF3 Antibody detects endogenous levels of total RAPGEF3.
RRID:
AB_2836736
引用形式: Affinity Biosciences Cat# DF4381, RRID:AB_2836736.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

bcm910; CAMP GEFI; cAMP regulated guanine nucleotide exchange factor I; CAMPGEFI; CGEF 1; CGEF1; EPA1; Epac 1; EPAC; EPAC1; Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; MGC21410; RAP guanine nucleotide exchange factor; Rap guanine nucleotide exchange factor (GEF) 3; RAP guanine nucleotide exchange factor 3; Rap1 guanine nucleotide exchange factor directly activated by cAMP; RAPGEF3;

免疫原

免疫原:
Uniprot:

O95398(RPGF3_HUMAN) >>Visit HPA database.

遺伝子(ID):
RAPGEF3(10411)
発現特異性:
O95398 RPGF3_HUMAN:

Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney.

タンパク質配列:
MKVGWPGESCWQVGLAVEDSPALGAPRVGALPDVVPEGTLLNMVLRRMHRPRSCSYQLLLEHQRPSCIQGLRWTPLTNSEESLDFSESLEQASTERVLRAGRQLHRHLLATCPNLIRDRKYHLRLYRQCCSGRELVDGILALGLGVHSRSQVVGICQVLLDEGALCHVKHDWAFQDRDAQFYRFPGPEPEPVRTHEMEEELAEAVALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILREDNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERASQGAGPSRPPTPGRNRYTVMSGTPEKILELLLEAMGPDSSAHDPTETFLSDFLLTHRVFMPSAQLCAALLHHFHVEPAGGSEQERSTYVCNKRQQILRLVSQWVALYGSMLHTDPVATSFLQKLSDLVGRDTRLSNLLREQWPERRRCHRLENGCGNASPQMKARNLPVWLPNQDEPLPGSSCAIQVGDKVPYDICRPDHSVLTLQLPVTASVREVMAALAQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEVHELIPHPDQLGPTVGSAEGLDLVSAKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGPRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALAKLSPPVIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHNPVPLSPLRSRVSHLHEDSQVARISTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
86
Chicken
79
Xenopus
67
Zebrafish
54
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95398 基板として

Site PTM Type Enzyme
S82 Phosphorylation
S267 Phosphorylation
K268 Acetylation
T380 Phosphorylation
S494 Phosphorylation
S528 Phosphorylation
S864 Phosphorylation

研究背景

機能:

Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin.

細胞の位置付け:

Endomembrane system.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney.

サブユニット構造:

Interacts with PDE3B.

タンパク質ファミリー:

The DEP domain is involved in membrane localization independent from regulation by cAMP.

研究領域

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Serotonergic synapse.

参考文献

1). Epac1 signaling pathway mediates the damage and apoptosis of inner ear hair cells after noise exposure in a rat model. NEUROSCIENCE, 2021 (PubMed: 33838290) [IF=3.3]

Application: WB    Species: rat    Sample: HEI-OC1 cells

Fig. 6. Expression of Epac1 in HEI-OC1 cells after oligomycin exposure. (A) Representative fluorescent microscopic images showing Epac1 expression in HEI-OC1 cells 12 h after oligomycin exposure; blue: DAPI; scale bar = 20 mm, n = 3. (B) Bar graph representing the intensity of Epac1 signal in HEI-OC1 cells. (C) Western blot analysis of Epac1 and Rap1 expression in HEI-OC1 cells 12 h after oligomycin exposure. b-actin was used as the loading control. The data represent the mean ± SEM, n = 3; *P < 0.05 vs. Control group, #P < 0.05 vs. OA.

Application: IF/ICC    Species: rat    Sample: HEI-OC1 cells

Fig. 6. Expression of Epac1 in HEI-OC1 cells after oligomycin exposure. (A) Representative fluorescent microscopic images showing Epac1 expression in HEI-OC1 cells 12 h after oligomycin exposure; blue: DAPI; scale bar = 20 mm, n = 3. (B) Bar graph representing the intensity of Epac1 signal in HEI-OC1 cells. (C) Western blot analysis of Epac1 and Rap1 expression in HEI-OC1 cells 12 h after oligomycin exposure. b-actin was used as the loading control. The data represent the mean ± SEM, n = 3; *P < 0.05 vs. Control group, #P < 0.05 vs. OA.

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