RIN1 Antibody - #DF4384
| 製品: | RIN1 Antibody |
| カタログ: | DF4384 |
| タンパク質の説明: | Rabbit polyclonal antibody to RIN1 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Horse, Dog |
| 分子量: | 84 KD; 84kD(Calculated). |
| ユニプロット: | Q13671 |
| RRID: | AB_2836739 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4384, RRID:AB_2836739.
折りたたみ／展開
Ras and Rab interactor 1; ras inhibitor 1; ras inhibitor; Ras inhibitor JC99; ras inhibitor RIN1; Ras interaction/interference protein 1; RIN 1;
免疫原
Expressed in all tissues examined with high levels in brain, placenta and pancreas.
- Q13671 RIN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MESPGESGAGSPGAPSPSSFTTGHLAREKPAQDPLYDVPNASGGQAGGPQRPGRVVSLRERLLLTRPVWLQLQANAAAALHMLRTEPPGTFLVRKSNTRQCQALCMRLPEASGPSFVSSHYILESPGGVSLEGSELMFPDLVQLICAYCHTRDILLLPLQLPRAIHHAATHKELEAISHLGIEFWSSSLNIKAQRGPAGGPVLPQLKARSPQELDQGTGAALCFFNPLFPGDLGPTKREKFKRSFKVRVSTETSSPLSPPAVPPPPVPVLPGAVPSQTERLPPCQLLRRESSVGYRVPAGSGPSLPPMPSLQEVDCGSPSSSEEEGVPGSRGSPATSPHLGRRRPLLRSMSAAFCSLLAPERQVGRAAAALMQDRHTAAGQLVQDLLTQVRAGPEPQELQGIRQALSRARAMLSAELGPEKLLSPKRLEHVLEKSLHCSVLKPLRPILAARLRRRLAADGSLGRLAEGLRLARAQGPGAFGSHLSLPSPVELEQVRQKLLQLLRTYSPSAQVKRLLQACKLLYMALRTQEGEGAGADEFLPLLSLVLAHCDLPELLLEAEYMSELLEPSLLTGEGGYYLTSLSASLALLSGLGQAHTLPLSPVQELRRSLSLWEQRRLPATHCFQHLLRVAYQDPSSGCTSKTLAVPPEASIATLNQLCATKFRVTQPNTFGLFLYKEQGYHRLPPGALAHRLPTTGYLVYRRAEWPETQGAVTEEEGSGQSEARSRGEEQGCQGDGDAGVKASPRDIREQSETTAEGGQGQAQEGPAQPGEPEAEGSRAAEE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13671 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S3 | Phosphorylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| K29 | Ubiquitination | Uniprot | |
| Y36 | Phosphorylation | P42684 (ABL2) | Uniprot |
| S42 | Phosphorylation | Uniprot | |
| T65 | Phosphorylation | Uniprot | |
| T90 | Phosphorylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| S210 | Phosphorylation | Uniprot | |
| T218 | Phosphorylation | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| T251 | Phosphorylation | Uniprot | |
| T253 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| S292 | Phosphorylation | Q9BZL6 (PRKD2) , Q15139 (PRKD1) | Uniprot |
| Y295 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| T336 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S351 | Phosphorylation | P98161 (PKD1) , Q15139 (PRKD1) | Uniprot |
| T377 | Phosphorylation | Uniprot | |
| T388 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| S424 | Phosphorylation | Uniprot | |
| K513 | Ubiquitination | Uniprot | |
| S609 | Phosphorylation | Uniprot | |
| S611 | Phosphorylation | Uniprot | |
| Y632 | Phosphorylation | Uniprot | |
| R664 | Methylation | Uniprot | |
| Y676 | Phosphorylation | Uniprot | |
| Y681 | Phosphorylation | Uniprot | |
| R692 | Methylation | Uniprot | |
| T696 | Phosphorylation | Uniprot | |
| Y698 | Phosphorylation | Uniprot | |
| Y701 | Phosphorylation | Uniprot | |
| K742 | Ubiquitination | Uniprot | |
| S744 | Phosphorylation | Uniprot |
研究背景
Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protein for binding to activated Ras. Second, by enhancing signaling from ABL1 and ABL2, which regulate cytoskeletal remodeling. Third, by activating RAB5A, possibly by functioning as a guanine nucleotide exchange factor (GEF) for RAB5A, by exchanging bound GDP for free GTP, and facilitating Ras-activated receptor endocytosis.
Phosphorylated on tyrosine residues by ABL1 and ABL2. Phosphorylation at Ser-351 by PRKD1 induces interaction with 14-3-3 proteins.
Cytoplasm. Membrane. Cytoplasm>Cytoskeleton.
Note: Some amount is membrane-associated.
Expressed in all tissues examined with high levels in brain, placenta and pancreas.
Interacts with the GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). This interaction prevents the association between RAF1 and Ras. Interacts with 14-3-3 proteins YWHAB, YWHAE and YWHAZ when phosphorylated on Ser-351. Interacts with the SH3 domain of ABL1 and ABL2. Interacts with RAB5A. The interaction with Ras is probably regulated and antagonized by the interaction with 14-3-3 proteins. The interaction with 14-3-3 proteins is regulated by phosphorylation on Ser-351.
Belongs to the RIN (Ras interaction/interference) family.
研究領域
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.