IQGAP1 Antibody - #DF4388

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製品: IQGAP1 Antibody
カタログ: DF4388
タンパク質の説明: Rabbit polyclonal antibody to IQGAP1
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 190 KD; 189kD(Calculated).
ユニプロット: P46940
RRID: AB_2836743

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(92%), Bovine(83%), Sheep(85%), Rabbit(100%), Dog(92%)
クローナリティ:
Polyclonal
特異性:
IQGAP1 Antibody detects endogenous levels of total IQGAP1.
RRID:
AB_2836743
引用形式: Affinity Biosciences Cat# DF4388, RRID:AB_2836743.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

HUMORFA01; IQ motif containing GTPase activating protein 1; IQGA1_HUMAN; Iqgap1; KIAA0051; p195; Ras GTPase activating like protein 1; Ras GTPase-activating-like protein IQGAP1; RasGAP-like with IQ motifs; SAR1;

免疫原

免疫原:
Uniprot:

P46940(IQGA1_HUMAN) >>Visit HPA database.

遺伝子(ID):
IQGAP1(8826)
発現特異性:
P46940 IQGA1_HUMAN:

Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas.

タンパク質配列:
MSAADEVDGLGVARPHYGSVLDNERLTAEEMDERRRQNVAYEYLCHLEEAKRWMEACLGEDLPPTTELEEGLRNGVYLAKLGNFFSPKVVSLKKIYDREQTRYKATGLHFRHTDNVIQWLNAMDEIGLPKIFYPETTDIYDRKNMPRCIYCIHALSLYLFKLGLAPQIQDLYGKVDFTEEEINNMKTELEKYGIQMPAFSKIGGILANELSVDEAALHAAVIAINEAIDRRIPADTFAALKNPNAMLVNLEEPLASTYQDILYQAKQDKMTNAKNRTENSERERDVYEELLTQAEIQGNINKVNTFSALANIDLALEQGDALALFRALQSPALGLRGLQQQNSDWYLKQLLSDKQQKRQSGQTDPLQKEELQSGVDAANSAAQQYQRRLAAVALINAAIQKGVAEKTVLELMNPEAQLPQVYPFAADLYQKELATLQRQSPEHNLTHPELSVAVEMLSSVALINRALESGDVNTVWKQLSSSVTGLTNIEEENCQRYLDELMKLKAQAHAENNEFITWNDIQACVDHVNLVVQEEHERILAIGLINEALDEGDAQKTLQALQIPAAKLEGVLAEVAQHYQDTLIRAKREKAQEIQDESAVLWLDEIQGGIWQSNKDTQEAQKFALGIFAINEAVESGDVGKTLSALRSPDVGLYGVIPECGETYHSDLAEAKKKKLAVGDNNSKWVKHWVKGGYYYYHNLETQEGGWDEPPNFVQNSMQLSREEIQSSISGVTAAYNREQLWLANEGLITRLQARCRGYLVRQEFRSRMNFLKKQIPAITCIQSQWRGYKQKKAYQDRLAYLRSHKDEVVKIQSLARMHQARKRYRDRLQYFRDHINDIIKIQAFIRANKARDDYKTLINAEDPPMVVVRKFVHLLDQSDQDFQEELDLMKMREEVITLIRSNQQLENDLNLMDIKIGLLVKNKITLQDVVSHSKKLTKKNKEQLSDMMMINKQKGGLKALSKEKREKLEAYQHLFYLLQTNPTYLAKLIFQMPQNKSTKFMDSVIFTLYNYASNQREEYLLLRLFKTALQEEIKSKVDQIQEIVTGNPTVIKMVVSFNRGARGQNALRQILAPVVKEIMDDKSLNIKTDPVDIYKSWVNQMESQTGEASKLPYDVTPEQALAHEEVKTRLDSSIRNMRAVTDKFLSAIVSSVDKIPYGMRFIAKVLKDSLHEKFPDAGEDELLKIIGNLLYYRYMNPAIVAPDAFDIIDLSAGGQLTTDQRRNLGSIAKMLQHAASNKMFLGDNAHLSIINEYLSQSYQKFRRFFQTACDVPELQDKFNVDEYSDLVTLTKPVIYISIGEIINTHTLLLDHQDAIAPEHNDPIHELLDDLGEVPTIESLIGESSGNLNDPNKEALAKTEVSLTLTNKFDVPGDENAEMDARTILLNTKRLIVDVIRFQPGETLTEILETPATSEQEAEHQRAMQRRAIRDAKTPDKMKKSKSVKEDSNLTLQEKKEKIQTGLKKLTELGTVDPKNKYQELINDIARDIRNQRRYRQRRKAELVKLQQTYAALNSKATFYGEQVDYYKSYIKTCLDNLASKGKVSKKPREMKGKKSKKISLKYTAARLHEKGVLLEIEDLQVNQFKNVIFEISPTEEVGDFEVKAKFMGVQMETFMLHYQDLLQLQYEGVAVMKLFDRAKVNVNLLIFLLNKKFYGK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
92
Dog
92
Sheep
85
Bovine
83
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P46940 基板として

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
Y17 Phosphorylation
S19 Phosphorylation
K51 Ubiquitination
Y77 Phosphorylation
S86 Phosphorylation
K88 Ubiquitination
S91 Phosphorylation
Y96 Phosphorylation
K104 Ubiquitination
K130 Ubiquitination
Y133 Phosphorylation
Y140 Phosphorylation
Y172 Phosphorylation
K174 Ubiquitination
T178 Phosphorylation
K186 Ubiquitination
S200 Phosphorylation
S211 Phosphorylation
K266 Ubiquitination
S330 Phosphorylation
R336 Methylation
K348 Acetylation
K348 Ubiquitination
K354 Ubiquitination
S360 Phosphorylation
K401 Ubiquitination
K406 Ubiquitination
T435 Phosphorylation
K503 Ubiquitination
K556 Ubiquitination
T557 Phosphorylation
Y579 Phosphorylation
S648 Phosphorylation
Y654 Phosphorylation
T663 Phosphorylation
K672 Ubiquitination
K684 Acetylation
K684 Ubiquitination
K687 Ubiquitination
Y694 Phosphorylation
Y695 Phosphorylation
Y697 Phosphorylation
S730 Phosphorylation
Y736 Phosphorylation
R787 Methylation
R798 Methylation
K811 Ubiquitination
S814 Phosphorylation
Y831 Phosphorylation
Y855 Phosphorylation
K856 Ubiquitination
T857 Phosphorylation
S879 Phosphorylation
S902 Phosphorylation
K916 Ubiquitination
K922 Methylation
K922 Ubiquitination
K924 Ubiquitination
S932 Phosphorylation
S934 Phosphorylation
K935 Ubiquitination
K942 Methylation
K942 Ubiquitination
K953 Ubiquitination
K959 Acetylation
K959 Ubiquitination
S962 Phosphorylation
K963 Acetylation
K965 Acetylation
K997 Ubiquitination
Y1020 Phosphorylation
K1027 Acetylation
K1027 Ubiquitination
T1028 Phosphorylation
K1035 Ubiquitination
K1037 Ubiquitination
S1057 Phosphorylation
K1083 Ubiquitination
K1088 Acetylation
K1088 Methylation
K1088 Ubiquitination
Y1095 Phosphorylation
K1096 Methylation
K1096 Ubiquitination
S1097 Phosphorylation
K1111 Ubiquitination
Y1114 Phosphorylation
K1128 Ubiquitination
S1133 Phosphorylation
R1136 Methylation
K1144 Acetylation
K1144 Ubiquitination
S1147 Phosphorylation
S1151 Phosphorylation
S1152 Phosphorylation
K1155 Ubiquitination
Y1158 Phosphorylation
K1165 Ubiquitination
K1168 Ubiquitination
K1174 Ubiquitination
T1218 Phosphorylation
S1227 Phosphorylation
K1230 Acetylation
K1230 Ubiquitination
S1237 Phosphorylation
R1263 Methylation
R1264 Methylation
Y1284 Phosphorylation
S1285 Phosphorylation
T1359 Phosphorylation
K1389 Ubiquitination
T1403 Phosphorylation
T1434 Phosphorylation
S1441 Phosphorylation
K1442 Ubiquitination
S1443 Phosphorylation P17252 (PRKCA) , Q02156 (PRKCE)
K1445 Acetylation
K1445 Ubiquitination
S1448 Phosphorylation
T1451 Phosphorylation
K1464 Ubiquitination
K1465 Ubiquitination
T1467 Phosphorylation
K1475 Ubiquitination
K1477 Acetylation
K1477 Ubiquitination
Y1478 Phosphorylation
K1505 Ubiquitination
T1509 Phosphorylation
Y1510 Phosphorylation
S1515 Phosphorylation
K1516 Ubiquitination
Y1526 Phosphorylation
Y1527 Phosphorylation
K1528 Acetylation
K1528 Ubiquitination
K1532 Acetylation
K1532 Ubiquitination
C1534 S-Nitrosylation
S1540 Phosphorylation
K1541 Ubiquitination
K1547 Acetylation
K1552 Acetylation
K1555 Acetylation
K1558 Acetylation
K1562 Acetylation
K1562 Ubiquitination
K1571 Acetylation
K1571 Ubiquitination
K1604 Ubiquitination
Y1655 Phosphorylation

研究背景

機能:

Plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth. May play a possible role in cell cycle regulation by contributing to cell cycle progression after DNA replication arrest.

PTMs:

Phosphorylation of Ser-1443 by PKC/PRKCE prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth.

細胞の位置付け:

Cell membrane. Nucleus. Cytoplasm.
Note: Subcellular distribution is regulated by the cell cycle, nuclear levels increase at G1/S phase (PubMed:20883816).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas.

サブユニット構造:

Interacts with CDC42; the interaction is demonstrated with IQGAP1 in GTP-bound and in nucleotide-free state. Interacts with RAC1. Does not interact with RHOA. Interacts with TSG101. Interacts with PAK6. Interacts with TMEM14B; this interaction increases IQGAP1 phosphorylation and induces its nuclear translocation. Interacts with SASH1.

(Microbial infection) Interacts with ebolavirus vp40.

タンパク質ファミリー:

Regions C1 and C2 can either interact with nucleotide-free CDC42, or interact together, depending on the phosphorylation state of Ser-1443. When Ser-1443 is not phosphorylated, C1 and C2 interact, which prevents binding of nucleotide-free CDC42 and promotes binding of GTP-bound CDC42. Phosphorylation of Ser-1443 prevents interaction between C1 and C2, which opens the structure of the C-terminus and allows binding and sequestration of nucleotide-free CDC42 on both C1 and C2.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

参考文献

1). The mechanism of SP1/p300 complex promotes proliferation of multiple myeloma cells through regulating IQGAP1 transcription. BIOMEDICINE & PHARMACOTHERAPY, 2019 (PubMed: 31536933) [IF=7.5]

Application: WB    Species: human    Sample: myeloma cell

Fig. 2.| A. The mRNA expression level of IQGAP1 in myeloma cell lines among different group (Sp1-siRNA: p＜0.0001; p300-siRNA: p = 0.0003；pcDNA3.1-Sp1: p＜0.0001; pcDNA3.1- p300: p＜0.0001). B. The mRNA expression level of Sp1 in myeloma cell lines among different group (Sp1-siRNA: p = 0.0014; pcDNA3.1-Sp1:p = 0.0001; pcDNA3.1- p300: p = 0.0006). C. The mRNA expression level of p300 in myeloma cell lines among different group (p300-siRNA: p = 0.0012; pcDNA3.1-Sp1: p = 0.0003; pcDNA3.1- p300: p = 0.0002). D. The protein level of Sp1, p300, IQGAP1, ERK1/2 and p-ERK1/2 in myeloma cell lines among different group.

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