RRAGA/B Antibody - #DF4392
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製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4392, RRID:AB_2836747.
折りたたみ／展開
Adenovirus E3 14.7 kDa-interacting protein 1; FIP-1; Rag A; RagA; Ras-related GTP-binding protein A; RRAGA; RRAGA_HUMAN; Adenovirus E3 14.7 kDa-interacting protein 1; FIP1; Rag A; RAg B; RagA; RAGB; Ras related GTP binding protein A; Ras-related GTP binding protein B;
免疫原
Ubiquitously expressed with highest levels of expression in skeletal muscle, heart, and brain.
- Q7L523 RRAGA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPNTAMKKKVLLMGKSGSGKTSMRSIIFANYIARDTRRLGATIDVEHSHVRFLGNLVLNLWDCGGQDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNSPDAKIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECACFRTSIWDETLYKAWSSIVYQLIPNVQQLEMNLRNFAQIIEADEVLLFERATFLVISHYQCKEQRDVHRFEKISNIIKQFKLSCSKLAASFQSMEVRNSNFAAFIDIFTSNTYVMVVMSDPSIPSAATLINIRNARKHFEKLERVDGPKHSLLMR
- Q5VZM2 RRAGB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEESDSEKTTEKENLGPRMDPPLGEPEGSLGWVLPNTAMKKKVLLMGKSGSGKTSMRSIIFANYIARDTRRLGATILDRIHSLQINSSLSTYSLVDSVGNTKTFDVEHSHVRFLGNLVLNLWDCGGQDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNSPDAKIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECSCFRTSIWDETLYKAWSSIVYQLIPNVQQLEMNLRNFAEIIEADEVLLFERATFLVISHYQCKEQRDAHRFEKISNIIKQFKLSCSKLAASFQSMEVRNSNFAAFIDIFTSNTYVMVVMSDPSIPSAATLINIRNARKHFEKLERVDGPKQCLLMR
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q7L523/Q5VZM2 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K15 | Ubiquitination | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| T21 | Phosphorylation | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| Y31 | Phosphorylation | Uniprot | |
| T36 | Phosphorylation | Uniprot | |
| T42 | Phosphorylation | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K236 | Ubiquitination | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| S309 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| Y64 | Phosphorylation | Uniprot | |
| T69 | Phosphorylation | Uniprot | |
| S157 | Phosphorylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| K203 | Ubiquitination | Uniprot | |
| Y278 | Phosphorylation | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K297 | Acetylation | Uniprot | |
| K297 | Ubiquitination | Uniprot | |
| K300 | Acetylation | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| K305 | Acetylation | Uniprot | |
| K305 | Ubiquitination | Uniprot | |
| K360 | Ubiquitination | Uniprot |
研究背景
Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGC or RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death.
Ubiquitinated. 'Lys-68'-linked polyubiquitination of the GDP-bound inactive form of RRAGA by RNF152 is increased in response to amino acid starvation. Polyubiquitination promotes interaction with the GATOR1 complex. This does not affect RRAGA degradation.
Cytoplasm. Nucleus. Lysosome.
Note: Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state (PubMed:8995684, PubMed:9394008). Colocalizes in vivo with adenovirus E3-14.7K mainly to the cytoplasm especially near the nuclear membrane and in discrete foci on or near the plasma membrane (PubMed:8995684).
Ubiquitously expressed with highest levels of expression in skeletal muscle, heart, and brain.
Can occur as a homodimer or as a heterodimer with RRAGC or RRAGD in a sequence-independent manner; heterodimerization stabilizes proteins of the heterodimer. In complex with RRAGC, but not with RRAGB, interacts with RPTOR. The GTP-bound form of RRAGA interacts with NOL8. Interacts with SH3BP4; the interaction with this negative regulator is most probably direct, preferentially occurs with the inactive GDP-bound form of RRAGA and is negatively regulated by amino acids. The Rag heterodimer interacts with SLC38A9; the probable amino acid sensor. Interacts (inactive GDP-bound form) with RNF152; stimulated by amino acid starvation. Interacts (polyubiquitinated) with the GATOR1 complex; inactivates RRAGA. Interacts (polyubiquitinated) with TSC2. Interacts with SESN1, SESN2 AND SESN3. Interacts with PIP4P1 (By similarity). Interacts with GPR137B.
(Microbial infection) Interacts with adenovirus E3 14.7 kDa protein.
Belongs to the GTR/RAG GTP-binding protein family.
Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGC or RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway.
Cytoplasm. Lysosome.
Interacts with RRAGC and RRAGD; heterodimerization stabilizes RRAG proteins. In complex with RRAGC, but not with RRAGA, interacts with RPTOR; this interaction is particularly efficient with GTP-loaded RRAGB and GDP-loaded RRAGC. Interacts with SH3BP4; the interaction with this negative regulator is most probably direct, preferentially occurs with the inactive GDP-bound form of RRAGB, is negatively regulated by amino acids and prevents interaction with RPTOR. Interacts with the GATOR1 complex; inactivates RRAGB. The Rag heterodimer interacts with SLC38A9; the probable amino acid sensor. Interacts with SESN1, SESN2 AND SESN3.
Belongs to the GTR/RAG GTP-binding protein family.
研究領域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
参考文献
Application: WB Species: Rat Sample: tumour cells
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