RRAGB Antibody - #DF4393
| 製品: | RRAGB Antibody |
| カタログ: | DF4393 |
| タンパク質の説明: | Rabbit polyclonal antibody to RRAGB |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 43 KD; 43kD(Calculated). |
| ユニプロット: | Q5VZM2 |
| RRID: | AB_2836748 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4393, RRID:AB_2836748.
折りたたみ／展開
Adenovirus E3 14.7 kDa-interacting protein 1; FIP1; Rag A; RAg B; RagA; RAGB; Ras related GTP binding protein A; Ras-related GTP binding protein B;
免疫原
- Q5VZM2 RRAGB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEESDSEKTTEKENLGPRMDPPLGEPEGSLGWVLPNTAMKKKVLLMGKSGSGKTSMRSIIFANYIARDTRRLGATILDRIHSLQINSSLSTYSLVDSVGNTKTFDVEHSHVRFLGNLVLNLWDCGGQDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNSPDAKIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECSCFRTSIWDETLYKAWSSIVYQLIPNVQQLEMNLRNFAEIIEADEVLLFERATFLVISHYQCKEQRDAHRFEKISNIIKQFKLSCSKLAASFQSMEVRNSNFAAFIDIFTSNTYVMVVMSDPSIPSAATLINIRNARKHFEKLERVDGPKQCLLMR
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q5VZM2 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| Y64 | Phosphorylation | Uniprot | |
| T69 | Phosphorylation | Uniprot | |
| S157 | Phosphorylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| K203 | Ubiquitination | Uniprot | |
| Y278 | Phosphorylation | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K297 | Acetylation | Uniprot | |
| K297 | Ubiquitination | Uniprot | |
| K300 | Acetylation | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| K305 | Acetylation | Uniprot | |
| K305 | Ubiquitination | Uniprot | |
| K360 | Ubiquitination | Uniprot |
研究背景
Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGC or RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway.
Cytoplasm. Lysosome.
Interacts with RRAGC and RRAGD; heterodimerization stabilizes RRAG proteins. In complex with RRAGC, but not with RRAGA, interacts with RPTOR; this interaction is particularly efficient with GTP-loaded RRAGB and GDP-loaded RRAGC. Interacts with SH3BP4; the interaction with this negative regulator is most probably direct, preferentially occurs with the inactive GDP-bound form of RRAGB, is negatively regulated by amino acids and prevents interaction with RPTOR. Interacts with the GATOR1 complex; inactivates RRAGB. The Rag heterodimer interacts with SLC38A9; the probable amino acid sensor. Interacts with SESN1, SESN2 AND SESN3.
Belongs to the GTR/RAG GTP-binding protein family.
研究領域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
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