製品: RAB5C Antibody
カタログ: DF4406
タンパク質の説明: Rabbit polyclonal antibody to RAB5C
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
分子量: 30 KD; 23kD(Calculated).
ユニプロット: P51148
RRID: AB_2836761

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(90%)
クローナリティ:
Polyclonal
特異性:
RAB5C Antibody detects endogenous levels of total RAB5C.
RRID:
AB_2836761
引用形式: Affinity Biosciences Cat# DF4406, RRID:AB_2836761.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

L1880; RAB, member of RAS oncogene family like; RAB5C; RAB5C, member of RAS oncogene family; RAB5C, member RAS oncogene family; RAB5C_HUMAN; RAB5CL; RAB5L; RABL; Ras-related protein Rab-5C;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MAGRGGAARPNGPAAGNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNTDTFARAKNWVKELQRQASPNIVIALAGNKADLASKRAVEFQEAQAYADDNSLLFMETSAKTAMNVNEIFMAIAKKLPKNEPQNATGAPGRNRGVDLQENNPASRSQCCSN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
90
Zebrafish
90
Chicken
78
Rabbit
78
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51148 基板として

Site PTM Type Enzyme
R4 Methylation
R9 Methylation
K18 Ubiquitination
K23 Acetylation
S30 Phosphorylation
K34 Ubiquitination
Y83 Phosphorylation
S85 Phosphorylation
K117 Ubiquitination
S124 Phosphorylation
K135 Ubiquitination
S140 Phosphorylation
K141 Ubiquitination
K180 Ubiquitination
S209 Phosphorylation

研究背景

機能:

Protein transport. Probably involved in vesicular traffic (By similarity).

PTMs:

Phosphorylation of Ser-85 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.

細胞の位置付け:

Cell membrane>Lipid-anchor>Cytoplasmic side. Early endosome membrane>Lipid-anchor. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with EEA1. Interacts with INCA1. Interacts with GDI1, GDI2, CHML and CHM; phosphorylation at Ser-85 disrupts this interaction.

タンパク質ファミリー:

Belongs to the small GTPase superfamily. Rab family.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

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