ALDH1A2 Antibody - #DF4422

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製品: ALDH1A2 Antibody
カタログ: DF4422
タンパク質の説明: Rabbit polyclonal antibody to ALDH1A2
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 52 KD; 57kD(Calculated).
ユニプロット: O94788
RRID: AB_2836777

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 100ul $280 在庫あり
 200ul $350 在庫あり

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(90%)
クローナリティ:
Polyclonal
特異性:
ALDH1A2 Antibody detects endogenous levels of total ALDH1A2.
RRID:
AB_2836777
引用形式: Affinity Biosciences Cat# DF4422, RRID:AB_2836777.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AL1A2_HUMAN; Aldehyde dehydrogenase family 1 member A2; ALDH1A2 aldehyde dehydrogenase 1 family, member A2; ALDH1A2; Aldh1a7; AV116159; MGC26444; RALDH 2; RALDH(II); Raldh1; RalDH2; RALDH2 T; Retinal dehydrogenase 2; Retinaldehyde dehydrogenase 2; Retinaldehyde specific dehydrogenase type 2; Retinaldehyde-specific dehydrogenase type 2;

免疫原

免疫原:
Uniprot:

O94788(AL1A2_HUMAN) >>Visit HPA database.

遺伝子(ID):
ALDH1A2(8854)
タンパク質配列:
MTSSKIEMPGEVKADPAALMASLHLLPSPTPNLEIKYTKIFINNEWQNSESGRVFPVYNPATGEQVCEVQEADKADIDKAVQAARLAFSLGSVWRRMDASERGRLLDKLADLVERDRAVLATMESLNGGKPFLQAFYVDLQGVIKTFRYYAGWADKIHGMTIPVDGDYFTFTRHEPIGVCGQIIPWNFPLLMFAWKIAPALCCGNTVVIKPAEQTPLSALYMGALIKEAGFPPGVINILPGYGPTAGAAIASHIGIDKIAFTGSTEVGKLIQEAAGRSNLKRVTLELGGKSPNIIFADADLDYAVEQAHQGVFFNQGQCCTAGSRIFVEESIYEEFVRRSVERAKRRVVGSPFDPTTEQGPQIDKKQYNKILELIQSGVAEGAKLECGGKGLGRKGFFIEPTVFSNVTDDMRIAKEEIFGPVQEILRFKTMDEVIERANNSDFGLVAAVFTNDINKALTVSSAMQAGTVWINCYNALNAQSPFGGFKMSGNGREMGEFGLREYSEVKTVTVKIPQKNS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
90
Zebrafish
80
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O94788 基板として

Site PTM Type Enzyme
S22 Phosphorylation
K108 Ubiquitination
Y168 Phosphorylation
T170 Phosphorylation
T172 Phosphorylation
K269 Ubiquitination
S278 Phosphorylation
T284 Phosphorylation
Y333 Phosphorylation
S351 Phosphorylation
K365 Ubiquitination
K366 Ubiquitination
K370 Ubiquitination
K384 Acetylation
K384 Ubiquitination
K390 Acetylation
K390 Ubiquitination
K395 Ubiquitination
K415 Ubiquitination
K429 Ubiquitination
K507 Ubiquitination
K512 Ubiquitination

研究背景

機能:

Converts retinaldehyde to retinoic acid. Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Can metabolize octanal and decanal, but has only very low activity with benzaldehyde, acetaldehyde and propanal. Displays complete lack of activity with citral (By similarity).

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homotetramer.

タンパク質ファミリー:

Belongs to the aldehyde dehydrogenase family.

研究領域

· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

参考文献

1). Transferrin-dependent crosstalk between the intestinal tract and commensal microbes contributes for immune tolerance. bioRxiv, 2020

Application: WB    Species: Mouse    Sample: gut tissue

Figure 1. Transferrin is a potential contributory factor for intestinal tolerance dependent on gut microbiota. (A) Transferrin concentration in plasma of germ-free (GF) and specific pathogen-free (SPF) (control) mice determined by ELISA. Data represent means ± SD (n = 10), *p < 0.05, **p < 0.01 by one-way ANOVA with Dunnett’s post-hoc test. Western blotting (left) and quantification (right) analyses of transferrin levels in protein extracts from liver (B) and spleen (C) of SPF and GF mice. β-actin was used as the control. Data represent means ± SD (n = 10), *p < 0.05, **p < 0.01 by one-way ANOVA with Dunnett’s post-hoc test (B, C). (D-E) Transferrin, ALDH1A2, CCL22, TGF-β, and IL-10 levels in different gut tissue segments (duodenum, jejunum, ileum, caecum, and colon (D, J, I, C1, and C2)) of SPF (NC), GF (D), and broad-spectrum antibiotic (Abs)-treated SPF (E) mice were determined by Western blotting (left) and quantification (right) analyses. GAPDH was used as the control. Data represent means ± SD (n = 8),

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