RHG17 Antibody - #DF4426
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4426, RRID:AB_2836781.
折りたたみ／展開
MST066; MST110; MSTP038; MSTP066; MSTP110; NADRIN; Neuron associated developmentally regulated protein; PP367; PP4534; Rho GTPase activating protein 17; Rho type GTPase activating protein 17; RhoGAP interacting with CIP4 homologs 1; RhoGAP interacting with CIP4 homologs protein 1; RICH 1; RICH1; RICH1B; WBP15;
免疫原
- Q68EM7 RHG17_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKKQFNRMKQLANQTVGRAEKTEVLSEDLLQIERRLDTVRSICHHSHKRLVACFQGQHGTDAERRHKKLPLTALAQNMQEASTQLEDSLLGKMLETCGDAENQLALELSQHEVFVEKEIVDPLYGIAEVEIPNIQKQRKQLARLVLDWDSVRARWNQAHKSSGTNFQGLPSKIDTLKEEMDEAGNKVEQCKDQLAADMYNFMAKEGEYGKFFVTLLEAQADYHRKALAVLEKTLPEMRAHQDKWAEKPAFGTPLEEHLKRSGREIALPIEACVMLLLETGMKEEGLFRIGAGASKLKKLKAALDCSTSHLDEFYSDPHAVAGALKSYLRELPEPLMTFNLYEEWTQVASVQDQDKKLQDLWRTCQKLPPQNFVNFRYLIKFLAKLAQTSDVNKMTPSNIAIVLGPNLLWARNEGTLAEMAAATSVHVVAVIEPIIQHADWFFPEEVEFNVSEAFVPLTTPSSNHSFHTGNDSDSGTLERKRPASMAVMEGDLVKKESFGVKLMDFQAHRRGGTLNRKHISPAFQPPLPPTDGSTVVPAGPEPPPQSSRAESSSGGGTVPSSAGILEQGPSPGDGSPPKPKDPVSAAVPAPGRNNSQIASGQNQPQAAAGSHQLSMGQPHNAAGPSPHTLRRAVKKPAPAPPKPGNPPPGHPGGQSSSGTSQHPPSLSPKPPTRSPSPPTQHTGQPPGQPSAPSQLSAPRRYSSSLSPIQAPNHPPPQPPTQATPLMHTKPNSQGPPNPMALPSEHGLEQPSHTPPQTPTPPSTPPLGKQNPSLPAPQTLAGGNPETAQPHAGTLPRPRPVPKPRNRPSVPPPPQPPGVHSAGDSSLTNTAPTASKIVTDSNSRVSEPHRSIFPEMHSDSASKDVPGRILLDIDNDTESTAL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q68EM7 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K3 | Methylation | Uniprot | |
| K9 | Ubiquitination | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| K48 | Acetylation | Uniprot | |
| T72 | Phosphorylation | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| T83 | Phosphorylation | Uniprot | |
| Y124 | Phosphorylation | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| K247 | Ubiquitination | Uniprot | |
| K295 | Acetylation | Uniprot | |
| K295 | Ubiquitination | Uniprot | |
| Y314 | Phosphorylation | Uniprot | |
| K325 | Ubiquitination | Uniprot | |
| Y377 | Phosphorylation | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| S474 | Phosphorylation | Uniprot | |
| S484 | Phosphorylation | Uniprot | |
| S497 | Phosphorylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| S560 | Phosphorylation | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| S575 | Phosphorylation | Uniprot | |
| S584 | Phosphorylation | Uniprot | |
| S595 | Phosphorylation | Uniprot | |
| S599 | Phosphorylation | Uniprot | |
| S610 | Phosphorylation | Uniprot | |
| S614 | Phosphorylation | Uniprot | |
| S625 | Phosphorylation | Uniprot | |
| T628 | Phosphorylation | Uniprot | |
| S667 | Phosphorylation | Uniprot | |
| T672 | Phosphorylation | Uniprot | |
| S674 | Phosphorylation | Uniprot | |
| S676 | Phosphorylation | Uniprot | |
| T679 | Phosphorylation | Uniprot | |
| T682 | Phosphorylation | Uniprot | |
| S690 | Phosphorylation | Uniprot | |
| S696 | Phosphorylation | Uniprot | |
| S702 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S703 | Phosphorylation | Uniprot | |
| S704 | Phosphorylation | Uniprot | |
| S706 | Phosphorylation | Uniprot | |
| S751 | Phosphorylation | Uniprot | |
| T753 | Phosphorylation | Uniprot | |
| T757 | Phosphorylation | Uniprot | |
| T759 | Phosphorylation | Uniprot | |
| S762 | Phosphorylation | Uniprot | |
| T763 | Phosphorylation | Uniprot | |
| T793 | Phosphorylation | Uniprot | |
| S808 | Phosphorylation | Uniprot | |
| S857 | Phosphorylation | Uniprot |
研究背景
Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. Specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. Participates in the Ca(2+)-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activator in vitro for RAC1.
Membrane>Peripheral membrane protein. Cytoplasm. Cell junction>Tight junction.
Note: Associates with membranes and concentrates at sites of cell-cell contact.
Ubiquitously expressed. Expressed at higher level in heart and placenta.
Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, PATJ and PARD3/PAR3. Interacts with SLC9A3R1, FNBP1, TRIP10, CAPZA (CAPZA1, CAPZA2 or CAPZA3), CAPZB, CD2AP and SH3KBP1/CIN85.
The BAR domain mediates the interaction with the coiled coil domain of AMOT, leading to its recruitment to tight junctions.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
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