ARHGEF1 Antibody - #DF4429
| 製品: | ARHGEF1 Antibody |
| カタログ: | DF4429 |
| タンパク質の説明: | Rabbit polyclonal antibody to ARHGEF1 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Sheep, Dog |
| 分子量: | 105 KD; 102kD(Calculated). |
| ユニプロット: | Q92888 |
| RRID: | AB_2836784 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4429, RRID:AB_2836784.
折りたたみ／展開
115 kD protein; 115 kDa guanine nucleotide exchange factor; ARHG1_HUMAN; ARHGEF 1; ARHGEF1; GEF 1; GEF1; LBCL 2; LBCL2; LSC; Lsc homolog; p115 RhoGEF; p115-RhoGEF; p115RhoGEF; Rho guanine nucleotide exchange factor (GEF) 1; Rho guanine nucleotide exchange factor 1; Sub1.5;
免疫原
- Q92888 ARHG1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEDFARGAASPGPSRPGLVPVSIIGAEDEDFENELETNSEEQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLLCCLHADMLGSLGPKEAKKAFLDFYHSFLEKTAVLRVPVPPNVAFELDRTRADLISEDVQRRFVQEVVQSQQVAVGRQLEDFRSKRLMGMTPWEQELAQLEAWVGRDRASYEARERHVAERLLMHLEEMQHTISTDEEKSAAVVNAIGLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPAKTKKGLSSILDAARWNRGEPQVPDFRHLKAEVDAEKPGATDRKGGVGMPSRDRNIGAPGQDTPGVSLHPLSLDSPDREPGADAPLELGDSSPQGPMSLESLAPPESTDEGAETESPEPGDEGEPGRSGLELEPEEPPGWRELVPPDTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFFQPMAECLFFPLEELQNIFPSLDELIEVHSLFLDRLMKRRQESGYLIEEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEEPTEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLSHLRQSSDPMLSEFKNLDITKKKLVHEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERLLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCALITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGRETSPADARTERILSDLLPFCRPGPEGQLAATALRKVLSLKQLLFPAEEDNGAGPPRDGDGVPGGGPLSPARTQEIQENLLSLEETMKQLEELEEEFCRLRPLLSQLGGNSVPQPGCT
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q92888 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S10 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| K234 | Acetylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| S374 | Phosphorylation | Uniprot | |
| S409 | Phosphorylation | Uniprot | |
| K412 | Ubiquitination | Uniprot | |
| Y487 | Phosphorylation | Uniprot | |
| K510 | Ubiquitination | Uniprot | |
| K527 | Ubiquitination | Uniprot | |
| K555 | Ubiquitination | Uniprot | |
| K567 | Ubiquitination | Uniprot | |
| K587 | Ubiquitination | Uniprot | |
| S625 | Phosphorylation | Uniprot | |
| S630 | Phosphorylation | Uniprot | |
| S631 | Phosphorylation | Uniprot | |
| K639 | Ubiquitination | Uniprot | |
| K647 | Ubiquitination | Uniprot | |
| K688 | Ubiquitination | Uniprot | |
| T695 | Phosphorylation | Uniprot | |
| K701 | Ubiquitination | Uniprot | |
| Y738 | Phosphorylation | O60674 (JAK2) | Uniprot |
| K763 | Ubiquitination | Uniprot | |
| S776 | Phosphorylation | Uniprot | |
| S786 | Phosphorylation | Uniprot | |
| S788 | Phosphorylation | Uniprot | |
| S798 | Phosphorylation | Uniprot | |
| S809 | Phosphorylation | Uniprot | |
| S833 | Phosphorylation | Uniprot | |
| K835 | Ubiquitination | Uniprot | |
| S863 | Phosphorylation | Uniprot | |
| S876 | Phosphorylation | Uniprot | |
| T912 | Phosphorylation | Uniprot |
研究背景
Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.
Phosphorylated by PKCA. Angiotensin-2 induced Tyr-738 phosphorylation is mediated by JAK2.
Cytoplasm. Membrane.
Note: Translocated to the membrane by activated GNA13 or LPA stimulation.
Ubiquitously expressed.
Interacts with RHOA, GNA12 and GNA13. Homooligomerizes through the coiled coil region. May interact with CCPG1 (By similarity). Interacts with CTNNAL1.
The RGSL domain, also known as rgRGS domain, is necessary but not sufficient for GAP activity.
The DH domain is involved in interaction with CCPG1.
研究領域
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
· Organismal Systems > Immune system > Platelet activation. (View pathway)
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