ARHGEF7 Antibody - #DF4436
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4436, RRID:AB_2836791.
折りたたみ／展開
ARHG7; ARHG7_HUMAN; ARHGEF 7; Arhgef7; Beta pix; Beta-Pix; betaPix; betaPix-b; betaPix-c; Cool; COOL-1; COOL1; DKFZp686C12170; DKFZp761K1021; KIAA0142; KIAA0412; mKIAA0142; Nbla10314; P50; P50BP; p85; P85COOL1; P85SPR; PAK interacting exchange factor beta; PAK-interacting exchange factor beta; PAK3; Pak3bp; PIX; PIXB; Rho guanine nucleotide exchange factor (GEF) 7; Rho guanine nucleotide exchange factor (GEF) 7b; Rho guanine nucleotide exchange factor (GEF7); Rho guanine nucleotide exchange factor 7; SH3 domain containing proline rich protein;
免疫原
- Q14155 ARHG7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNSAEQTVTWLITLGVLESPKKTISDPEGFLQASLKDGVVLCRLLERLLPGTIEKVYPEPRSESECLSNIREFLRGCGASLRLELLFPPSQPPQHLVTTILLSASTFDANDLYQGQNFNKVLSSLVTLNKVTADIGLGSDSVCARPSSHRIKSFDSLGSQSLHTRTSKLFQGQYRSLDMTDNSNNQLVVRAKFNFQQTNEDELSFSKGDVIHVTRVEEGGWWEGTLNGRTGWFPSNYVREVKASEKPVSPKSGTLKSPPKGFDTTAINKSYYNVVLQNILETENEYSKELQTVLSTYLRPLQTSEKLSSANISYLMGNLEEICSFQQMLVQSLEECTKLPEAQQRVGGCFLNLMPQMKTLYLTYCANHPSAVNVLTEHSEELGEFMETKGASSPGILVLTTGLSKPFMRLDKYPTLLKELERHMEDYHTDRQDIQKSMAAFKNLSAQCQEVRKRKELELQILTEAIRNWEGDDIKTLGNVTYMSQVLIQCAGSEEKNERYLLLFPNVLLMLSASPRMSGFIYQGKLPTTGMTITKLEDSENHRNAFEISGSMIERILVSCNNQQDLQEWVEHLQKQTKVTSVGNPTIKPHSVPSHTLPSHPVTPSSKHADSKPAPLTPAYHTLPHPSHHGTPHTTINWGPLEPPKTPKPWSLSCLRPAPPLRPSAALCYKEDLSKSPKTMKKLLPKRKPERKPSDEEFASRKSTAALEEDAQILKVIEAYCTSAKTRQTLNSTWQGTDLMHNHVLADDDQPSLDSLGRRSSLSRLEPSDLSEDSDYDSIWTAHSYRMGSTSRKSCCSYISHQN
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14155 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S25 | Phosphorylation | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| T127 | Phosphorylation | Q5S007 (LRRK2) | Uniprot |
| S139 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| T164 | Phosphorylation | Q5S007 (LRRK2) | Uniprot |
| S176 | Phosphorylation | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S249 | Phosphorylation | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| T254 | Phosphorylation | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| T264 | Phosphorylation | Uniprot | |
| K405 | Ubiquitination | Uniprot | |
| K418 | Ubiquitination | Uniprot | |
| Y427 | Phosphorylation | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| S518 | Phosphorylation | P05129 (PRKCG) , Q13153 (PAK1) | Uniprot |
| T528 | Phosphorylation | Uniprot | |
| S539 | Phosphorylation | Uniprot | |
| K575 | Ubiquitination | Uniprot | |
| T580 | Phosphorylation | Uniprot | |
| S581 | Phosphorylation | Uniprot | |
| S599 | Phosphorylation | Uniprot | |
| T603 | Phosphorylation | Uniprot | |
| T617 | Phosphorylation | Uniprot | |
| Y620 | Phosphorylation | Uniprot | |
| S653 | Phosphorylation | Uniprot | |
| K675 | Acetylation | Uniprot | |
| S676 | Phosphorylation | Uniprot | |
| K678 | Acetylation | Uniprot | |
| K681 | Acetylation | Uniprot | |
| K682 | Acetylation | Uniprot | |
| S694 | Phosphorylation | Q14012 (CAMK1) | Uniprot |
| S700 | Phosphorylation | Uniprot | |
| K702 | Ubiquitination | Uniprot | |
| S703 | Phosphorylation | Uniprot | |
| T704 | Phosphorylation | Uniprot | |
| K715 | Ubiquitination | Uniprot | |
| Y720 | Phosphorylation | Uniprot | |
| T722 | Phosphorylation | Uniprot | |
| S723 | Phosphorylation | Uniprot | |
| K725 | Ubiquitination | Uniprot | |
| S761 | Phosphorylation | P05129 (PRKCG) | Uniprot |
| S763 | Phosphorylation | Uniprot | |
| S784 | Phosphorylation | Uniprot | |
| Y785 | Phosphorylation | Uniprot | |
| S800 | Phosphorylation | Uniprot |
研究背景
Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions (By similarity). May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons.
Phosphorylated by PTK2/FAK1; this promotes interaction with RAC1 (By similarity). Phosphorylated on Ser-694 by CaMK1; enhancement of GEF activity and downstream activation of RAC1.
Cell junction>Focal adhesion. Cell projection>Ruffle. Cytoplasm>Cell cortex. Cell projection>Lamellipodium.
Note: Detected at cell adhesions. A small proportion is detected at focal adhesions.
Interacts with PAK kinases through the SH3 domain. Interacts with GIT1 and TGFB1I1. Interacts with PTK2/FAK1 and RAC1. Interacts with ITCH and PARVB (By similarity). Interacts with unphosphorylated PAK1. Interacts with SCRIB; interaction is direct and may play a role in regulation of apoptosis. Interacts with FRMPD4 (via N-terminus). Interacts with CaMK1. Interacts with BIN2. Isoform 1 and isoform 5 interact with SNX27. Interacts with YWHAZ (By similarity).
研究領域
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
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