RNUXA Antibody - #DF4446
| 製品: | RNUXA Antibody |
| カタログ: | DF4446 |
| タンパク質の説明: | Rabbit polyclonal antibody to RNUXA |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 48 KD; 44kD(Calculated). |
| ユニプロット: | Q9H814 |
| RRID: | AB_2836801 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4446, RRID:AB_2836801.
折りたたみ／展開
FLJ13193; PHAX; PHAX_HUMAN; Phosphorylated adapter RNA export protein; Phosphorylated adaptor for RNA export; RNA U small nuclear RNA export adapter protein; RNA U, small nuclear RNA export adapter (phosphorylation regulated); RNUXA;
免疫原
- Q9H814 PHAX_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALEVGDMEDGQLSDSDSDMTVAPSDRPLQLPKVLGGDSAMRAFQNTATACAPVSHYRAVESVDSSEESFSDSDDDSCLWKRKRQKCFNPPPKPEPFQFGQSSQKPPVAGGKKINNIWGAVLQEQNQDAVATELGILGMEGTIDRSRQSETYNYLLAKKLRKESQEHTKDLDKELDEYMHGGKKMGSKEEENGQGHLKRKRPVKDRLGNRPEMNYKGRYEITAEDSQEKVADEISFRLQEPKKDLIARVVRIIGNKKAIELLMETAEVEQNGGLFIMNGSRRRTPGGVFLNLLKNTPSISEEQIKDIFYIENQKEYENKKAARKRRTQVLGKKMKQAIKSLNFQEDDDTSRETFASDTNEALASLDESQEGHAEAKLEAEEAIEVDHSHDLDIF
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H814 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| T21 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| Y57 | Phosphorylation | Uniprot | |
| S65 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| S69 | Phosphorylation | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| S73 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| K105 | Acetylation | Uniprot | |
| K112 | Acetylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| T151 | Phosphorylation | Uniprot | |
| Y152 | Phosphorylation | Uniprot | |
| Y154 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| K169 | Methylation | Uniprot | |
| Y178 | Phosphorylation | Uniprot | |
| K183 | Acetylation | Uniprot | |
| K184 | Acetylation | Uniprot | |
| S226 | Phosphorylation | Uniprot | |
| T296 | Phosphorylation | Uniprot | |
| Y316 | Phosphorylation | Uniprot | |
| T327 | Phosphorylation | Uniprot | |
| K339 | Ubiquitination | Uniprot | |
| T349 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| T353 | Phosphorylation | Uniprot | |
| S356 | Phosphorylation | Uniprot | |
| T358 | Phosphorylation | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| S388 | Phosphorylation | Uniprot |
研究背景
A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner (By similarity). Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA.
Phosphorylated in the nucleus. Dephosphorylated in the cytoplasm (By similarity).
Nucleus>Nucleoplasm. Nucleus>Cajal body. Cytoplasm.
Note: Located in the nucleoplasm and Cajal bodies. Shuttles between the nucleus and the cytoplasm. Shuttles between the nucleoplasm and Cajal bodies.
Found in a U snRNA export complex with PHAX/RNUXA, NCBP1/CBP80, NCBP2/CBP20, RAN, XPO1 and m7G-capped RNA. Part of a precomplex with PHAX/RNUXA, NCBP1/CBP80, NCBP2/CBP20 and m7G-capped RNA. Interacts with NCBP1/CBP80 (By similarity). Found in a complex with snoRNA. Interacts with NCBP2/CBP20.
Belongs to the PHAX family.
研究領域
· Genetic Information Processing > Translation > RNA transport.
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