製品: RUFY1 Antibody
カタログ: DF4448
タンパク質の説明: Rabbit polyclonal antibody to RUFY1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse
予測: Pig, Rabbit, Dog, Chicken
分子量: 80 KD; 80kD(Calculated).
ユニプロット: Q96T51
RRID: AB_2836803

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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Rabbit(100%), Dog(89%), Chicken(80%)
クローナリティ:
Polyclonal
特異性:
RUFY1 Antibody detects endogenous levels of total RUFY1.
RRID:
AB_2836803
引用形式: Affinity Biosciences Cat# DF4448, RRID:AB_2836803.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

3000002E04Rik; FLJ22251; FYVE finger protein EIP1; FYVE-finger protein EIP1; La binding protein 1; La-binding protein 1; OTTHUMP00000161521; Rab4 interacting protein; Rab4-interacting protein; RABIP4; RUFY1; RUFY1_HUMAN; RUN and FYVE domain containing 1; RUN and FYVE domain-containing protein 1; RUN and FYVE domains containing protein 1; ZFYVE12; Zinc finger FYVE domain containing protein 12; Zinc finger FYVE domain-containing protein 12;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q96T51 RUFY1_HUMAN:

Broadly expressed, with highest levels in lung, testis, kidney and brain.

タンパク質配列:
MADREGGCAAGRGRELEPELEPGPGPGSALEPGEEFEIVDRSQLPGPGDLRSATRPRAAEGWSAPILTLARRATGNLSASCGSALRAAAGLGGGDSGDGTARAASKCQMMEERANLMHMMKLSIKVLLQSALSLGRSLDADHAPLQQFFVVMEHCLKHGLKVKKSFIGQNKSFFGPLELVEKLCPEASDIATSVRNLPELKTAVGRGRAWLYLALMQKKLADYLKVLIDNKHLLSEFYEPEALMMEEEGMVIVGLLVGLNVLDANLCLKGEDLDSQVGVIDFSLYLKDVQDLDGGKEHERITDVLDQKNYVEELNRHLSCTVGDLQTKIDGLEKTNSKLQEELSAATDRICSLQEEQQQLREQNELIRERSEKSVEITKQDTKVELETYKQTRQGLDEMYSDVWKQLKEEKKVRLELEKELELQIGMKTEMEIAMKLLEKDTHEKQDTLVALRQQLEEVKAINLQMFHKAQNAESSLQQKNEAITSFEGKTNQVMSSMKQMEERLQHSERARQGAEERSHKLQQELGGRIGALQLQLSQLHEQCSSLEKELKSEKEQRQALQRELQHEKDTSSLLRMELQQVEGLKKELRELQDEKAELQKICEEQEQALQEMGLHLSQSKLKMEDIKEVNQALKGHAWLKDDEATHCRQCEKEFSISRRKHHCRNCGHIFCNTCSSNELALPSYPKPVRVCDSCHTLLLQRCSSTAS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
100
Dog
89
Chicken
80
Horse
78
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96T51 基板として

Site PTM Type Enzyme
R12 Methylation
R14 Methylation
S63 Phosphorylation
T74 Phosphorylation
S78 Phosphorylation
S80 Phosphorylation
S83 Phosphorylation
S123 Phosphorylation
K164 Ubiquitination
S165 Phosphorylation
K171 Ubiquitination
S172 Phosphorylation
K182 Ubiquitination
C184 S-Nitrosylation
S188 Phosphorylation
T192 Phosphorylation
S193 Phosphorylation
K225 Methylation
K308 Ubiquitination
S319 Phosphorylation
C320 S-Nitrosylation
K338 Ubiquitination
S344 Phosphorylation
T347 Phosphorylation
R349 Methylation
S352 Phosphorylation
K379 Acetylation
Y389 Phosphorylation P51813 (BMX)
K390 Ubiquitination
Y400 Phosphorylation P51813 (BMX)
S401 Phosphorylation
K445 Ubiquitination
K460 Ubiquitination
K469 Ubiquitination
K480 Ubiquitination
S486 Phosphorylation
K490 Ubiquitination
K499 Ubiquitination
K521 Ubiquitination
K555 Methylation
K569 Ubiquitination
S618 Phosphorylation
S620 Phosphorylation
K621 Ubiquitination
K635 Ubiquitination
K641 Ubiquitination
S694 Phosphorylation

研究背景

機能:

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.

PTMs:

Phosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting.

細胞の位置付け:

Cytoplasm. Early endosome membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Broadly expressed, with highest levels in lung, testis, kidney and brain.

サブユニット構造:

Interacts with BMX. May interact with SSB. Interacts with RAB4 and RAB5 that have been activated by GTP-binding.

タンパク質ファミリー:

The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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